A Universal Next-Generation Sequencing Protocol To Generate Noninfectious Barcoded cDNA Libraries from High-Containment RNA Viruses

Several biosafety level 3 and/or 4 (BSL-3/4) pathogens are high-consequence, single-stranded RNA viruses, and their genomes, when introduced into permissive cells, are infectious. Moreover, many of these viruses are select agents (SAs), and their genomes are also considered SAs. For this reason, cDN...

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Detalles Bibliográficos
Autores principales: Moser, Lindsey A., Ramirez-Carvajal, Lisbeth, Puri, Vinita, Pauszek, Steven J., Matthews, Krystal, Dilley, Kari A., Mullan, Clancy, McGraw, Jennifer, Khayat, Michael, Beeri, Karen, Yee, Anthony, Dugan, Vivien, Heise, Mark T., Frieman, Matthew B., Rodriguez, Luis L., Bernard, Kristen A., Wentworth, David E., Stockwell, Timothy B., Shabman, Reed S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069770/
https://www.ncbi.nlm.nih.gov/pubmed/27822536
http://dx.doi.org/10.1128/mSystems.00039-15

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