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Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells

The spatial and temporal control of microtubule dynamics is fundamentally important for proper spindle assembly and chromosome segregation. This is achieved, in part, by the multitude of proteins that bind to and regulate spindle microtubules, including kinesin superfamily members, which act as micr...

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Autores principales: Walczak, Claire E., Zong, Hailing, Jain, Sachin, Stout, Jane R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063611/
https://www.ncbi.nlm.nih.gov/pubmed/27559136
http://dx.doi.org/10.1091/mbc.E16-04-0254
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author Walczak, Claire E.
Zong, Hailing
Jain, Sachin
Stout, Jane R.
author_facet Walczak, Claire E.
Zong, Hailing
Jain, Sachin
Stout, Jane R.
author_sort Walczak, Claire E.
collection PubMed
description The spatial and temporal control of microtubule dynamics is fundamentally important for proper spindle assembly and chromosome segregation. This is achieved, in part, by the multitude of proteins that bind to and regulate spindle microtubules, including kinesin superfamily members, which act as microtubule-destabilizing enzymes. These fall into two general classes: the kinesin-13 proteins, which directly depolymerize microtubules, and the kinesin-8 proteins, which are plus end–directed motors that either destabilize microtubules or cap the microtubule plus ends. Here we analyze the contribution of a PtK kinesin-8 protein, Kif18B, in the control of mitotic microtubule dynamics. Knockdown of Kif18B causes defects in spindle microtubule organization and a dramatic increase in astral microtubules. Kif18B-knockdown cells had defects in chromosome alignment, but there were no defects in chromosome segregation. The long astral microtubules that occur in the absence of Kif18B are limited in length by the cell cortex. Using EB1 tracking, we show that Kif18B activity is spatially controlled, as loss of Kif18B has the most dramatic effect on the lifetimes of astral microtubules that extend toward the cell cortex. Together our studies provide new insight into how diverse kinesins contribute to spatial microtubule organization in the spindle.
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spelling pubmed-50636112016-12-30 Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells Walczak, Claire E. Zong, Hailing Jain, Sachin Stout, Jane R. Mol Biol Cell Brief Reports The spatial and temporal control of microtubule dynamics is fundamentally important for proper spindle assembly and chromosome segregation. This is achieved, in part, by the multitude of proteins that bind to and regulate spindle microtubules, including kinesin superfamily members, which act as microtubule-destabilizing enzymes. These fall into two general classes: the kinesin-13 proteins, which directly depolymerize microtubules, and the kinesin-8 proteins, which are plus end–directed motors that either destabilize microtubules or cap the microtubule plus ends. Here we analyze the contribution of a PtK kinesin-8 protein, Kif18B, in the control of mitotic microtubule dynamics. Knockdown of Kif18B causes defects in spindle microtubule organization and a dramatic increase in astral microtubules. Kif18B-knockdown cells had defects in chromosome alignment, but there were no defects in chromosome segregation. The long astral microtubules that occur in the absence of Kif18B are limited in length by the cell cortex. Using EB1 tracking, we show that Kif18B activity is spatially controlled, as loss of Kif18B has the most dramatic effect on the lifetimes of astral microtubules that extend toward the cell cortex. Together our studies provide new insight into how diverse kinesins contribute to spatial microtubule organization in the spindle. The American Society for Cell Biology 2016-10-15 /pmc/articles/PMC5063611/ /pubmed/27559136 http://dx.doi.org/10.1091/mbc.E16-04-0254 Text en © 2016 Walczak et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Brief Reports
Walczak, Claire E.
Zong, Hailing
Jain, Sachin
Stout, Jane R.
Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
title Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
title_full Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
title_fullStr Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
title_full_unstemmed Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
title_short Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
title_sort spatial regulation of astral microtubule dynamics by kif18b in ptk cells
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063611/
https://www.ncbi.nlm.nih.gov/pubmed/27559136
http://dx.doi.org/10.1091/mbc.E16-04-0254
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