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Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish
The nephron is the functional unit of the kidney and is divided into distinct proximal and distal segments. The factors determining nephron segment size are not fully understood. In zebrafish, the embryonic kidney has long been thought to differentiate in situ into two proximal tubule segments and t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069491/ https://www.ncbi.nlm.nih.gov/pubmed/27759103 http://dx.doi.org/10.1038/srep35647 |
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author | Naylor, Richard W. Dodd, Rachel C. Davidson, Alan J. |
author_facet | Naylor, Richard W. Dodd, Rachel C. Davidson, Alan J. |
author_sort | Naylor, Richard W. |
collection | PubMed |
description | The nephron is the functional unit of the kidney and is divided into distinct proximal and distal segments. The factors determining nephron segment size are not fully understood. In zebrafish, the embryonic kidney has long been thought to differentiate in situ into two proximal tubule segments and two distal tubule segments (distal early; DE, and distal late; DL) with little involvement of cell movement. Here, we overturn this notion by performing lineage-labelling experiments that reveal extensive caudal movement of the proximal and DE segments and a concomitant compaction of the DL segment as it fuses with the cloaca. Laser-mediated severing of the tubule, such that the DE and DL are disconnected or that the DL and cloaca do not fuse, results in a reduction in tubule cell proliferation and significantly shortens the DE segment while the caudal movement of the DL is unaffected. These results suggest that the DL mechanically pulls the more proximal segments, thereby driving both their caudal extension and their proliferation. Together, these data provide new insights into early nephron morphogenesis and demonstrate the importance of cell movement and proliferation in determining initial nephron segment size. |
format | Online Article Text |
id | pubmed-5069491 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50694912016-10-26 Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish Naylor, Richard W. Dodd, Rachel C. Davidson, Alan J. Sci Rep Article The nephron is the functional unit of the kidney and is divided into distinct proximal and distal segments. The factors determining nephron segment size are not fully understood. In zebrafish, the embryonic kidney has long been thought to differentiate in situ into two proximal tubule segments and two distal tubule segments (distal early; DE, and distal late; DL) with little involvement of cell movement. Here, we overturn this notion by performing lineage-labelling experiments that reveal extensive caudal movement of the proximal and DE segments and a concomitant compaction of the DL segment as it fuses with the cloaca. Laser-mediated severing of the tubule, such that the DE and DL are disconnected or that the DL and cloaca do not fuse, results in a reduction in tubule cell proliferation and significantly shortens the DE segment while the caudal movement of the DL is unaffected. These results suggest that the DL mechanically pulls the more proximal segments, thereby driving both their caudal extension and their proliferation. Together, these data provide new insights into early nephron morphogenesis and demonstrate the importance of cell movement and proliferation in determining initial nephron segment size. Nature Publishing Group 2016-10-19 /pmc/articles/PMC5069491/ /pubmed/27759103 http://dx.doi.org/10.1038/srep35647 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Naylor, Richard W. Dodd, Rachel C. Davidson, Alan J. Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish |
title | Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish |
title_full | Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish |
title_fullStr | Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish |
title_full_unstemmed | Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish |
title_short | Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish |
title_sort | caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069491/ https://www.ncbi.nlm.nih.gov/pubmed/27759103 http://dx.doi.org/10.1038/srep35647 |
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