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Mitochondrial Genomes of Kinorhyncha: trnM Duplication and New Gene Orders within Animals

Many features of mitochondrial genomes of animals, such as patterns of gene arrangement, nucleotide content and substitution rate variation are extensively used in evolutionary and phylogenetic studies. Nearly 6,000 mitochondrial genomes of animals have already been sequenced, covering the majority...

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Autores principales: Popova, Olga V., Mikhailov, Kirill V., Nikitin, Mikhail A., Logacheva, Maria D., Penin, Aleksey A., Muntyan, Maria S., Kedrova, Olga S., Petrov, Nikolai B., Panchin, Yuri V., Aleoshin, Vladimir V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068742/
https://www.ncbi.nlm.nih.gov/pubmed/27755612
http://dx.doi.org/10.1371/journal.pone.0165072
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author Popova, Olga V.
Mikhailov, Kirill V.
Nikitin, Mikhail A.
Logacheva, Maria D.
Penin, Aleksey A.
Muntyan, Maria S.
Kedrova, Olga S.
Petrov, Nikolai B.
Panchin, Yuri V.
Aleoshin, Vladimir V.
author_facet Popova, Olga V.
Mikhailov, Kirill V.
Nikitin, Mikhail A.
Logacheva, Maria D.
Penin, Aleksey A.
Muntyan, Maria S.
Kedrova, Olga S.
Petrov, Nikolai B.
Panchin, Yuri V.
Aleoshin, Vladimir V.
author_sort Popova, Olga V.
collection PubMed
description Many features of mitochondrial genomes of animals, such as patterns of gene arrangement, nucleotide content and substitution rate variation are extensively used in evolutionary and phylogenetic studies. Nearly 6,000 mitochondrial genomes of animals have already been sequenced, covering the majority of animal phyla. One of the groups that escaped mitogenome sequencing is phylum Kinorhyncha—an isolated taxon of microscopic worm-like ecdysozoans. The kinorhynchs are thought to be one of the early-branching lineages of Ecdysozoa, and their mitochondrial genomes may be important for resolving evolutionary relations between major animal taxa. Here we present the results of sequencing and analysis of mitochondrial genomes from two members of Kinorhyncha, Echinoderes svetlanae (Cyclorhagida) and Pycnophyes kielensis (Allomalorhagida). Their mitochondrial genomes are circular molecules approximately 15 Kbp in size. The kinorhynch mitochondrial gene sequences are highly divergent, which precludes accurate phylogenetic inference. The mitogenomes of both species encode a typical metazoan complement of 37 genes, which are all positioned on the major strand, but the gene order is distinct and unique among Ecdysozoa or animals as a whole. We predict four types of start codons for protein-coding genes in E. svetlanae and five in P. kielensis with a consensus DTD in single letter code. The mitochondrial genomes of E. svetlanae and P. kielensis encode duplicated methionine tRNA genes that display compensatory nucleotide substitutions. Two distant species of Kinorhyncha demonstrate similar patterns of gene arrangements in their mitogenomes. Both genomes have duplicated methionine tRNA genes; the duplication predates the divergence of two species. The kinorhynchs share a few features pertaining to gene order that align them with Priapulida. Gene order analysis reveals that gene arrangement specific of Priapulida may be ancestral for Scalidophora, Ecdysozoa, and even Protostomia.
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spelling pubmed-50687422016-10-27 Mitochondrial Genomes of Kinorhyncha: trnM Duplication and New Gene Orders within Animals Popova, Olga V. Mikhailov, Kirill V. Nikitin, Mikhail A. Logacheva, Maria D. Penin, Aleksey A. Muntyan, Maria S. Kedrova, Olga S. Petrov, Nikolai B. Panchin, Yuri V. Aleoshin, Vladimir V. PLoS One Research Article Many features of mitochondrial genomes of animals, such as patterns of gene arrangement, nucleotide content and substitution rate variation are extensively used in evolutionary and phylogenetic studies. Nearly 6,000 mitochondrial genomes of animals have already been sequenced, covering the majority of animal phyla. One of the groups that escaped mitogenome sequencing is phylum Kinorhyncha—an isolated taxon of microscopic worm-like ecdysozoans. The kinorhynchs are thought to be one of the early-branching lineages of Ecdysozoa, and their mitochondrial genomes may be important for resolving evolutionary relations between major animal taxa. Here we present the results of sequencing and analysis of mitochondrial genomes from two members of Kinorhyncha, Echinoderes svetlanae (Cyclorhagida) and Pycnophyes kielensis (Allomalorhagida). Their mitochondrial genomes are circular molecules approximately 15 Kbp in size. The kinorhynch mitochondrial gene sequences are highly divergent, which precludes accurate phylogenetic inference. The mitogenomes of both species encode a typical metazoan complement of 37 genes, which are all positioned on the major strand, but the gene order is distinct and unique among Ecdysozoa or animals as a whole. We predict four types of start codons for protein-coding genes in E. svetlanae and five in P. kielensis with a consensus DTD in single letter code. The mitochondrial genomes of E. svetlanae and P. kielensis encode duplicated methionine tRNA genes that display compensatory nucleotide substitutions. Two distant species of Kinorhyncha demonstrate similar patterns of gene arrangements in their mitogenomes. Both genomes have duplicated methionine tRNA genes; the duplication predates the divergence of two species. The kinorhynchs share a few features pertaining to gene order that align them with Priapulida. Gene order analysis reveals that gene arrangement specific of Priapulida may be ancestral for Scalidophora, Ecdysozoa, and even Protostomia. Public Library of Science 2016-10-18 /pmc/articles/PMC5068742/ /pubmed/27755612 http://dx.doi.org/10.1371/journal.pone.0165072 Text en © 2016 Popova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Popova, Olga V.
Mikhailov, Kirill V.
Nikitin, Mikhail A.
Logacheva, Maria D.
Penin, Aleksey A.
Muntyan, Maria S.
Kedrova, Olga S.
Petrov, Nikolai B.
Panchin, Yuri V.
Aleoshin, Vladimir V.
Mitochondrial Genomes of Kinorhyncha: trnM Duplication and New Gene Orders within Animals
title Mitochondrial Genomes of Kinorhyncha: trnM Duplication and New Gene Orders within Animals
title_full Mitochondrial Genomes of Kinorhyncha: trnM Duplication and New Gene Orders within Animals
title_fullStr Mitochondrial Genomes of Kinorhyncha: trnM Duplication and New Gene Orders within Animals
title_full_unstemmed Mitochondrial Genomes of Kinorhyncha: trnM Duplication and New Gene Orders within Animals
title_short Mitochondrial Genomes of Kinorhyncha: trnM Duplication and New Gene Orders within Animals
title_sort mitochondrial genomes of kinorhyncha: trnm duplication and new gene orders within animals
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068742/
https://www.ncbi.nlm.nih.gov/pubmed/27755612
http://dx.doi.org/10.1371/journal.pone.0165072
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