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Genome-wide analysis of alternative splicing during human heart development
Alternative splicing (AS) drives determinative changes during mouse heart development. Recent high-throughput technological advancements have facilitated genome-wide AS, while its analysis in human foetal heart transition to the adult stage has not been reported. Here, we present a high-resolution g...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067579/ https://www.ncbi.nlm.nih.gov/pubmed/27752099 http://dx.doi.org/10.1038/srep35520 |
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author | Wang, He Chen, Yanmei Li, Xinzhong Chen, Guojun Zhong, Lintao Chen, Gangbing Liao, Yulin Liao, Wangjun Bin, Jianping |
author_facet | Wang, He Chen, Yanmei Li, Xinzhong Chen, Guojun Zhong, Lintao Chen, Gangbing Liao, Yulin Liao, Wangjun Bin, Jianping |
author_sort | Wang, He |
collection | PubMed |
description | Alternative splicing (AS) drives determinative changes during mouse heart development. Recent high-throughput technological advancements have facilitated genome-wide AS, while its analysis in human foetal heart transition to the adult stage has not been reported. Here, we present a high-resolution global analysis of AS transitions between human foetal and adult hearts. RNA-sequencing data showed extensive AS transitions occurred between human foetal and adult hearts, and AS events occurred more frequently in protein-coding genes than in long non-coding RNA (lncRNA). A significant difference of AS patterns was found between foetal and adult hearts. The predicted difference in AS events was further confirmed using quantitative reverse transcription-polymerase chain reaction analysis of human heart samples. Functional foetal-specific AS event analysis showed enrichment associated with cell proliferation-related pathways including cell cycle, whereas adult-specific AS events were associated with protein synthesis. Furthermore, 42.6% of foetal-specific AS events showed significant changes in gene expression levels between foetal and adult hearts. Genes exhibiting both foetal-specific AS and differential expression were highly enriched in cell cycle-associated functions. In conclusion, we provided a genome-wide profiling of AS transitions between foetal and adult hearts and proposed that AS transitions and deferential gene expression may play determinative roles in human heart development. |
format | Online Article Text |
id | pubmed-5067579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50675792016-10-26 Genome-wide analysis of alternative splicing during human heart development Wang, He Chen, Yanmei Li, Xinzhong Chen, Guojun Zhong, Lintao Chen, Gangbing Liao, Yulin Liao, Wangjun Bin, Jianping Sci Rep Article Alternative splicing (AS) drives determinative changes during mouse heart development. Recent high-throughput technological advancements have facilitated genome-wide AS, while its analysis in human foetal heart transition to the adult stage has not been reported. Here, we present a high-resolution global analysis of AS transitions between human foetal and adult hearts. RNA-sequencing data showed extensive AS transitions occurred between human foetal and adult hearts, and AS events occurred more frequently in protein-coding genes than in long non-coding RNA (lncRNA). A significant difference of AS patterns was found between foetal and adult hearts. The predicted difference in AS events was further confirmed using quantitative reverse transcription-polymerase chain reaction analysis of human heart samples. Functional foetal-specific AS event analysis showed enrichment associated with cell proliferation-related pathways including cell cycle, whereas adult-specific AS events were associated with protein synthesis. Furthermore, 42.6% of foetal-specific AS events showed significant changes in gene expression levels between foetal and adult hearts. Genes exhibiting both foetal-specific AS and differential expression were highly enriched in cell cycle-associated functions. In conclusion, we provided a genome-wide profiling of AS transitions between foetal and adult hearts and proposed that AS transitions and deferential gene expression may play determinative roles in human heart development. Nature Publishing Group 2016-10-18 /pmc/articles/PMC5067579/ /pubmed/27752099 http://dx.doi.org/10.1038/srep35520 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wang, He Chen, Yanmei Li, Xinzhong Chen, Guojun Zhong, Lintao Chen, Gangbing Liao, Yulin Liao, Wangjun Bin, Jianping Genome-wide analysis of alternative splicing during human heart development |
title | Genome-wide analysis of alternative splicing during human heart development |
title_full | Genome-wide analysis of alternative splicing during human heart development |
title_fullStr | Genome-wide analysis of alternative splicing during human heart development |
title_full_unstemmed | Genome-wide analysis of alternative splicing during human heart development |
title_short | Genome-wide analysis of alternative splicing during human heart development |
title_sort | genome-wide analysis of alternative splicing during human heart development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067579/ https://www.ncbi.nlm.nih.gov/pubmed/27752099 http://dx.doi.org/10.1038/srep35520 |
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