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Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish

The nephron is the functional unit of the kidney and is divided into distinct proximal and distal segments. The factors determining nephron segment size are not fully understood. In zebrafish, the embryonic kidney has long been thought to differentiate in situ into two proximal tubule segments and t...

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Autores principales: Naylor, Richard W., Dodd, Rachel C., Davidson, Alan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069491/
https://www.ncbi.nlm.nih.gov/pubmed/27759103
http://dx.doi.org/10.1038/srep35647
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author Naylor, Richard W.
Dodd, Rachel C.
Davidson, Alan J.
author_facet Naylor, Richard W.
Dodd, Rachel C.
Davidson, Alan J.
author_sort Naylor, Richard W.
collection PubMed
description The nephron is the functional unit of the kidney and is divided into distinct proximal and distal segments. The factors determining nephron segment size are not fully understood. In zebrafish, the embryonic kidney has long been thought to differentiate in situ into two proximal tubule segments and two distal tubule segments (distal early; DE, and distal late; DL) with little involvement of cell movement. Here, we overturn this notion by performing lineage-labelling experiments that reveal extensive caudal movement of the proximal and DE segments and a concomitant compaction of the DL segment as it fuses with the cloaca. Laser-mediated severing of the tubule, such that the DE and DL are disconnected or that the DL and cloaca do not fuse, results in a reduction in tubule cell proliferation and significantly shortens the DE segment while the caudal movement of the DL is unaffected. These results suggest that the DL mechanically pulls the more proximal segments, thereby driving both their caudal extension and their proliferation. Together, these data provide new insights into early nephron morphogenesis and demonstrate the importance of cell movement and proliferation in determining initial nephron segment size.
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spelling pubmed-50694912016-10-26 Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish Naylor, Richard W. Dodd, Rachel C. Davidson, Alan J. Sci Rep Article The nephron is the functional unit of the kidney and is divided into distinct proximal and distal segments. The factors determining nephron segment size are not fully understood. In zebrafish, the embryonic kidney has long been thought to differentiate in situ into two proximal tubule segments and two distal tubule segments (distal early; DE, and distal late; DL) with little involvement of cell movement. Here, we overturn this notion by performing lineage-labelling experiments that reveal extensive caudal movement of the proximal and DE segments and a concomitant compaction of the DL segment as it fuses with the cloaca. Laser-mediated severing of the tubule, such that the DE and DL are disconnected or that the DL and cloaca do not fuse, results in a reduction in tubule cell proliferation and significantly shortens the DE segment while the caudal movement of the DL is unaffected. These results suggest that the DL mechanically pulls the more proximal segments, thereby driving both their caudal extension and their proliferation. Together, these data provide new insights into early nephron morphogenesis and demonstrate the importance of cell movement and proliferation in determining initial nephron segment size. Nature Publishing Group 2016-10-19 /pmc/articles/PMC5069491/ /pubmed/27759103 http://dx.doi.org/10.1038/srep35647 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Naylor, Richard W.
Dodd, Rachel C.
Davidson, Alan J.
Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish
title Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish
title_full Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish
title_fullStr Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish
title_full_unstemmed Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish
title_short Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish
title_sort caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069491/
https://www.ncbi.nlm.nih.gov/pubmed/27759103
http://dx.doi.org/10.1038/srep35647
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