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LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation

LIMKi 3 is a specific selective LIMK inhibitor against LIMK1 and LIMK2, while LIMK1 and LIMK2 are the main regulators of actin cytoskeleton to participate in many cell activities. However, the effect of LIMKi 3 in porcine oocyte meiosis is still unclear. The present study was designed to investigate...

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Autores principales: Jia, Ru-Xia, Duan, Xing, Song, Si-Jing, Sun, Shao-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068415/
https://www.ncbi.nlm.nih.gov/pubmed/27761340
http://dx.doi.org/10.7717/peerj.2553
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author Jia, Ru-Xia
Duan, Xing
Song, Si-Jing
Sun, Shao-Chen
author_facet Jia, Ru-Xia
Duan, Xing
Song, Si-Jing
Sun, Shao-Chen
author_sort Jia, Ru-Xia
collection PubMed
description LIMKi 3 is a specific selective LIMK inhibitor against LIMK1 and LIMK2, while LIMK1 and LIMK2 are the main regulators of actin cytoskeleton to participate in many cell activities. However, the effect of LIMKi 3 in porcine oocyte meiosis is still unclear. The present study was designed to investigate the effects of LIMKi 3 and potential regulatory role of LIMK1/2 on porcine oocyte meiotic maturation. Immunofluorescent staining of p-LIMK1/2 antibody showed that LIMK1/2 was localized mainly to the cortex of porcine oocyte, which co-localized with actin. After LIMKi 3 treatment, the diffusion of COCs became weak and the rate of polar body extrusion was decreased. This could be rescued by moving oocytes to fresh medium. After prolonging the culture time of oocytes, the maturation rate of porcine oocyte increased in LIMKi 3 groups, indicating that LIMKi 3 may suppress the cell cycle during porcine oocyte maturation. We also found that after LIMKi 3 treatment actin distribution was significantly disturbed at porcine oocyte membranes and cytoplasm, indicating the conserved roles of LIMK1/2 on actin dynamics. Next we examined the meiotic spindle positioning in porcine oocyte, and the results showed that a majority of spindles were not attached to the cortex of porcine oocyte, indicating that LIMKi 3 may affect actin-mediated spindle positioning. Taken together, these results showed that LIMK1/2 inhibitor LIMKi 3 had a repressive role on porcine oocyte meiotic maturation.
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spelling pubmed-50684152016-10-19 LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation Jia, Ru-Xia Duan, Xing Song, Si-Jing Sun, Shao-Chen PeerJ Cell Biology LIMKi 3 is a specific selective LIMK inhibitor against LIMK1 and LIMK2, while LIMK1 and LIMK2 are the main regulators of actin cytoskeleton to participate in many cell activities. However, the effect of LIMKi 3 in porcine oocyte meiosis is still unclear. The present study was designed to investigate the effects of LIMKi 3 and potential regulatory role of LIMK1/2 on porcine oocyte meiotic maturation. Immunofluorescent staining of p-LIMK1/2 antibody showed that LIMK1/2 was localized mainly to the cortex of porcine oocyte, which co-localized with actin. After LIMKi 3 treatment, the diffusion of COCs became weak and the rate of polar body extrusion was decreased. This could be rescued by moving oocytes to fresh medium. After prolonging the culture time of oocytes, the maturation rate of porcine oocyte increased in LIMKi 3 groups, indicating that LIMKi 3 may suppress the cell cycle during porcine oocyte maturation. We also found that after LIMKi 3 treatment actin distribution was significantly disturbed at porcine oocyte membranes and cytoplasm, indicating the conserved roles of LIMK1/2 on actin dynamics. Next we examined the meiotic spindle positioning in porcine oocyte, and the results showed that a majority of spindles were not attached to the cortex of porcine oocyte, indicating that LIMKi 3 may affect actin-mediated spindle positioning. Taken together, these results showed that LIMK1/2 inhibitor LIMKi 3 had a repressive role on porcine oocyte meiotic maturation. PeerJ Inc. 2016-10-12 /pmc/articles/PMC5068415/ /pubmed/27761340 http://dx.doi.org/10.7717/peerj.2553 Text en ©2016 Jia et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
Jia, Ru-Xia
Duan, Xing
Song, Si-Jing
Sun, Shao-Chen
LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation
title LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation
title_full LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation
title_fullStr LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation
title_full_unstemmed LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation
title_short LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation
title_sort limk1/2 inhibitor limki 3 suppresses porcine oocyte maturation
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068415/
https://www.ncbi.nlm.nih.gov/pubmed/27761340
http://dx.doi.org/10.7717/peerj.2553
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