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Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells

BACKGROUND: Enamel matrix derivative (EMD) is an effective biomaterial for periodontal tissue regeneration and might stimulate angiogenesis. Tyrosine-rich amelogenin peptide (TRAP) is present in EMD and is thought to contribute in its biological activity. In the present study, we investigated the ef...

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Autores principales: Jonke, Erwin, Gemperli, Anja C., Zhang, Taowen, Özdemir, Burcu, Dard, Michel, Rausch-Fan, Xiaohui, Andrukhov, Oleh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069334/
https://www.ncbi.nlm.nih.gov/pubmed/26867593
http://dx.doi.org/10.1007/s00784-016-1726-2
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author Jonke, Erwin
Gemperli, Anja C.
Zhang, Taowen
Özdemir, Burcu
Dard, Michel
Rausch-Fan, Xiaohui
Andrukhov, Oleh
author_facet Jonke, Erwin
Gemperli, Anja C.
Zhang, Taowen
Özdemir, Burcu
Dard, Michel
Rausch-Fan, Xiaohui
Andrukhov, Oleh
author_sort Jonke, Erwin
collection PubMed
description BACKGROUND: Enamel matrix derivative (EMD) is an effective biomaterial for periodontal tissue regeneration and might stimulate angiogenesis. Tyrosine-rich amelogenin peptide (TRAP) is present in EMD and is thought to contribute in its biological activity. In the present study, we investigated the effect of chemically synthesized TRAP on proliferation, migration, angiogenic structure formation, and differentiation of human umbilical vein endothelial cells (HUVECs) in vitro. MATERIAL AND METHODS: The effects of TRAP isolated from EMD and chemically synthesized TRAP on proliferation/viability, migration, and angiogenic structure formation were investigated. Expression of angiopoietin-2 (ang-2), von Willebrand factor (vWF), E-selectin, intracellular adhesion molecules 1 (ICAM-1), vascular endothelial growth factor (VEGF) receptors FMS-like tyrosine kinase 1 (FLT-1), and kinase insert domain receptor (KDR) was measured on both messenger RNA (mRNA) and protein levels. RESULTS: The proliferation/viability of HUVECs was inhibited by TRAP at concentration of 100 μg/ml and slightly stimulated by EMD at similar concentration. Both EMD and TRAP stimulated endothelial cell migration in microchemotaxis chamber. The effect of both TRAP preparations on the migration was significantly higher than that of EMD. All substances stimulated formation of angiogenic structure in vitro. The expression of ICAM-1, E-selectin, FLT-1, KDR, and vWF was significantly increased by both TRAP and EMD at a concentration 50 μg/ml. The expression of ang-2 was not affected by TRAP but was significantly increased by EMD. CONCLUSION: Our in vitro study shows that TRAP confer the most effects of EMD on the endothelial cells. CLINICAL RELEVANCE: TRAP might be used as a basis for development of new approaches for periodontal regeneration.
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spelling pubmed-50693342016-11-02 Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells Jonke, Erwin Gemperli, Anja C. Zhang, Taowen Özdemir, Burcu Dard, Michel Rausch-Fan, Xiaohui Andrukhov, Oleh Clin Oral Investig Original Article BACKGROUND: Enamel matrix derivative (EMD) is an effective biomaterial for periodontal tissue regeneration and might stimulate angiogenesis. Tyrosine-rich amelogenin peptide (TRAP) is present in EMD and is thought to contribute in its biological activity. In the present study, we investigated the effect of chemically synthesized TRAP on proliferation, migration, angiogenic structure formation, and differentiation of human umbilical vein endothelial cells (HUVECs) in vitro. MATERIAL AND METHODS: The effects of TRAP isolated from EMD and chemically synthesized TRAP on proliferation/viability, migration, and angiogenic structure formation were investigated. Expression of angiopoietin-2 (ang-2), von Willebrand factor (vWF), E-selectin, intracellular adhesion molecules 1 (ICAM-1), vascular endothelial growth factor (VEGF) receptors FMS-like tyrosine kinase 1 (FLT-1), and kinase insert domain receptor (KDR) was measured on both messenger RNA (mRNA) and protein levels. RESULTS: The proliferation/viability of HUVECs was inhibited by TRAP at concentration of 100 μg/ml and slightly stimulated by EMD at similar concentration. Both EMD and TRAP stimulated endothelial cell migration in microchemotaxis chamber. The effect of both TRAP preparations on the migration was significantly higher than that of EMD. All substances stimulated formation of angiogenic structure in vitro. The expression of ICAM-1, E-selectin, FLT-1, KDR, and vWF was significantly increased by both TRAP and EMD at a concentration 50 μg/ml. The expression of ang-2 was not affected by TRAP but was significantly increased by EMD. CONCLUSION: Our in vitro study shows that TRAP confer the most effects of EMD on the endothelial cells. CLINICAL RELEVANCE: TRAP might be used as a basis for development of new approaches for periodontal regeneration. Springer Berlin Heidelberg 2016-02-12 2016 /pmc/articles/PMC5069334/ /pubmed/26867593 http://dx.doi.org/10.1007/s00784-016-1726-2 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Jonke, Erwin
Gemperli, Anja C.
Zhang, Taowen
Özdemir, Burcu
Dard, Michel
Rausch-Fan, Xiaohui
Andrukhov, Oleh
Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells
title Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells
title_full Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells
title_fullStr Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells
title_full_unstemmed Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells
title_short Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells
title_sort effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069334/
https://www.ncbi.nlm.nih.gov/pubmed/26867593
http://dx.doi.org/10.1007/s00784-016-1726-2
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