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Application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy

Background and study aims: Biliary stent dysfunction is mainly caused by biliary sludge that forms as a result of bacterial adherence and subsequent biofilm formation on the inner surface of the stent. Silver ions arewell known to have excellent antimicrobial activity against a wide range of microor...

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Autores principales: Yamabe, Akane, Irisawa, Atsushi, Wada, Ikuo, Shibukawa, Goro, Fujisawa, Mariko, Sato, Ai, Igarashi, Ryo, Maki, Takumi, Hoshi, Koki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: © Georg Thieme Verlag KG 2016
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063737/
https://www.ncbi.nlm.nih.gov/pubmed/27747284
http://dx.doi.org/10.1055/s-0042-115173
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author Yamabe, Akane
Irisawa, Atsushi
Wada, Ikuo
Shibukawa, Goro
Fujisawa, Mariko
Sato, Ai
Igarashi, Ryo
Maki, Takumi
Hoshi, Koki
author_facet Yamabe, Akane
Irisawa, Atsushi
Wada, Ikuo
Shibukawa, Goro
Fujisawa, Mariko
Sato, Ai
Igarashi, Ryo
Maki, Takumi
Hoshi, Koki
author_sort Yamabe, Akane
collection PubMed
description Background and study aims: Biliary stent dysfunction is mainly caused by biliary sludge that forms as a result of bacterial adherence and subsequent biofilm formation on the inner surface of the stent. Silver ions arewell known to have excellent antimicrobial activity against a wide range of microorganisms. In this study, we designed and constructed silver-coated plastic stent (PS) and investigated whether the silver coating prevented bacterial adherence and biofilm formation through the use of electron microscopy. Material and methods: The polyurethane PS with/without silver coating were prepared in 6-inch segments. The silver-based antimicrobial agents were electrostatically applied onto the stent surface. The stents were then immersed for 5 weeks in infected human bile juice obtained from a patient with cholangitis, and electron microscopy was used to investigate the ability of the modified PS to prevent bacterial adherence and biofilm formation. Results: The bacterial flora did not change before and after immersion of stents in both the group with and without silver coating. Electron microscopic observation revealed meshwork-like structures around the bacteria, characteristic of biofilm-forming bacteria, in all stents from the control group (6/6, 100 %). On the other hand, a limited number of bacteria were observed in all stents in the silver-coated group, and no apparent biofilm formation was observed (0/6, 0 %). Conclusions: The significance of the findings from our study is the ability of silver-coated PS to prevent biofilm formation on the stent surface, which results in the prevention of stent occlusion.
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spelling pubmed-50637372016-10-14 Application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy Yamabe, Akane Irisawa, Atsushi Wada, Ikuo Shibukawa, Goro Fujisawa, Mariko Sato, Ai Igarashi, Ryo Maki, Takumi Hoshi, Koki Endosc Int Open Background and study aims: Biliary stent dysfunction is mainly caused by biliary sludge that forms as a result of bacterial adherence and subsequent biofilm formation on the inner surface of the stent. Silver ions arewell known to have excellent antimicrobial activity against a wide range of microorganisms. In this study, we designed and constructed silver-coated plastic stent (PS) and investigated whether the silver coating prevented bacterial adherence and biofilm formation through the use of electron microscopy. Material and methods: The polyurethane PS with/without silver coating were prepared in 6-inch segments. The silver-based antimicrobial agents were electrostatically applied onto the stent surface. The stents were then immersed for 5 weeks in infected human bile juice obtained from a patient with cholangitis, and electron microscopy was used to investigate the ability of the modified PS to prevent bacterial adherence and biofilm formation. Results: The bacterial flora did not change before and after immersion of stents in both the group with and without silver coating. Electron microscopic observation revealed meshwork-like structures around the bacteria, characteristic of biofilm-forming bacteria, in all stents from the control group (6/6, 100 %). On the other hand, a limited number of bacteria were observed in all stents in the silver-coated group, and no apparent biofilm formation was observed (0/6, 0 %). Conclusions: The significance of the findings from our study is the ability of silver-coated PS to prevent biofilm formation on the stent surface, which results in the prevention of stent occlusion. © Georg Thieme Verlag KG 2016-10 2016-09-14 /pmc/articles/PMC5063737/ /pubmed/27747284 http://dx.doi.org/10.1055/s-0042-115173 Text en © Thieme Medical Publishers
spellingShingle Yamabe, Akane
Irisawa, Atsushi
Wada, Ikuo
Shibukawa, Goro
Fujisawa, Mariko
Sato, Ai
Igarashi, Ryo
Maki, Takumi
Hoshi, Koki
Application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy
title Application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy
title_full Application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy
title_fullStr Application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy
title_full_unstemmed Application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy
title_short Application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy
title_sort application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063737/
https://www.ncbi.nlm.nih.gov/pubmed/27747284
http://dx.doi.org/10.1055/s-0042-115173
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