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Investigating essential gene function in Mycobacterium tuberculosis using an efficient CRISPR interference system

Despite many methodological advances that have facilitated investigation of Mycobacterium tuberculosis pathogenesis, analysis of essential gene function in this slow-growing pathogen remains difficult. Here, we describe an optimized CRISPR-based method to inhibit expression of essential genes based...

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Detalles Bibliográficos
Autores principales:	Singh, Atul K., Carette, Xavier, Potluri, Lakshmi-Prasad, Sharp, Jared D., Xu, Ranfei, Prisic, Sladjana, Husson, Robert N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062980/ https://www.ncbi.nlm.nih.gov/pubmed/27407107 http://dx.doi.org/10.1093/nar/gkw625

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