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Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections

Biofilm is an important virulence factor in Pseudomonas aeruginosa and has a substantial role in antibiotic resistance and chronic burn wound infections. New therapeutic agents against P. aeruginosa, degrading biofilms in burn wounds and improving the efficacy of current antimicrobial agents, are re...

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Autores principales: Banar, Maryam, Emaneini, Mohammad, Satarzadeh, Mhboubeh, Abdellahi, Nafiseh, Beigverdi, Reza, van Leeuwen, Willem B., Jabalameli, Fereshteh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063459/
https://www.ncbi.nlm.nih.gov/pubmed/27736961
http://dx.doi.org/10.1371/journal.pone.0164622
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author Banar, Maryam
Emaneini, Mohammad
Satarzadeh, Mhboubeh
Abdellahi, Nafiseh
Beigverdi, Reza
van Leeuwen, Willem B.
Jabalameli, Fereshteh
author_facet Banar, Maryam
Emaneini, Mohammad
Satarzadeh, Mhboubeh
Abdellahi, Nafiseh
Beigverdi, Reza
van Leeuwen, Willem B.
Jabalameli, Fereshteh
author_sort Banar, Maryam
collection PubMed
description Biofilm is an important virulence factor in Pseudomonas aeruginosa and has a substantial role in antibiotic resistance and chronic burn wound infections. New therapeutic agents against P. aeruginosa, degrading biofilms in burn wounds and improving the efficacy of current antimicrobial agents, are required. In this study, the effects of α-mannosidase, β-mannosidase and trypsin enzymes on the degradation of P. aeruginosa biofilms and on the reduction of ceftazidime minimum biofilm eliminating concentrations (MBEC) were evaluated. All tested enzymes, destroyed the biofilms and reduced the ceftazidime MBECs. However, only trypsin had no cytotoxic effect on A-431 human epidermoid carcinoma cell lines. In conclusion, since trypsin had better features than mannosidase enzymes, it can be a promising agent in combatting P. aeruginosa burn wound infections.
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spelling pubmed-50634592016-11-04 Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections Banar, Maryam Emaneini, Mohammad Satarzadeh, Mhboubeh Abdellahi, Nafiseh Beigverdi, Reza van Leeuwen, Willem B. Jabalameli, Fereshteh PLoS One Research Article Biofilm is an important virulence factor in Pseudomonas aeruginosa and has a substantial role in antibiotic resistance and chronic burn wound infections. New therapeutic agents against P. aeruginosa, degrading biofilms in burn wounds and improving the efficacy of current antimicrobial agents, are required. In this study, the effects of α-mannosidase, β-mannosidase and trypsin enzymes on the degradation of P. aeruginosa biofilms and on the reduction of ceftazidime minimum biofilm eliminating concentrations (MBEC) were evaluated. All tested enzymes, destroyed the biofilms and reduced the ceftazidime MBECs. However, only trypsin had no cytotoxic effect on A-431 human epidermoid carcinoma cell lines. In conclusion, since trypsin had better features than mannosidase enzymes, it can be a promising agent in combatting P. aeruginosa burn wound infections. Public Library of Science 2016-10-13 /pmc/articles/PMC5063459/ /pubmed/27736961 http://dx.doi.org/10.1371/journal.pone.0164622 Text en © 2016 Banar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Banar, Maryam
Emaneini, Mohammad
Satarzadeh, Mhboubeh
Abdellahi, Nafiseh
Beigverdi, Reza
van Leeuwen, Willem B.
Jabalameli, Fereshteh
Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections
title Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections
title_full Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections
title_fullStr Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections
title_full_unstemmed Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections
title_short Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections
title_sort evaluation of mannosidase and trypsin enzymes effects on biofilm production of pseudomonas aeruginosa isolated from burn wound infections
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063459/
https://www.ncbi.nlm.nih.gov/pubmed/27736961
http://dx.doi.org/10.1371/journal.pone.0164622
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