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Wnt/β-catenin signaling pathway activation is required for proliferation of chicken primordial germ cells in vitro
Here, we investigated the role of the Wnt/β-catenin signaling pathway in chicken primordial germ cells (PGCs) in vitro. We confirmed the expression of Wnt signaling pathway-related genes and the localization of β-catenin in the nucleus, revealing that this pathway is potentially activated in chicken...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062643/ https://www.ncbi.nlm.nih.gov/pubmed/27687983 http://dx.doi.org/10.1038/srep34510 |
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author | Lee, Hyung Chul Lim, Sumi Han, Jae Yong |
author_facet | Lee, Hyung Chul Lim, Sumi Han, Jae Yong |
author_sort | Lee, Hyung Chul |
collection | PubMed |
description | Here, we investigated the role of the Wnt/β-catenin signaling pathway in chicken primordial germ cells (PGCs) in vitro. We confirmed the expression of Wnt signaling pathway-related genes and the localization of β-catenin in the nucleus, revealing that this pathway is potentially activated in chicken PGCs. Then, using the single-cell pick-up assay, we examined the proliferative capacity of cultured PGCs in response to Wnt ligands, a β-catenin-mediated Wnt signaling activator (6-bromoindirubin-3′-oxime [BIO]) or inhibitor (JW74), in the presence or absence of basic fibroblast growth factor (bFGF). WNT1, WNT3A, and BIO promoted the proliferation of chicken PGCs similarly to bFGF, whereas JW74 inhibited this proliferation. Meanwhile, such treatments in combination with bFGF did not show a synergistic effect. bFGF treatment could not rescue PGC proliferation in the presence of JW74. In addition, we confirmed the translocation of β-catenin into the nucleus by the addition of bFGF after JW74 treatment. These results indicate that there is signaling crosstalk between FGF and Wnt, and that β-catenin acts on PGC proliferation downstream of bFGF. In conclusion, our study suggests that Wnt signaling enhances the proliferation of chicken PGCs via the stabilization of β-catenin and activation of its downstream genes. |
format | Online Article Text |
id | pubmed-5062643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50626432016-10-24 Wnt/β-catenin signaling pathway activation is required for proliferation of chicken primordial germ cells in vitro Lee, Hyung Chul Lim, Sumi Han, Jae Yong Sci Rep Article Here, we investigated the role of the Wnt/β-catenin signaling pathway in chicken primordial germ cells (PGCs) in vitro. We confirmed the expression of Wnt signaling pathway-related genes and the localization of β-catenin in the nucleus, revealing that this pathway is potentially activated in chicken PGCs. Then, using the single-cell pick-up assay, we examined the proliferative capacity of cultured PGCs in response to Wnt ligands, a β-catenin-mediated Wnt signaling activator (6-bromoindirubin-3′-oxime [BIO]) or inhibitor (JW74), in the presence or absence of basic fibroblast growth factor (bFGF). WNT1, WNT3A, and BIO promoted the proliferation of chicken PGCs similarly to bFGF, whereas JW74 inhibited this proliferation. Meanwhile, such treatments in combination with bFGF did not show a synergistic effect. bFGF treatment could not rescue PGC proliferation in the presence of JW74. In addition, we confirmed the translocation of β-catenin into the nucleus by the addition of bFGF after JW74 treatment. These results indicate that there is signaling crosstalk between FGF and Wnt, and that β-catenin acts on PGC proliferation downstream of bFGF. In conclusion, our study suggests that Wnt signaling enhances the proliferation of chicken PGCs via the stabilization of β-catenin and activation of its downstream genes. Nature Publishing Group 2016-09-30 /pmc/articles/PMC5062643/ /pubmed/27687983 http://dx.doi.org/10.1038/srep34510 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Lee, Hyung Chul Lim, Sumi Han, Jae Yong Wnt/β-catenin signaling pathway activation is required for proliferation of chicken primordial germ cells in vitro |
title | Wnt/β-catenin signaling pathway activation is required for proliferation of chicken primordial germ cells in vitro |
title_full | Wnt/β-catenin signaling pathway activation is required for proliferation of chicken primordial germ cells in vitro |
title_fullStr | Wnt/β-catenin signaling pathway activation is required for proliferation of chicken primordial germ cells in vitro |
title_full_unstemmed | Wnt/β-catenin signaling pathway activation is required for proliferation of chicken primordial germ cells in vitro |
title_short | Wnt/β-catenin signaling pathway activation is required for proliferation of chicken primordial germ cells in vitro |
title_sort | wnt/β-catenin signaling pathway activation is required for proliferation of chicken primordial germ cells in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062643/ https://www.ncbi.nlm.nih.gov/pubmed/27687983 http://dx.doi.org/10.1038/srep34510 |
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