Chromosome Specific Substitution Lines of Aegilops geniculata Alter Parameters of Bread Making Quality of Wheat

Wheat cultivars with wide introgression have strongly impacted global wheat production. Aegilops geniculata (M(g)U(g)) is an important wild relative with several useful traits that can be exploited for wheat improvement. Screening of Ae. geniculata addition lines indicated a negative effect of 1U(g) and the positive effect of 1M(g) chromosome on wheat dough strength. Negative effect of 1U(g) is probably associated with variation in number and position of the tripeptide repeat motif in the high molecular weight glutenin (HMW-G) gene. To utilize the positive potential of 1M(g) chromosome, three disomic substitution lines (DSLs) 1M(g)(1A), 1M(g)(1B) and 1M(g)(1D) were created. These lines were characterized for morphological, cytogenetic properties and biochemical signatures using FISH, 1D-, 2D-PAGE and RP-HPLC. Contribution of wheat 1A, 1B and 1D chromosomes towards dough mixing and baking parameters, chapatti quality, Fe/Zn content and glume color were identified. Observed order of variation in the dough mixing and baking parameters {1M(g)(1D) ≤wheat ≤1M(g)(1B) ≤1M(g)(1A)} indicated that chromosome specific introgression is desirable for best utilization of wild species’ potential.