Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology

HPV integration plays an important role in cervical carcinogenesis. HPV genotypes and the exact integration sites were investigated using HPV capture technology combined with next generation sequencing in 166 women. Three, one and six integration sites were verified in 7 HPV-positive ‘normal cervica...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Ying, Zhang, Chaoting, Gao, Weijiao, Wang, Limin, Pan, Yaqi, Gao, Yunong, Lu, Zheming, Ke, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069689/
https://www.ncbi.nlm.nih.gov/pubmed/27759101
http://dx.doi.org/10.1038/srep35427
_version_ 1782460987676295168
author Liu, Ying
Zhang, Chaoting
Gao, Weijiao
Wang, Limin
Pan, Yaqi
Gao, Yunong
Lu, Zheming
Ke, Yang
author_facet Liu, Ying
Zhang, Chaoting
Gao, Weijiao
Wang, Limin
Pan, Yaqi
Gao, Yunong
Lu, Zheming
Ke, Yang
author_sort Liu, Ying
collection PubMed
description HPV integration plays an important role in cervical carcinogenesis. HPV genotypes and the exact integration sites were investigated using HPV capture technology combined with next generation sequencing in 166 women. Three, one and six integration sites were verified in 7 HPV-positive ‘normal cervical epithelium’, 6 HPV-positive CIN2 and 15 HPV-positive CIN 3 samples, respectively. Of the 10 integrations, one and nine were involved with HPV33 and HPV16, respectively. Our study accurately evaluated HPV integration level in CINs and normal cervical tissues using high-throughput viral integration detection method providing basic evidence for HPV integration-driven cervical carcinogenesis.
format Online
Article
Text
id pubmed-5069689
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-50696892016-10-26 Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology Liu, Ying Zhang, Chaoting Gao, Weijiao Wang, Limin Pan, Yaqi Gao, Yunong Lu, Zheming Ke, Yang Sci Rep Article HPV integration plays an important role in cervical carcinogenesis. HPV genotypes and the exact integration sites were investigated using HPV capture technology combined with next generation sequencing in 166 women. Three, one and six integration sites were verified in 7 HPV-positive ‘normal cervical epithelium’, 6 HPV-positive CIN2 and 15 HPV-positive CIN 3 samples, respectively. Of the 10 integrations, one and nine were involved with HPV33 and HPV16, respectively. Our study accurately evaluated HPV integration level in CINs and normal cervical tissues using high-throughput viral integration detection method providing basic evidence for HPV integration-driven cervical carcinogenesis. Nature Publishing Group 2016-10-19 /pmc/articles/PMC5069689/ /pubmed/27759101 http://dx.doi.org/10.1038/srep35427 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Ying
Zhang, Chaoting
Gao, Weijiao
Wang, Limin
Pan, Yaqi
Gao, Yunong
Lu, Zheming
Ke, Yang
Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology
title Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology
title_full Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology
title_fullStr Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology
title_full_unstemmed Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology
title_short Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology
title_sort genome-wide profiling of the human papillomavirus dna integration in cervical intraepithelial neoplasia and normal cervical epithelium by hpv capture technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069689/
https://www.ncbi.nlm.nih.gov/pubmed/27759101
http://dx.doi.org/10.1038/srep35427
work_keys_str_mv AT liuying genomewideprofilingofthehumanpapillomavirusdnaintegrationincervicalintraepithelialneoplasiaandnormalcervicalepitheliumbyhpvcapturetechnology
AT zhangchaoting genomewideprofilingofthehumanpapillomavirusdnaintegrationincervicalintraepithelialneoplasiaandnormalcervicalepitheliumbyhpvcapturetechnology
AT gaoweijiao genomewideprofilingofthehumanpapillomavirusdnaintegrationincervicalintraepithelialneoplasiaandnormalcervicalepitheliumbyhpvcapturetechnology
AT wanglimin genomewideprofilingofthehumanpapillomavirusdnaintegrationincervicalintraepithelialneoplasiaandnormalcervicalepitheliumbyhpvcapturetechnology
AT panyaqi genomewideprofilingofthehumanpapillomavirusdnaintegrationincervicalintraepithelialneoplasiaandnormalcervicalepitheliumbyhpvcapturetechnology
AT gaoyunong genomewideprofilingofthehumanpapillomavirusdnaintegrationincervicalintraepithelialneoplasiaandnormalcervicalepitheliumbyhpvcapturetechnology
AT luzheming genomewideprofilingofthehumanpapillomavirusdnaintegrationincervicalintraepithelialneoplasiaandnormalcervicalepitheliumbyhpvcapturetechnology
AT keyang genomewideprofilingofthehumanpapillomavirusdnaintegrationincervicalintraepithelialneoplasiaandnormalcervicalepitheliumbyhpvcapturetechnology

Ejemplares similares