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A High-Yield Two-Hour Protocol for Extraction of Human Hair Shaft Proteins
Proteome analysis of the human hair remains challenging due to the poor solubility of hair proteins and the difficulty in their extraction. In the present study, we have developed a rapid extraction protocol for hair shaft protein using alkaline-based buffer. The new protocol accelerated the procedu...
Autores principales: | Wong, Sing Ying, Lee, Ching Chin, Ashrafzadeh, Ali, Junit, Sarni Mat, Abrahim, Nazirahanie, Hashim, Onn Haji |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5065217/ https://www.ncbi.nlm.nih.gov/pubmed/27741315 http://dx.doi.org/10.1371/journal.pone.0164993 |
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