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LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation
LIMKi 3 is a specific selective LIMK inhibitor against LIMK1 and LIMK2, while LIMK1 and LIMK2 are the main regulators of actin cytoskeleton to participate in many cell activities. However, the effect of LIMKi 3 in porcine oocyte meiosis is still unclear. The present study was designed to investigate...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068415/ https://www.ncbi.nlm.nih.gov/pubmed/27761340 http://dx.doi.org/10.7717/peerj.2553 |
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author | Jia, Ru-Xia Duan, Xing Song, Si-Jing Sun, Shao-Chen |
author_facet | Jia, Ru-Xia Duan, Xing Song, Si-Jing Sun, Shao-Chen |
author_sort | Jia, Ru-Xia |
collection | PubMed |
description | LIMKi 3 is a specific selective LIMK inhibitor against LIMK1 and LIMK2, while LIMK1 and LIMK2 are the main regulators of actin cytoskeleton to participate in many cell activities. However, the effect of LIMKi 3 in porcine oocyte meiosis is still unclear. The present study was designed to investigate the effects of LIMKi 3 and potential regulatory role of LIMK1/2 on porcine oocyte meiotic maturation. Immunofluorescent staining of p-LIMK1/2 antibody showed that LIMK1/2 was localized mainly to the cortex of porcine oocyte, which co-localized with actin. After LIMKi 3 treatment, the diffusion of COCs became weak and the rate of polar body extrusion was decreased. This could be rescued by moving oocytes to fresh medium. After prolonging the culture time of oocytes, the maturation rate of porcine oocyte increased in LIMKi 3 groups, indicating that LIMKi 3 may suppress the cell cycle during porcine oocyte maturation. We also found that after LIMKi 3 treatment actin distribution was significantly disturbed at porcine oocyte membranes and cytoplasm, indicating the conserved roles of LIMK1/2 on actin dynamics. Next we examined the meiotic spindle positioning in porcine oocyte, and the results showed that a majority of spindles were not attached to the cortex of porcine oocyte, indicating that LIMKi 3 may affect actin-mediated spindle positioning. Taken together, these results showed that LIMK1/2 inhibitor LIMKi 3 had a repressive role on porcine oocyte meiotic maturation. |
format | Online Article Text |
id | pubmed-5068415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50684152016-10-19 LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation Jia, Ru-Xia Duan, Xing Song, Si-Jing Sun, Shao-Chen PeerJ Cell Biology LIMKi 3 is a specific selective LIMK inhibitor against LIMK1 and LIMK2, while LIMK1 and LIMK2 are the main regulators of actin cytoskeleton to participate in many cell activities. However, the effect of LIMKi 3 in porcine oocyte meiosis is still unclear. The present study was designed to investigate the effects of LIMKi 3 and potential regulatory role of LIMK1/2 on porcine oocyte meiotic maturation. Immunofluorescent staining of p-LIMK1/2 antibody showed that LIMK1/2 was localized mainly to the cortex of porcine oocyte, which co-localized with actin. After LIMKi 3 treatment, the diffusion of COCs became weak and the rate of polar body extrusion was decreased. This could be rescued by moving oocytes to fresh medium. After prolonging the culture time of oocytes, the maturation rate of porcine oocyte increased in LIMKi 3 groups, indicating that LIMKi 3 may suppress the cell cycle during porcine oocyte maturation. We also found that after LIMKi 3 treatment actin distribution was significantly disturbed at porcine oocyte membranes and cytoplasm, indicating the conserved roles of LIMK1/2 on actin dynamics. Next we examined the meiotic spindle positioning in porcine oocyte, and the results showed that a majority of spindles were not attached to the cortex of porcine oocyte, indicating that LIMKi 3 may affect actin-mediated spindle positioning. Taken together, these results showed that LIMK1/2 inhibitor LIMKi 3 had a repressive role on porcine oocyte meiotic maturation. PeerJ Inc. 2016-10-12 /pmc/articles/PMC5068415/ /pubmed/27761340 http://dx.doi.org/10.7717/peerj.2553 Text en ©2016 Jia et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Cell Biology Jia, Ru-Xia Duan, Xing Song, Si-Jing Sun, Shao-Chen LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation |
title | LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation |
title_full | LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation |
title_fullStr | LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation |
title_full_unstemmed | LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation |
title_short | LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation |
title_sort | limk1/2 inhibitor limki 3 suppresses porcine oocyte maturation |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068415/ https://www.ncbi.nlm.nih.gov/pubmed/27761340 http://dx.doi.org/10.7717/peerj.2553 |
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