Magnet-Bead Based MicroRNA Delivery System to Modify CD133(+) Stem Cells

Aim. CD133(+) stem cells bear huge potential for regenerative medicine. However, low retention in the injured tissue and massive cell death reduce beneficial effects. In order to address these issues, we intended to develop a nonviral system for appropriate cell engineering. Materials and Methods. M...

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Detalles Bibliográficos
Autores principales: Müller, Paula, Voronina, Natalia, Hausburg, Frauke, Lux, Cornelia A., Wiekhorst, Frank, Steinhoff, Gustav, David, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067480/
https://www.ncbi.nlm.nih.gov/pubmed/27795713
http://dx.doi.org/10.1155/2016/7152761
Descripción
Sumario:Aim. CD133(+) stem cells bear huge potential for regenerative medicine. However, low retention in the injured tissue and massive cell death reduce beneficial effects. In order to address these issues, we intended to develop a nonviral system for appropriate cell engineering. Materials and Methods. Modification of human CD133(+) stem cells with magnetic polyplexes carrying microRNA was studied in terms of efficiency, safety, and targeting potential. Results. High microRNA uptake rates (~80–90%) were achieved without affecting CD133(+) stem cell properties. Modified cells can be magnetically guided. Conclusion. We developed a safe and efficient protocol for CD133(+) stem cell modification. Our work may become a basis to improve stem cell therapeutical effects as well as their monitoring with magnetic resonance imaging.

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