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Conformational plasticity of JRAB/MICAL-L2 provides “law and order” in collective cell migration

In fundamental biological processes, cells often move in groups, a process termed collective cell migration. Collectively migrating cells are much better organized than a random assemblage of individual cells. Many molecules have been identified as factors involved in collective cell migration, and...

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Autores principales: Sakane, Ayuko, Yoshizawa, Shin, Nishimura, Masaomi, Tsuchiya, Yuko, Matsushita, Natsuki, Miyake, Kazuhisa, Horikawa, Kazuki, Imoto, Issei, Mizuguchi, Chiharu, Saito, Hiroyuki, Ueno, Takato, Matsushita, Sachi, Haga, Hisashi, Deguchi, Shinji, Mizuguchi, Kenji, Yokota, Hideo, Sasaki, Takuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063617/
https://www.ncbi.nlm.nih.gov/pubmed/27582384
http://dx.doi.org/10.1091/mbc.E16-05-0332
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author Sakane, Ayuko
Yoshizawa, Shin
Nishimura, Masaomi
Tsuchiya, Yuko
Matsushita, Natsuki
Miyake, Kazuhisa
Horikawa, Kazuki
Imoto, Issei
Mizuguchi, Chiharu
Saito, Hiroyuki
Ueno, Takato
Matsushita, Sachi
Haga, Hisashi
Deguchi, Shinji
Mizuguchi, Kenji
Yokota, Hideo
Sasaki, Takuya
author_facet Sakane, Ayuko
Yoshizawa, Shin
Nishimura, Masaomi
Tsuchiya, Yuko
Matsushita, Natsuki
Miyake, Kazuhisa
Horikawa, Kazuki
Imoto, Issei
Mizuguchi, Chiharu
Saito, Hiroyuki
Ueno, Takato
Matsushita, Sachi
Haga, Hisashi
Deguchi, Shinji
Mizuguchi, Kenji
Yokota, Hideo
Sasaki, Takuya
author_sort Sakane, Ayuko
collection PubMed
description In fundamental biological processes, cells often move in groups, a process termed collective cell migration. Collectively migrating cells are much better organized than a random assemblage of individual cells. Many molecules have been identified as factors involved in collective cell migration, and no one molecule is adequate to explain the whole picture. Here we show that JRAB/MICAL-L2, an effector protein of Rab13 GTPase, provides the “law and order” allowing myriad cells to behave as a single unit just by changing its conformation. First, we generated a structural model of JRAB/MICAL-L2 by a combination of bioinformatic and biochemical analyses and showed how JRAB/MICAL-L2 interacts with Rab13 and how its conformational change occurs. We combined cell biology, live imaging, computational biology, and biomechanics to show that impairment of conformational plasticity in JRAB/MICAL-L2 causes excessive rigidity and loss of directionality, leading to imbalance in cell group behavior. This multidisciplinary approach supports the concept that the conformational plasticity of a single molecule provides “law and order” in collective cell migration.
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spelling pubmed-50636172016-12-30 Conformational plasticity of JRAB/MICAL-L2 provides “law and order” in collective cell migration Sakane, Ayuko Yoshizawa, Shin Nishimura, Masaomi Tsuchiya, Yuko Matsushita, Natsuki Miyake, Kazuhisa Horikawa, Kazuki Imoto, Issei Mizuguchi, Chiharu Saito, Hiroyuki Ueno, Takato Matsushita, Sachi Haga, Hisashi Deguchi, Shinji Mizuguchi, Kenji Yokota, Hideo Sasaki, Takuya Mol Biol Cell Articles In fundamental biological processes, cells often move in groups, a process termed collective cell migration. Collectively migrating cells are much better organized than a random assemblage of individual cells. Many molecules have been identified as factors involved in collective cell migration, and no one molecule is adequate to explain the whole picture. Here we show that JRAB/MICAL-L2, an effector protein of Rab13 GTPase, provides the “law and order” allowing myriad cells to behave as a single unit just by changing its conformation. First, we generated a structural model of JRAB/MICAL-L2 by a combination of bioinformatic and biochemical analyses and showed how JRAB/MICAL-L2 interacts with Rab13 and how its conformational change occurs. We combined cell biology, live imaging, computational biology, and biomechanics to show that impairment of conformational plasticity in JRAB/MICAL-L2 causes excessive rigidity and loss of directionality, leading to imbalance in cell group behavior. This multidisciplinary approach supports the concept that the conformational plasticity of a single molecule provides “law and order” in collective cell migration. The American Society for Cell Biology 2016-10-15 /pmc/articles/PMC5063617/ /pubmed/27582384 http://dx.doi.org/10.1091/mbc.E16-05-0332 Text en © 2016 Sakane et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Sakane, Ayuko
Yoshizawa, Shin
Nishimura, Masaomi
Tsuchiya, Yuko
Matsushita, Natsuki
Miyake, Kazuhisa
Horikawa, Kazuki
Imoto, Issei
Mizuguchi, Chiharu
Saito, Hiroyuki
Ueno, Takato
Matsushita, Sachi
Haga, Hisashi
Deguchi, Shinji
Mizuguchi, Kenji
Yokota, Hideo
Sasaki, Takuya
Conformational plasticity of JRAB/MICAL-L2 provides “law and order” in collective cell migration
title Conformational plasticity of JRAB/MICAL-L2 provides “law and order” in collective cell migration
title_full Conformational plasticity of JRAB/MICAL-L2 provides “law and order” in collective cell migration
title_fullStr Conformational plasticity of JRAB/MICAL-L2 provides “law and order” in collective cell migration
title_full_unstemmed Conformational plasticity of JRAB/MICAL-L2 provides “law and order” in collective cell migration
title_short Conformational plasticity of JRAB/MICAL-L2 provides “law and order” in collective cell migration
title_sort conformational plasticity of jrab/mical-l2 provides “law and order” in collective cell migration
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063617/
https://www.ncbi.nlm.nih.gov/pubmed/27582384
http://dx.doi.org/10.1091/mbc.E16-05-0332
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