Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells

Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation...

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Autores principales: Di Liddo, Rosa, Aguiari, Paola, Barbon, Silvia, Bertalot, Thomas, Mandoli, Amit, Tasso, Alessia, Schrenk, Sandra, Iop, Laura, Gandaglia, Alessandro, Parnigotto, Pier Paolo, Conconi, Maria Teresa, Gerosa, Gino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068475/
https://www.ncbi.nlm.nih.gov/pubmed/27789941
http://dx.doi.org/10.2147/IJN.S115999
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author Di Liddo, Rosa
Aguiari, Paola
Barbon, Silvia
Bertalot, Thomas
Mandoli, Amit
Tasso, Alessia
Schrenk, Sandra
Iop, Laura
Gandaglia, Alessandro
Parnigotto, Pier Paolo
Conconi, Maria Teresa
Gerosa, Gino
author_facet Di Liddo, Rosa
Aguiari, Paola
Barbon, Silvia
Bertalot, Thomas
Mandoli, Amit
Tasso, Alessia
Schrenk, Sandra
Iop, Laura
Gandaglia, Alessandro
Parnigotto, Pier Paolo
Conconi, Maria Teresa
Gerosa, Gino
author_sort Di Liddo, Rosa
collection PubMed
description Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation potentiality. In order to define an in vitro model for investigating the influence of extracellular matrix signaling on the growth pattern of colonizing blood-derived cells, we cultured circulating multipotent cells (CMC) on acellular aortic (AVL) and pulmonary (PVL) valve conduits prepared with TriCol method and under no-flow condition. Isolated by our group from Vietnamese pigs before heart valve prosthetic implantation, porcine CMC revealed high proliferative abilities, three-lineage differentiative potential, and distinct hematopoietic/endothelial and mesenchymal properties. Their interaction with valve extracellular matrix nanostructures boosted differential messenger RNA expression pattern and morphologic features on AVL compared to PVL, while promoting on both matrices the commitment to valvular and endothelial cell-like phenotypes. Based on their origin from peripheral blood, porcine CMC are hypothesized in vivo to exert a pivotal role to homeostatically replenish valve cells and contribute to hetero- or allograft colonization. Furthermore, due to their high responsivity to extracellular matrix nanostructure signaling, porcine CMC could be useful for a preliminary evaluation of heart valve prosthetic functionality.
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spelling pubmed-50684752016-10-27 Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells Di Liddo, Rosa Aguiari, Paola Barbon, Silvia Bertalot, Thomas Mandoli, Amit Tasso, Alessia Schrenk, Sandra Iop, Laura Gandaglia, Alessandro Parnigotto, Pier Paolo Conconi, Maria Teresa Gerosa, Gino Int J Nanomedicine Original Research Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation potentiality. In order to define an in vitro model for investigating the influence of extracellular matrix signaling on the growth pattern of colonizing blood-derived cells, we cultured circulating multipotent cells (CMC) on acellular aortic (AVL) and pulmonary (PVL) valve conduits prepared with TriCol method and under no-flow condition. Isolated by our group from Vietnamese pigs before heart valve prosthetic implantation, porcine CMC revealed high proliferative abilities, three-lineage differentiative potential, and distinct hematopoietic/endothelial and mesenchymal properties. Their interaction with valve extracellular matrix nanostructures boosted differential messenger RNA expression pattern and morphologic features on AVL compared to PVL, while promoting on both matrices the commitment to valvular and endothelial cell-like phenotypes. Based on their origin from peripheral blood, porcine CMC are hypothesized in vivo to exert a pivotal role to homeostatically replenish valve cells and contribute to hetero- or allograft colonization. Furthermore, due to their high responsivity to extracellular matrix nanostructure signaling, porcine CMC could be useful for a preliminary evaluation of heart valve prosthetic functionality. Dove Medical Press 2016-10-12 /pmc/articles/PMC5068475/ /pubmed/27789941 http://dx.doi.org/10.2147/IJN.S115999 Text en © 2016 Di Liddo et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Di Liddo, Rosa
Aguiari, Paola
Barbon, Silvia
Bertalot, Thomas
Mandoli, Amit
Tasso, Alessia
Schrenk, Sandra
Iop, Laura
Gandaglia, Alessandro
Parnigotto, Pier Paolo
Conconi, Maria Teresa
Gerosa, Gino
Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_full Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_fullStr Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_full_unstemmed Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_short Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
title_sort nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068475/
https://www.ncbi.nlm.nih.gov/pubmed/27789941
http://dx.doi.org/10.2147/IJN.S115999
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