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The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis
Bacterial growth of peritoneal fluid specimens obtained during surgical procedures for acute appendicitis may be useful to optimize further antibiotic therapy in complicated cases. DNA amplification represents a fast technique to detect microbial sequences. We aimed to compare the potential of DNA a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PAGEPress Publications, Pavia, Italy
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066096/ https://www.ncbi.nlm.nih.gov/pubmed/27777701 http://dx.doi.org/10.4081/pr.2016.6487 |
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author | Tocchioni, Francesca Tani, Chiara Bartolini, Laura Moriondo, Maria Nieddu, Francesco Pecile, Patrizia Azzari, Chiara Messineo, Antonio Ghionzoli, Marco |
author_facet | Tocchioni, Francesca Tani, Chiara Bartolini, Laura Moriondo, Maria Nieddu, Francesco Pecile, Patrizia Azzari, Chiara Messineo, Antonio Ghionzoli, Marco |
author_sort | Tocchioni, Francesca |
collection | PubMed |
description | Bacterial growth of peritoneal fluid specimens obtained during surgical procedures for acute appendicitis may be useful to optimize further antibiotic therapy in complicated cases. DNA amplification represents a fast technique to detect microbial sequences. We aimed to compare the potential of DNA amplification versus traditional bacterial growth culture highlighting advantages and drawbacks in a surgical setting. Peritoneal fluid specimens were collected during surgery from 36 children who underwent appendectomy between May and December 2012. Real-time polymerase chain reaction (RT-PCR) and cultures were performed on each sample. RT-PCR showed an amplification of 16S in 18/36 samples, Escherichia coli (in 7 cases), Pseudomonas aeruginosa (3), Fusobacterium necrophorum (3), Adenovirus (2), E.coli (1), Klebsiella pneumoniae (1), Serratia marcescens/Enterobacter cloacae (1). Bacterial growth was instead observed only in four patients (3 E.coli and 1 P.aeruginosa and Bacteroides ovatus). Preoperative C-reactive protein and inflammation degree, the most reliable indicators of bacterial translocation, were elevated as expected. DNA amplification was a quick and useful method to detect pathogens and it was even more valuable in detecting aggressive pathogens such as anaerobes, difficult to preserve in biological cultures; its drawbacks were the lack of biological growths and of antibiograms. In our pilot study RT-PCR and cultures did not influence the way patients were treated. |
format | Online Article Text |
id | pubmed-5066096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | PAGEPress Publications, Pavia, Italy |
record_format | MEDLINE/PubMed |
spelling | pubmed-50660962016-10-24 The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis Tocchioni, Francesca Tani, Chiara Bartolini, Laura Moriondo, Maria Nieddu, Francesco Pecile, Patrizia Azzari, Chiara Messineo, Antonio Ghionzoli, Marco Pediatr Rep Article Bacterial growth of peritoneal fluid specimens obtained during surgical procedures for acute appendicitis may be useful to optimize further antibiotic therapy in complicated cases. DNA amplification represents a fast technique to detect microbial sequences. We aimed to compare the potential of DNA amplification versus traditional bacterial growth culture highlighting advantages and drawbacks in a surgical setting. Peritoneal fluid specimens were collected during surgery from 36 children who underwent appendectomy between May and December 2012. Real-time polymerase chain reaction (RT-PCR) and cultures were performed on each sample. RT-PCR showed an amplification of 16S in 18/36 samples, Escherichia coli (in 7 cases), Pseudomonas aeruginosa (3), Fusobacterium necrophorum (3), Adenovirus (2), E.coli (1), Klebsiella pneumoniae (1), Serratia marcescens/Enterobacter cloacae (1). Bacterial growth was instead observed only in four patients (3 E.coli and 1 P.aeruginosa and Bacteroides ovatus). Preoperative C-reactive protein and inflammation degree, the most reliable indicators of bacterial translocation, were elevated as expected. DNA amplification was a quick and useful method to detect pathogens and it was even more valuable in detecting aggressive pathogens such as anaerobes, difficult to preserve in biological cultures; its drawbacks were the lack of biological growths and of antibiograms. In our pilot study RT-PCR and cultures did not influence the way patients were treated. PAGEPress Publications, Pavia, Italy 2016-09-19 /pmc/articles/PMC5066096/ /pubmed/27777701 http://dx.doi.org/10.4081/pr.2016.6487 Text en ©Copyright F. Tocchioni et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Tocchioni, Francesca Tani, Chiara Bartolini, Laura Moriondo, Maria Nieddu, Francesco Pecile, Patrizia Azzari, Chiara Messineo, Antonio Ghionzoli, Marco The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis |
title | The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis |
title_full | The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis |
title_fullStr | The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis |
title_full_unstemmed | The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis |
title_short | The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis |
title_sort | role of dna amplification and cultural growth in complicated acute appendicitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066096/ https://www.ncbi.nlm.nih.gov/pubmed/27777701 http://dx.doi.org/10.4081/pr.2016.6487 |
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