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Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility

This in-vitro study introduces residual stress as a third dimension of cell stimulus to modulate the interaction between cells and bio-template, without the addition of either chemical or physical stimuli onto the bio-template surface. Ultrashort Pulsed Laser (USPL) irradiation of silicon-based bio-...

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Autores principales: Keshavarz, Meysam, Tan, Bo, Venkatakrishnan, Krishnan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069693/
https://www.ncbi.nlm.nih.gov/pubmed/27759054
http://dx.doi.org/10.1038/srep35425
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author Keshavarz, Meysam
Tan, Bo
Venkatakrishnan, Krishnan
author_facet Keshavarz, Meysam
Tan, Bo
Venkatakrishnan, Krishnan
author_sort Keshavarz, Meysam
collection PubMed
description This in-vitro study introduces residual stress as a third dimension of cell stimulus to modulate the interaction between cells and bio-template, without the addition of either chemical or physical stimuli onto the bio-template surface. Ultrashort Pulsed Laser (USPL) irradiation of silicon-based bio-template causes recrystallization of silicon, which mismatches the original crystal orientation of the virgin silicon. Consequently, subsurface Induced Residual Stress (IRS) is generated. The IRS components demonstrated a strong cytocompatibility, whereas the peripheral of IRS, which is the interface between the IRS component and the virgin silicon surface, a significant directional cell alignment was observed. Fibroblast cells shown to be more sensitive to the stress component than Hela cancer cells. It revealed that cytocompatibility in terms of cell migration and directional cell alignment is directly proportional to the level of the IRS component. Higher stress level results in more cell alignment and border migration width. There is a stress threshold below which the stress component completely loses the functionality. These results pointed to a functionalized bio-template with tunable cytocompatibility. This study may lead to a new tool for the designing and engineering of bio-template.
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spelling pubmed-50696932016-10-26 Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility Keshavarz, Meysam Tan, Bo Venkatakrishnan, Krishnan Sci Rep Article This in-vitro study introduces residual stress as a third dimension of cell stimulus to modulate the interaction between cells and bio-template, without the addition of either chemical or physical stimuli onto the bio-template surface. Ultrashort Pulsed Laser (USPL) irradiation of silicon-based bio-template causes recrystallization of silicon, which mismatches the original crystal orientation of the virgin silicon. Consequently, subsurface Induced Residual Stress (IRS) is generated. The IRS components demonstrated a strong cytocompatibility, whereas the peripheral of IRS, which is the interface between the IRS component and the virgin silicon surface, a significant directional cell alignment was observed. Fibroblast cells shown to be more sensitive to the stress component than Hela cancer cells. It revealed that cytocompatibility in terms of cell migration and directional cell alignment is directly proportional to the level of the IRS component. Higher stress level results in more cell alignment and border migration width. There is a stress threshold below which the stress component completely loses the functionality. These results pointed to a functionalized bio-template with tunable cytocompatibility. This study may lead to a new tool for the designing and engineering of bio-template. Nature Publishing Group 2016-10-19 /pmc/articles/PMC5069693/ /pubmed/27759054 http://dx.doi.org/10.1038/srep35425 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Keshavarz, Meysam
Tan, Bo
Venkatakrishnan, Krishnan
Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility
title Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility
title_full Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility
title_fullStr Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility
title_full_unstemmed Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility
title_short Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility
title_sort functionalized stress component onto bio-template as a pathway of cytocompatibility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069693/
https://www.ncbi.nlm.nih.gov/pubmed/27759054
http://dx.doi.org/10.1038/srep35425
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