NO-Tryptophan: A New Small Molecule Located in the Rat Brain

A highly specific monoclonal antibody directed against nitric oxide-tryptophan (NO-W) with good affinity (10(-9) M) and specificity was developed. In the rat brain, using an indirect immunoperoxidase technique, cell bodies containing NO-W were exclusively found in the intermediate and dorsal parts o...

Descripción completa

Detalles Bibliográficos
Autores principales: Mangas, A., Yajeya, J., González, N., Duleu, S., Geffard, M., Coveñas, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2016
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062636/
https://www.ncbi.nlm.nih.gov/pubmed/27734994
http://dx.doi.org/10.4081/ejh.2016.2692
_version_ 1782459818622058496
author Mangas, A.
Yajeya, J.
González, N.
Duleu, S.
Geffard, M.
Coveñas, R.
author_facet Mangas, A.
Yajeya, J.
González, N.
Duleu, S.
Geffard, M.
Coveñas, R.
author_sort Mangas, A.
collection PubMed
description A highly specific monoclonal antibody directed against nitric oxide-tryptophan (NO-W) with good affinity (10(-9) M) and specificity was developed. In the rat brain, using an indirect immunoperoxidase technique, cell bodies containing NO-W were exclusively found in the intermediate and dorsal parts of the lateral septal nucleus. No immunoreactive fibres were found in the rat brain. This work reports the first visualization and the morphological characteristics of cell bodies containing NO-W in the mammalian brain. The restricted distribution of NO-W in the rat brain suggests that this molecule could be involved in specific physiological mechanisms.
format Online
Article
Text
id pubmed-5062636
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher PAGEPress Publications, Pavia, Italy
record_format MEDLINE/PubMed
spelling pubmed-50626362016-10-14 NO-Tryptophan: A New Small Molecule Located in the Rat Brain Mangas, A. Yajeya, J. González, N. Duleu, S. Geffard, M. Coveñas, R. Eur J Histochem Original Paper A highly specific monoclonal antibody directed against nitric oxide-tryptophan (NO-W) with good affinity (10(-9) M) and specificity was developed. In the rat brain, using an indirect immunoperoxidase technique, cell bodies containing NO-W were exclusively found in the intermediate and dorsal parts of the lateral septal nucleus. No immunoreactive fibres were found in the rat brain. This work reports the first visualization and the morphological characteristics of cell bodies containing NO-W in the mammalian brain. The restricted distribution of NO-W in the rat brain suggests that this molecule could be involved in specific physiological mechanisms. PAGEPress Publications, Pavia, Italy 2016-09-22 /pmc/articles/PMC5062636/ /pubmed/27734994 http://dx.doi.org/10.4081/ejh.2016.2692 Text en ©Copyright A. Mangas et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Mangas, A.
Yajeya, J.
González, N.
Duleu, S.
Geffard, M.
Coveñas, R.
NO-Tryptophan: A New Small Molecule Located in the Rat Brain
title NO-Tryptophan: A New Small Molecule Located in the Rat Brain
title_full NO-Tryptophan: A New Small Molecule Located in the Rat Brain
title_fullStr NO-Tryptophan: A New Small Molecule Located in the Rat Brain
title_full_unstemmed NO-Tryptophan: A New Small Molecule Located in the Rat Brain
title_short NO-Tryptophan: A New Small Molecule Located in the Rat Brain
title_sort no-tryptophan: a new small molecule located in the rat brain
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062636/
https://www.ncbi.nlm.nih.gov/pubmed/27734994
http://dx.doi.org/10.4081/ejh.2016.2692
work_keys_str_mv AT mangasa notryptophananewsmallmoleculelocatedintheratbrain
AT yajeyaj notryptophananewsmallmoleculelocatedintheratbrain
AT gonzalezn notryptophananewsmallmoleculelocatedintheratbrain
AT duleus notryptophananewsmallmoleculelocatedintheratbrain
AT geffardm notryptophananewsmallmoleculelocatedintheratbrain
AT covenasr notryptophananewsmallmoleculelocatedintheratbrain

Ejemplares similares