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Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates
This study aimed to assess the antibacterial and antibiotic potentiating property of Vangueria madagascariensis (VM) (fruit and leaf extracts) against 10 clinical isolates. A microdilution broth susceptibility assay for bacteria was used for the determination of the minimum inhibitory concentration...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067847/ https://www.ncbi.nlm.nih.gov/pubmed/27774426 http://dx.doi.org/10.1016/j.jtcme.2015.09.002 |
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author | Mahomoodally, M. Fawzi Dilmohamed, Schajeed |
author_facet | Mahomoodally, M. Fawzi Dilmohamed, Schajeed |
author_sort | Mahomoodally, M. Fawzi |
collection | PubMed |
description | This study aimed to assess the antibacterial and antibiotic potentiating property of Vangueria madagascariensis (VM) (fruit and leaf extracts) against 10 clinical isolates. A microdilution broth susceptibility assay for bacteria was used for the determination of the minimum inhibitory concentration (MIC) and associated with antibiotics to evaluate any synergistic effect. VM extracts were found to potentiate the activity of 3 conventional antibiotics. Chloramphenicol and Ciprofloxacin showed no activity against Acinetobacter spp. but when mixed with VM (in a ratio of 50% VM extracts: 30% antibiotic), showed potentiating effect. The methanolic fruit extract at lower concentration of Chloramphenicol (30%) gave better synergistic effect (MIC = 3.75 μg/mL) as compared to 50% (MIC = 12.5 μg/mL). With Gentamicin, no activity was detected with leaf decoction but other extracts (methanolic leaf/fruit extract and fruit decoction) showed enhancement (MIC- 0.47, 7.5 and 15 μg/mL respectively). Interestingly, Chloramphenicol showed no activity against MRSA, but when mixed with VM, produced low MICs (<0.39–0.78 μg/mL with 50% antibiotic and from <0.47 to 0.94 μg/mL with 30% antibiotic). Combining Gentamicin with VM extracts showed an enhancement in the potentiating activity against MRSA. In conclusion, the observed antimicrobial property of VM tend to suggest a promising alternative and complementary strategy to manage bacterial infections and hence can open new avenues for further research using traditional medicinal food plant. |
format | Online Article Text |
id | pubmed-5067847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-50678472016-10-21 Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates Mahomoodally, M. Fawzi Dilmohamed, Schajeed J Tradit Complement Med Original Article This study aimed to assess the antibacterial and antibiotic potentiating property of Vangueria madagascariensis (VM) (fruit and leaf extracts) against 10 clinical isolates. A microdilution broth susceptibility assay for bacteria was used for the determination of the minimum inhibitory concentration (MIC) and associated with antibiotics to evaluate any synergistic effect. VM extracts were found to potentiate the activity of 3 conventional antibiotics. Chloramphenicol and Ciprofloxacin showed no activity against Acinetobacter spp. but when mixed with VM (in a ratio of 50% VM extracts: 30% antibiotic), showed potentiating effect. The methanolic fruit extract at lower concentration of Chloramphenicol (30%) gave better synergistic effect (MIC = 3.75 μg/mL) as compared to 50% (MIC = 12.5 μg/mL). With Gentamicin, no activity was detected with leaf decoction but other extracts (methanolic leaf/fruit extract and fruit decoction) showed enhancement (MIC- 0.47, 7.5 and 15 μg/mL respectively). Interestingly, Chloramphenicol showed no activity against MRSA, but when mixed with VM, produced low MICs (<0.39–0.78 μg/mL with 50% antibiotic and from <0.47 to 0.94 μg/mL with 30% antibiotic). Combining Gentamicin with VM extracts showed an enhancement in the potentiating activity against MRSA. In conclusion, the observed antimicrobial property of VM tend to suggest a promising alternative and complementary strategy to manage bacterial infections and hence can open new avenues for further research using traditional medicinal food plant. Elsevier 2015-11-14 /pmc/articles/PMC5067847/ /pubmed/27774426 http://dx.doi.org/10.1016/j.jtcme.2015.09.002 Text en Copyright © 2016, Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Mahomoodally, M. Fawzi Dilmohamed, Schajeed Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
title | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
title_full | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
title_fullStr | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
title_full_unstemmed | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
title_short | Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
title_sort | antibacterial and antibiotic potentiating activity of vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067847/ https://www.ncbi.nlm.nih.gov/pubmed/27774426 http://dx.doi.org/10.1016/j.jtcme.2015.09.002 |
work_keys_str_mv | AT mahomoodallymfawzi antibacterialandantibioticpotentiatingactivityofvangueriamadagascariensisleavesandripefruitpericarpagainsthumanpathogenicclinicalbacterialisolates AT dilmohamedschajeed antibacterialandantibioticpotentiatingactivityofvangueriamadagascariensisleavesandripefruitpericarpagainsthumanpathogenicclinicalbacterialisolates |