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Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus
Cortical microtubules (MTs) are evolutionarily conserved cytoskeletal components with specialized roles in plants, including regulation of cell wall biogenesis. MT functions and dynamics are dictated by the composition of their monomeric subunits, α- (TUA) and β-tubulins (TUB), which in animals and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061773/ https://www.ncbi.nlm.nih.gov/pubmed/27790223 http://dx.doi.org/10.3389/fpls.2016.01493 |
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author | Hu, Hao Gu, Xi Xue, Liang-Jiao Swamy, Prashant S. Harding, Scott A. Tsai, Chung-Jui |
author_facet | Hu, Hao Gu, Xi Xue, Liang-Jiao Swamy, Prashant S. Harding, Scott A. Tsai, Chung-Jui |
author_sort | Hu, Hao |
collection | PubMed |
description | Cortical microtubules (MTs) are evolutionarily conserved cytoskeletal components with specialized roles in plants, including regulation of cell wall biogenesis. MT functions and dynamics are dictated by the composition of their monomeric subunits, α- (TUA) and β-tubulins (TUB), which in animals and protists are subject to both transcriptional regulation and post-translational modifications (PTM). While spatiotemporal regulation of tubulin gene expression has been reported in plants, whether and to what extent tubulin PTMs occur in these species remain poorly understood. We chose the woody perennial Populus for investigation of tubulin PTMs in this study, with a particular focus on developing xylem where high tubulin transcript levels support MT-dependent secondary cell wall deposition. Mass spectrometry and immunodetection concurred that detyrosination, non-tyrosination and glutamylation were essentially absent in tubulins isolated from wood-forming tissues of P. deltoides and P. tremula ×alba. Label-free quantification of tubulin isotypes and RNA-Seq estimation of tubulin transcript abundance were largely consistent with transcriptional regulation. However, two TUB isotypes were detected at noticeably lower levels than expected based on RNA-Seq transcript abundance in both Populus species. These findings led us to conclude that MT composition during wood formation depends exclusively on transcriptional and, to a lesser extent, translational regulation of tubulin isotypes. |
format | Online Article Text |
id | pubmed-5061773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50617732016-10-27 Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus Hu, Hao Gu, Xi Xue, Liang-Jiao Swamy, Prashant S. Harding, Scott A. Tsai, Chung-Jui Front Plant Sci Plant Science Cortical microtubules (MTs) are evolutionarily conserved cytoskeletal components with specialized roles in plants, including regulation of cell wall biogenesis. MT functions and dynamics are dictated by the composition of their monomeric subunits, α- (TUA) and β-tubulins (TUB), which in animals and protists are subject to both transcriptional regulation and post-translational modifications (PTM). While spatiotemporal regulation of tubulin gene expression has been reported in plants, whether and to what extent tubulin PTMs occur in these species remain poorly understood. We chose the woody perennial Populus for investigation of tubulin PTMs in this study, with a particular focus on developing xylem where high tubulin transcript levels support MT-dependent secondary cell wall deposition. Mass spectrometry and immunodetection concurred that detyrosination, non-tyrosination and glutamylation were essentially absent in tubulins isolated from wood-forming tissues of P. deltoides and P. tremula ×alba. Label-free quantification of tubulin isotypes and RNA-Seq estimation of tubulin transcript abundance were largely consistent with transcriptional regulation. However, two TUB isotypes were detected at noticeably lower levels than expected based on RNA-Seq transcript abundance in both Populus species. These findings led us to conclude that MT composition during wood formation depends exclusively on transcriptional and, to a lesser extent, translational regulation of tubulin isotypes. Frontiers Media S.A. 2016-10-13 /pmc/articles/PMC5061773/ /pubmed/27790223 http://dx.doi.org/10.3389/fpls.2016.01493 Text en Copyright © 2016 Hu, Gu, Xue, Swamy, Harding and Tsai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Hu, Hao Gu, Xi Xue, Liang-Jiao Swamy, Prashant S. Harding, Scott A. Tsai, Chung-Jui Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus |
title | Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus |
title_full | Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus |
title_fullStr | Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus |
title_full_unstemmed | Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus |
title_short | Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus |
title_sort | tubulin c-terminal post-translational modifications do not occur in wood forming tissue of populus |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061773/ https://www.ncbi.nlm.nih.gov/pubmed/27790223 http://dx.doi.org/10.3389/fpls.2016.01493 |
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