Cargando…
Haplotype hitchhiking promotes trait coselection in Brassica napus
Local haplotype patterns surrounding densely spaced DNA markers with significant trait associations can reveal information on selective sweeps and genome diversity associated with important crop traits. Relationships between haplotype and phenotype diversity, coupled with analysis of gene content in...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066645/ https://www.ncbi.nlm.nih.gov/pubmed/26800855 http://dx.doi.org/10.1111/pbi.12521 |
_version_ | 1782460521710092288 |
---|---|
author | Qian, Lunwen Qian, Wei Snowdon, Rod J. |
author_facet | Qian, Lunwen Qian, Wei Snowdon, Rod J. |
author_sort | Qian, Lunwen |
collection | PubMed |
description | Local haplotype patterns surrounding densely spaced DNA markers with significant trait associations can reveal information on selective sweeps and genome diversity associated with important crop traits. Relationships between haplotype and phenotype diversity, coupled with analysis of gene content in conserved haplotype blocks, can provide insight into coselection for nonrelated traits. We performed genome‐wide analysis of haplotypes associated with the important physiological and agronomic traits leaf chlorophyll and seed glucosinolate content, respectively, in the major oilseed crop species Brassica napus. A locus on chromosome A01 showed opposite effects on leaf chlorophyll content and seed glucosinolate content, attributed to strong linkage disequilibrium (LD) between orthologues of the chlorophyll biosynthesis genes EARLY LIGHT‐INDUCED PROTEIN and CHLOROPHYLL SYNTHASE, and the glucosinolate synthesis gene ATP SULFURYLASE 1. Another conserved haplotype block, on chromosome A02, contained a number of chlorophyll‐related genes in LD with orthologues of the key glucosinolate biosynthesis genes METHYLTHIOALKYMALATE SYNTHASE‐LIKE 1 and 3. Multigene haplogroups were found to have a significantly greater contribution to variation for chlorophyll content than haplotypes for any single gene, suggesting positive effects of additive locus accumulation. Detailed reanalysis of population substructure revealed a clade of ten related accessions exhibiting high leaf chlorophyll and low seed glucosinolate content. These accessions each carried one of the above‐mentioned haplotypes from A01 or A02, generally in combination with further chlorophyll‐associated haplotypes from chromosomes A05 and/or C05. The phenotypic rather than pleiotropic correlations between leaf chlorophyll content index and seed GSL suggest that LD may have led to inadvertent coselection for these two traits. |
format | Online Article Text |
id | pubmed-5066645 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50666452016-11-01 Haplotype hitchhiking promotes trait coselection in Brassica napus Qian, Lunwen Qian, Wei Snowdon, Rod J. Plant Biotechnol J Research Articles Local haplotype patterns surrounding densely spaced DNA markers with significant trait associations can reveal information on selective sweeps and genome diversity associated with important crop traits. Relationships between haplotype and phenotype diversity, coupled with analysis of gene content in conserved haplotype blocks, can provide insight into coselection for nonrelated traits. We performed genome‐wide analysis of haplotypes associated with the important physiological and agronomic traits leaf chlorophyll and seed glucosinolate content, respectively, in the major oilseed crop species Brassica napus. A locus on chromosome A01 showed opposite effects on leaf chlorophyll content and seed glucosinolate content, attributed to strong linkage disequilibrium (LD) between orthologues of the chlorophyll biosynthesis genes EARLY LIGHT‐INDUCED PROTEIN and CHLOROPHYLL SYNTHASE, and the glucosinolate synthesis gene ATP SULFURYLASE 1. Another conserved haplotype block, on chromosome A02, contained a number of chlorophyll‐related genes in LD with orthologues of the key glucosinolate biosynthesis genes METHYLTHIOALKYMALATE SYNTHASE‐LIKE 1 and 3. Multigene haplogroups were found to have a significantly greater contribution to variation for chlorophyll content than haplotypes for any single gene, suggesting positive effects of additive locus accumulation. Detailed reanalysis of population substructure revealed a clade of ten related accessions exhibiting high leaf chlorophyll and low seed glucosinolate content. These accessions each carried one of the above‐mentioned haplotypes from A01 or A02, generally in combination with further chlorophyll‐associated haplotypes from chromosomes A05 and/or C05. The phenotypic rather than pleiotropic correlations between leaf chlorophyll content index and seed GSL suggest that LD may have led to inadvertent coselection for these two traits. John Wiley and Sons Inc. 2016-01-23 2016-07 /pmc/articles/PMC5066645/ /pubmed/26800855 http://dx.doi.org/10.1111/pbi.12521 Text en © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Qian, Lunwen Qian, Wei Snowdon, Rod J. Haplotype hitchhiking promotes trait coselection in Brassica napus |
title | Haplotype hitchhiking promotes trait coselection in Brassica napus
|
title_full | Haplotype hitchhiking promotes trait coselection in Brassica napus
|
title_fullStr | Haplotype hitchhiking promotes trait coselection in Brassica napus
|
title_full_unstemmed | Haplotype hitchhiking promotes trait coselection in Brassica napus
|
title_short | Haplotype hitchhiking promotes trait coselection in Brassica napus
|
title_sort | haplotype hitchhiking promotes trait coselection in brassica napus |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066645/ https://www.ncbi.nlm.nih.gov/pubmed/26800855 http://dx.doi.org/10.1111/pbi.12521 |
work_keys_str_mv | AT qianlunwen haplotypehitchhikingpromotestraitcoselectioninbrassicanapus AT qianwei haplotypehitchhikingpromotestraitcoselectioninbrassicanapus AT snowdonrodj haplotypehitchhikingpromotestraitcoselectioninbrassicanapus |