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Life without Division: Physiology of Escherichia coli FtsZ-Deprived Filaments

When deprived of FtsZ, Escherichia coli cells (VIP205) grown in liquid form long nonseptated filaments due to their inability to assemble an FtsZ ring and their failure to recruit subsequent divisome components. These filaments fail to produce colonies on solid medium, in which synthesis of FtsZ is...

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Autores principales: Sánchez-Gorostiaga, Alicia, Palacios, Pilar, Martínez-Arteaga, Rocío, Sánchez, Manuel, Casanova, Mercedes, Vicente, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061873/
https://www.ncbi.nlm.nih.gov/pubmed/27729511
http://dx.doi.org/10.1128/mBio.01620-16
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author Sánchez-Gorostiaga, Alicia
Palacios, Pilar
Martínez-Arteaga, Rocío
Sánchez, Manuel
Casanova, Mercedes
Vicente, Miguel
author_facet Sánchez-Gorostiaga, Alicia
Palacios, Pilar
Martínez-Arteaga, Rocío
Sánchez, Manuel
Casanova, Mercedes
Vicente, Miguel
author_sort Sánchez-Gorostiaga, Alicia
collection PubMed
description When deprived of FtsZ, Escherichia coli cells (VIP205) grown in liquid form long nonseptated filaments due to their inability to assemble an FtsZ ring and their failure to recruit subsequent divisome components. These filaments fail to produce colonies on solid medium, in which synthesis of FtsZ is induced, upon being diluted by a factor greater than 4. However, once the initial FtsZ levels are recovered in liquid culture, they resume division, and their plating efficiency returns to normal. The potential septation sites generated in the FtsZ-deprived filaments are not annihilated, and once sufficient FtsZ is accumulated, they all become active and divide to produce cells of normal length. FtsZ-deprived cells accumulate defects in their physiology, including an abnormally high number of unsegregated nucleoids that may result from the misplacement of FtsK. Their membrane integrity becomes compromised and the amount of membrane proteins, such as FtsK and ZipA, increases. FtsZ-deprived cells also show an altered expression pattern, namely, transcription of several genes responding to DNA damage increases, whereas transcription of some ribosomal or global transcriptional regulators decreases. We propose that the changes caused by the depletion of FtsZ, besides stopping division, weaken the cell, diminishing its resiliency to minor challenges, such as dilution stress.
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spelling pubmed-50618732016-10-13 Life without Division: Physiology of Escherichia coli FtsZ-Deprived Filaments Sánchez-Gorostiaga, Alicia Palacios, Pilar Martínez-Arteaga, Rocío Sánchez, Manuel Casanova, Mercedes Vicente, Miguel mBio Research Article When deprived of FtsZ, Escherichia coli cells (VIP205) grown in liquid form long nonseptated filaments due to their inability to assemble an FtsZ ring and their failure to recruit subsequent divisome components. These filaments fail to produce colonies on solid medium, in which synthesis of FtsZ is induced, upon being diluted by a factor greater than 4. However, once the initial FtsZ levels are recovered in liquid culture, they resume division, and their plating efficiency returns to normal. The potential septation sites generated in the FtsZ-deprived filaments are not annihilated, and once sufficient FtsZ is accumulated, they all become active and divide to produce cells of normal length. FtsZ-deprived cells accumulate defects in their physiology, including an abnormally high number of unsegregated nucleoids that may result from the misplacement of FtsK. Their membrane integrity becomes compromised and the amount of membrane proteins, such as FtsK and ZipA, increases. FtsZ-deprived cells also show an altered expression pattern, namely, transcription of several genes responding to DNA damage increases, whereas transcription of some ribosomal or global transcriptional regulators decreases. We propose that the changes caused by the depletion of FtsZ, besides stopping division, weaken the cell, diminishing its resiliency to minor challenges, such as dilution stress. American Society for Microbiology 2016-10-11 /pmc/articles/PMC5061873/ /pubmed/27729511 http://dx.doi.org/10.1128/mBio.01620-16 Text en Copyright © 2016 Sánchez-Gorostiaga et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Sánchez-Gorostiaga, Alicia
Palacios, Pilar
Martínez-Arteaga, Rocío
Sánchez, Manuel
Casanova, Mercedes
Vicente, Miguel
Life without Division: Physiology of Escherichia coli FtsZ-Deprived Filaments
title Life without Division: Physiology of Escherichia coli FtsZ-Deprived Filaments
title_full Life without Division: Physiology of Escherichia coli FtsZ-Deprived Filaments
title_fullStr Life without Division: Physiology of Escherichia coli FtsZ-Deprived Filaments
title_full_unstemmed Life without Division: Physiology of Escherichia coli FtsZ-Deprived Filaments
title_short Life without Division: Physiology of Escherichia coli FtsZ-Deprived Filaments
title_sort life without division: physiology of escherichia coli ftsz-deprived filaments
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061873/
https://www.ncbi.nlm.nih.gov/pubmed/27729511
http://dx.doi.org/10.1128/mBio.01620-16
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