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Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections
Biofilm is an important virulence factor in Pseudomonas aeruginosa and has a substantial role in antibiotic resistance and chronic burn wound infections. New therapeutic agents against P. aeruginosa, degrading biofilms in burn wounds and improving the efficacy of current antimicrobial agents, are re...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063459/ https://www.ncbi.nlm.nih.gov/pubmed/27736961 http://dx.doi.org/10.1371/journal.pone.0164622 |
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author | Banar, Maryam Emaneini, Mohammad Satarzadeh, Mhboubeh Abdellahi, Nafiseh Beigverdi, Reza van Leeuwen, Willem B. Jabalameli, Fereshteh |
author_facet | Banar, Maryam Emaneini, Mohammad Satarzadeh, Mhboubeh Abdellahi, Nafiseh Beigverdi, Reza van Leeuwen, Willem B. Jabalameli, Fereshteh |
author_sort | Banar, Maryam |
collection | PubMed |
description | Biofilm is an important virulence factor in Pseudomonas aeruginosa and has a substantial role in antibiotic resistance and chronic burn wound infections. New therapeutic agents against P. aeruginosa, degrading biofilms in burn wounds and improving the efficacy of current antimicrobial agents, are required. In this study, the effects of α-mannosidase, β-mannosidase and trypsin enzymes on the degradation of P. aeruginosa biofilms and on the reduction of ceftazidime minimum biofilm eliminating concentrations (MBEC) were evaluated. All tested enzymes, destroyed the biofilms and reduced the ceftazidime MBECs. However, only trypsin had no cytotoxic effect on A-431 human epidermoid carcinoma cell lines. In conclusion, since trypsin had better features than mannosidase enzymes, it can be a promising agent in combatting P. aeruginosa burn wound infections. |
format | Online Article Text |
id | pubmed-5063459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50634592016-11-04 Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections Banar, Maryam Emaneini, Mohammad Satarzadeh, Mhboubeh Abdellahi, Nafiseh Beigverdi, Reza van Leeuwen, Willem B. Jabalameli, Fereshteh PLoS One Research Article Biofilm is an important virulence factor in Pseudomonas aeruginosa and has a substantial role in antibiotic resistance and chronic burn wound infections. New therapeutic agents against P. aeruginosa, degrading biofilms in burn wounds and improving the efficacy of current antimicrobial agents, are required. In this study, the effects of α-mannosidase, β-mannosidase and trypsin enzymes on the degradation of P. aeruginosa biofilms and on the reduction of ceftazidime minimum biofilm eliminating concentrations (MBEC) were evaluated. All tested enzymes, destroyed the biofilms and reduced the ceftazidime MBECs. However, only trypsin had no cytotoxic effect on A-431 human epidermoid carcinoma cell lines. In conclusion, since trypsin had better features than mannosidase enzymes, it can be a promising agent in combatting P. aeruginosa burn wound infections. Public Library of Science 2016-10-13 /pmc/articles/PMC5063459/ /pubmed/27736961 http://dx.doi.org/10.1371/journal.pone.0164622 Text en © 2016 Banar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Banar, Maryam Emaneini, Mohammad Satarzadeh, Mhboubeh Abdellahi, Nafiseh Beigverdi, Reza van Leeuwen, Willem B. Jabalameli, Fereshteh Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections |
title | Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections |
title_full | Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections |
title_fullStr | Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections |
title_full_unstemmed | Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections |
title_short | Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections |
title_sort | evaluation of mannosidase and trypsin enzymes effects on biofilm production of pseudomonas aeruginosa isolated from burn wound infections |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063459/ https://www.ncbi.nlm.nih.gov/pubmed/27736961 http://dx.doi.org/10.1371/journal.pone.0164622 |
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