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Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi

Termites are considered one of the most efficient decomposers of lignocelluloses on Earth due to their ability to produce, along with its microbial symbionts, a repertoire of carbohydrate-active enzymes (CAZymes). Recently, a set of Pro-oxidant, Antioxidant, and Detoxification enzymes (PAD) were als...

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Autores principales: Franco Cairo, João P. L., Carazzolle, Marcelo F., Leonardo, Flávia C., Mofatto, Luciana S., Brenelli, Lívia B., Gonçalves, Thiago A., Uchima, Cristiane A., Domingues, Romênia R., Alvarez, Thabata M., Tramontina, Robson, Vidal, Ramon O., Costa, Fernando F., Costa-Leonardo, Ana M., Paes Leme, Adriana F., Pereira, Gonçalo A. G., Squina, Fabio M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061848/
https://www.ncbi.nlm.nih.gov/pubmed/27790186
http://dx.doi.org/10.3389/fmicb.2016.01518
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author Franco Cairo, João P. L.
Carazzolle, Marcelo F.
Leonardo, Flávia C.
Mofatto, Luciana S.
Brenelli, Lívia B.
Gonçalves, Thiago A.
Uchima, Cristiane A.
Domingues, Romênia R.
Alvarez, Thabata M.
Tramontina, Robson
Vidal, Ramon O.
Costa, Fernando F.
Costa-Leonardo, Ana M.
Paes Leme, Adriana F.
Pereira, Gonçalo A. G.
Squina, Fabio M.
author_facet Franco Cairo, João P. L.
Carazzolle, Marcelo F.
Leonardo, Flávia C.
Mofatto, Luciana S.
Brenelli, Lívia B.
Gonçalves, Thiago A.
Uchima, Cristiane A.
Domingues, Romênia R.
Alvarez, Thabata M.
Tramontina, Robson
Vidal, Ramon O.
Costa, Fernando F.
Costa-Leonardo, Ana M.
Paes Leme, Adriana F.
Pereira, Gonçalo A. G.
Squina, Fabio M.
author_sort Franco Cairo, João P. L.
collection PubMed
description Termites are considered one of the most efficient decomposers of lignocelluloses on Earth due to their ability to produce, along with its microbial symbionts, a repertoire of carbohydrate-active enzymes (CAZymes). Recently, a set of Pro-oxidant, Antioxidant, and Detoxification enzymes (PAD) were also correlated with the metabolism of carbohydrates and lignin in termites. The lower termite Coptotermes gestroi is considered the main urban pest in Brazil, causing damage to wood constructions. Recently, analysis of the enzymatic repertoire of C. gestroi unveiled the presence of different CAZymes. Because the gene profile of CAZy/PAD enzymes endogenously synthesized by C. gestroi and also by their symbiotic protists remains unclear, the aim of this study was to explore the eukaryotic repertoire of these enzymes in worker and soldier castes of C. gestroi. Our findings showed that worker and soldier castes present similar repertoires of CAZy/PAD enzymes, and also confirmed that endo-glucanases (GH9) and beta-glucosidases (GH1) were the most important glycoside hydrolase families related to lignocellulose degradation in both castes. Classical cellulases such as exo-glucanases (GH7) and endo-glucanases (GH5 and GH45), as well as classical xylanases (GH10 and GH11), were found in both castes only taxonomically related to protists, highlighting the importance of symbiosis in C. gestroi. Moreover, our analysis revealed the presence of Auxiliary Activity enzyme families (AAs), which could be related to lignin modifications in termite digestomes. In conclusion, this report expanded the knowledge on genes and proteins related to CAZy/PAD enzymes from worker and soldier castes of lower termites, revealing new potential enzyme candidates for second-generation biofuel processes.
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spelling pubmed-50618482016-10-27 Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi Franco Cairo, João P. L. Carazzolle, Marcelo F. Leonardo, Flávia C. Mofatto, Luciana S. Brenelli, Lívia B. Gonçalves, Thiago A. Uchima, Cristiane A. Domingues, Romênia R. Alvarez, Thabata M. Tramontina, Robson Vidal, Ramon O. Costa, Fernando F. Costa-Leonardo, Ana M. Paes Leme, Adriana F. Pereira, Gonçalo A. G. Squina, Fabio M. Front Microbiol Microbiology Termites are considered one of the most efficient decomposers of lignocelluloses on Earth due to their ability to produce, along with its microbial symbionts, a repertoire of carbohydrate-active enzymes (CAZymes). Recently, a set of Pro-oxidant, Antioxidant, and Detoxification enzymes (PAD) were also correlated with the metabolism of carbohydrates and lignin in termites. The lower termite Coptotermes gestroi is considered the main urban pest in Brazil, causing damage to wood constructions. Recently, analysis of the enzymatic repertoire of C. gestroi unveiled the presence of different CAZymes. Because the gene profile of CAZy/PAD enzymes endogenously synthesized by C. gestroi and also by their symbiotic protists remains unclear, the aim of this study was to explore the eukaryotic repertoire of these enzymes in worker and soldier castes of C. gestroi. Our findings showed that worker and soldier castes present similar repertoires of CAZy/PAD enzymes, and also confirmed that endo-glucanases (GH9) and beta-glucosidases (GH1) were the most important glycoside hydrolase families related to lignocellulose degradation in both castes. Classical cellulases such as exo-glucanases (GH7) and endo-glucanases (GH5 and GH45), as well as classical xylanases (GH10 and GH11), were found in both castes only taxonomically related to protists, highlighting the importance of symbiosis in C. gestroi. Moreover, our analysis revealed the presence of Auxiliary Activity enzyme families (AAs), which could be related to lignin modifications in termite digestomes. In conclusion, this report expanded the knowledge on genes and proteins related to CAZy/PAD enzymes from worker and soldier castes of lower termites, revealing new potential enzyme candidates for second-generation biofuel processes. Frontiers Media S.A. 2016-10-13 /pmc/articles/PMC5061848/ /pubmed/27790186 http://dx.doi.org/10.3389/fmicb.2016.01518 Text en Copyright © 2016 Franco Cairo, Carazzolle, Leonardo, Mofatto, Brenelli, Gonçalves, Uchima, Domingues, Alvarez, Tramontina, Vidal, Costa, Costa-Leonardo, Paes Leme, Pereira and Squina. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Franco Cairo, João P. L.
Carazzolle, Marcelo F.
Leonardo, Flávia C.
Mofatto, Luciana S.
Brenelli, Lívia B.
Gonçalves, Thiago A.
Uchima, Cristiane A.
Domingues, Romênia R.
Alvarez, Thabata M.
Tramontina, Robson
Vidal, Ramon O.
Costa, Fernando F.
Costa-Leonardo, Ana M.
Paes Leme, Adriana F.
Pereira, Gonçalo A. G.
Squina, Fabio M.
Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi
title Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi
title_full Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi
title_fullStr Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi
title_full_unstemmed Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi
title_short Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi
title_sort expanding the knowledge on lignocellulolytic and redox enzymes of worker and soldier castes from the lower termite coptotermes gestroi
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061848/
https://www.ncbi.nlm.nih.gov/pubmed/27790186
http://dx.doi.org/10.3389/fmicb.2016.01518
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