Isozymes of AMP-Deaminase in Muscles Myasthenia Gravis Patients

Similar symptoms observed in Myasthenia gravis (MG) can be also detected in the case of skeletal muscle AMP-deaminase deficiency. We compared the activity and expression of AMP-deaminase (AMPD) products in skeletal muscles of MG patients and MG-free individuals. The activity of AMP-deaminase in the...

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Autores principales: Rybakowska, Iwona M., Bakuła, Stanisław, Kaletha, Krystian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061832/
https://www.ncbi.nlm.nih.gov/pubmed/27795699
http://dx.doi.org/10.1007/s10989-016-9533-9
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author Rybakowska, Iwona M.
Bakuła, Stanisław
Kaletha, Krystian
author_facet Rybakowska, Iwona M.
Bakuła, Stanisław
Kaletha, Krystian
author_sort Rybakowska, Iwona M.
collection PubMed
description Similar symptoms observed in Myasthenia gravis (MG) can be also detected in the case of skeletal muscle AMP-deaminase deficiency. We compared the activity and expression of AMP-deaminase (AMPD) products in skeletal muscles of MG patients and MG-free individuals. The activity of AMP-deaminase in the muscles of MG patients was significantly higher than in the controls and was 2.05 µmol/min/mg protein (±0.31). The two groups differ in level of AMPD product expression. Furthermore in MG-group molecular size of isoform AMPD1 is 90 kDa in contrast to MG-free group where is present 70 kDa isoform of enzyme. The data suggests that the disturbances in transmission of neuronal signaling, taking place in the skeletal muscles of MG patients, may also change energetic metabolism of the affected muscles by changing molecular mass of isoform.
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spelling pubmed-50618322016-10-26 Isozymes of AMP-Deaminase in Muscles Myasthenia Gravis Patients Rybakowska, Iwona M. Bakuła, Stanisław Kaletha, Krystian Int J Pept Res Ther Article Similar symptoms observed in Myasthenia gravis (MG) can be also detected in the case of skeletal muscle AMP-deaminase deficiency. We compared the activity and expression of AMP-deaminase (AMPD) products in skeletal muscles of MG patients and MG-free individuals. The activity of AMP-deaminase in the muscles of MG patients was significantly higher than in the controls and was 2.05 µmol/min/mg protein (±0.31). The two groups differ in level of AMPD product expression. Furthermore in MG-group molecular size of isoform AMPD1 is 90 kDa in contrast to MG-free group where is present 70 kDa isoform of enzyme. The data suggests that the disturbances in transmission of neuronal signaling, taking place in the skeletal muscles of MG patients, may also change energetic metabolism of the affected muscles by changing molecular mass of isoform. Springer Netherlands 2016-05-13 2016 /pmc/articles/PMC5061832/ /pubmed/27795699 http://dx.doi.org/10.1007/s10989-016-9533-9 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Rybakowska, Iwona M.
Bakuła, Stanisław
Kaletha, Krystian
Isozymes of AMP-Deaminase in Muscles Myasthenia Gravis Patients
title Isozymes of AMP-Deaminase in Muscles Myasthenia Gravis Patients
title_full Isozymes of AMP-Deaminase in Muscles Myasthenia Gravis Patients
title_fullStr Isozymes of AMP-Deaminase in Muscles Myasthenia Gravis Patients
title_full_unstemmed Isozymes of AMP-Deaminase in Muscles Myasthenia Gravis Patients
title_short Isozymes of AMP-Deaminase in Muscles Myasthenia Gravis Patients
title_sort isozymes of amp-deaminase in muscles myasthenia gravis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061832/
https://www.ncbi.nlm.nih.gov/pubmed/27795699
http://dx.doi.org/10.1007/s10989-016-9533-9
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