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A Transcriptomic Signature of Mouse Liver Progenitor Cells

Liver progenitor cells (LPCs) can proliferate extensively, are able to differentiate into hepatocytes and cholangiocytes, and contribute to liver regeneration. The presence of LPCs, however, often accompanies liver disease and hepatocellular carcinoma (HCC), indicating that they may be a cancer stem...

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Autores principales: Passman, Adam M., Low, Jasmine, London, Roslyn, Tirnitz-Parker, Janina E. E., Miyajima, Atsushi, Tanaka, Minoru, Strick-Marchand, Helene, Darlington, Gretchen J., Finch-Edmondson, Megan, Ochsner, Scott, Zhu, Cornelia, Whelan, James, Callus, Bernard A., Yeoh, George C. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061959/
https://www.ncbi.nlm.nih.gov/pubmed/27777588
http://dx.doi.org/10.1155/2016/5702873
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author Passman, Adam M.
Low, Jasmine
London, Roslyn
Tirnitz-Parker, Janina E. E.
Miyajima, Atsushi
Tanaka, Minoru
Strick-Marchand, Helene
Darlington, Gretchen J.
Finch-Edmondson, Megan
Ochsner, Scott
Zhu, Cornelia
Whelan, James
Callus, Bernard A.
Yeoh, George C. T.
author_facet Passman, Adam M.
Low, Jasmine
London, Roslyn
Tirnitz-Parker, Janina E. E.
Miyajima, Atsushi
Tanaka, Minoru
Strick-Marchand, Helene
Darlington, Gretchen J.
Finch-Edmondson, Megan
Ochsner, Scott
Zhu, Cornelia
Whelan, James
Callus, Bernard A.
Yeoh, George C. T.
author_sort Passman, Adam M.
collection PubMed
description Liver progenitor cells (LPCs) can proliferate extensively, are able to differentiate into hepatocytes and cholangiocytes, and contribute to liver regeneration. The presence of LPCs, however, often accompanies liver disease and hepatocellular carcinoma (HCC), indicating that they may be a cancer stem cell. Understanding LPC biology and establishing a sensitive, rapid, and reliable method to detect their presence in the liver will assist diagnosis and facilitate monitoring of treatment outcomes in patients with liver pathologies. A transcriptomic meta-analysis of over 400 microarrays was undertaken to compare LPC lines against datasets of muscle and embryonic stem cell lines, embryonic and developed liver (DL), and HCC. Three gene clusters distinguishing LPCs from other liver cell types were identified. Pathways overrepresented in these clusters denote the proliferative nature of LPCs and their association with HCC. Our analysis also revealed 26 novel markers, LPC markers, including Mcm2 and Ltbp3, and eight known LPC markers, including M2pk and Ncam. These markers specified the presence of LPCs in pathological liver tissue by qPCR and correlated with LPC abundance determined using immunohistochemistry. These results showcase the value of global transcript profiling to identify pathways and markers that may be used to detect LPCs in injured or diseased liver.
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spelling pubmed-50619592016-10-24 A Transcriptomic Signature of Mouse Liver Progenitor Cells Passman, Adam M. Low, Jasmine London, Roslyn Tirnitz-Parker, Janina E. E. Miyajima, Atsushi Tanaka, Minoru Strick-Marchand, Helene Darlington, Gretchen J. Finch-Edmondson, Megan Ochsner, Scott Zhu, Cornelia Whelan, James Callus, Bernard A. Yeoh, George C. T. Stem Cells Int Research Article Liver progenitor cells (LPCs) can proliferate extensively, are able to differentiate into hepatocytes and cholangiocytes, and contribute to liver regeneration. The presence of LPCs, however, often accompanies liver disease and hepatocellular carcinoma (HCC), indicating that they may be a cancer stem cell. Understanding LPC biology and establishing a sensitive, rapid, and reliable method to detect their presence in the liver will assist diagnosis and facilitate monitoring of treatment outcomes in patients with liver pathologies. A transcriptomic meta-analysis of over 400 microarrays was undertaken to compare LPC lines against datasets of muscle and embryonic stem cell lines, embryonic and developed liver (DL), and HCC. Three gene clusters distinguishing LPCs from other liver cell types were identified. Pathways overrepresented in these clusters denote the proliferative nature of LPCs and their association with HCC. Our analysis also revealed 26 novel markers, LPC markers, including Mcm2 and Ltbp3, and eight known LPC markers, including M2pk and Ncam. These markers specified the presence of LPCs in pathological liver tissue by qPCR and correlated with LPC abundance determined using immunohistochemistry. These results showcase the value of global transcript profiling to identify pathways and markers that may be used to detect LPCs in injured or diseased liver. Hindawi Publishing Corporation 2016 2016-10-03 /pmc/articles/PMC5061959/ /pubmed/27777588 http://dx.doi.org/10.1155/2016/5702873 Text en Copyright © 2016 Adam M. Passman et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Passman, Adam M.
Low, Jasmine
London, Roslyn
Tirnitz-Parker, Janina E. E.
Miyajima, Atsushi
Tanaka, Minoru
Strick-Marchand, Helene
Darlington, Gretchen J.
Finch-Edmondson, Megan
Ochsner, Scott
Zhu, Cornelia
Whelan, James
Callus, Bernard A.
Yeoh, George C. T.
A Transcriptomic Signature of Mouse Liver Progenitor Cells
title A Transcriptomic Signature of Mouse Liver Progenitor Cells
title_full A Transcriptomic Signature of Mouse Liver Progenitor Cells
title_fullStr A Transcriptomic Signature of Mouse Liver Progenitor Cells
title_full_unstemmed A Transcriptomic Signature of Mouse Liver Progenitor Cells
title_short A Transcriptomic Signature of Mouse Liver Progenitor Cells
title_sort transcriptomic signature of mouse liver progenitor cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061959/
https://www.ncbi.nlm.nih.gov/pubmed/27777588
http://dx.doi.org/10.1155/2016/5702873
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