A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg(2+)–T mismatched DNA

We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg(2+). Unlike common DNA-based Hg(2+) detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Wanhe, Kang, Tian-Shu, Chan, Philip Wai Hong, Lu, Jin-Jian, Chen, Xiu-Ping, Leung, Chung-Hang, Ma, Dik-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069990/
https://www.ncbi.nlm.nih.gov/pubmed/27877846
http://dx.doi.org/10.1088/1468-6996/16/6/065004
_version_ 1782461049551716352
author Wang, Wanhe
Kang, Tian-Shu
Chan, Philip Wai Hong
Lu, Jin-Jian
Chen, Xiu-Ping
Leung, Chung-Hang
Ma, Dik-Lung
author_facet Wang, Wanhe
Kang, Tian-Shu
Chan, Philip Wai Hong
Lu, Jin-Jian
Chen, Xiu-Ping
Leung, Chung-Hang
Ma, Dik-Lung
author_sort Wang, Wanhe
collection PubMed
description We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg(2+). Unlike common DNA-based Hg(2+) detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This ‘mix-and-detect’ methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg(2+).
format Online
Article
Text
id pubmed-5069990
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-50699902016-11-22 A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg(2+)–T mismatched DNA Wang, Wanhe Kang, Tian-Shu Chan, Philip Wai Hong Lu, Jin-Jian Chen, Xiu-Ping Leung, Chung-Hang Ma, Dik-Lung Sci Technol Adv Mater Papers We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg(2+). Unlike common DNA-based Hg(2+) detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This ‘mix-and-detect’ methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg(2+). Taylor & Francis 2015-11-17 /pmc/articles/PMC5069990/ /pubmed/27877846 http://dx.doi.org/10.1088/1468-6996/16/6/065004 Text en © 2015 National Institute for Materials Science http://creativecommons.org/licenses/by/3.0/ Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence (http://creativecommons.org/licenses/by/3.0) . Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
spellingShingle Papers
Wang, Wanhe
Kang, Tian-Shu
Chan, Philip Wai Hong
Lu, Jin-Jian
Chen, Xiu-Ping
Leung, Chung-Hang
Ma, Dik-Lung
A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg(2+)–T mismatched DNA
title A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg(2+)–T mismatched DNA
title_full A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg(2+)–T mismatched DNA
title_fullStr A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg(2+)–T mismatched DNA
title_full_unstemmed A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg(2+)–T mismatched DNA
title_short A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg(2+)–T mismatched DNA
title_sort label-free g-quadruplex-based mercury detection assay employing the exonuclease iii-mediated cleavage of t–hg(2+)–t mismatched dna
topic Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069990/
https://www.ncbi.nlm.nih.gov/pubmed/27877846
http://dx.doi.org/10.1088/1468-6996/16/6/065004
work_keys_str_mv AT wangwanhe alabelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT kangtianshu alabelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT chanphilipwaihong alabelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT lujinjian alabelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT chenxiuping alabelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT leungchunghang alabelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT madiklung alabelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT wangwanhe labelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT kangtianshu labelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT chanphilipwaihong labelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT lujinjian labelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT chenxiuping labelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT leungchunghang labelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna
AT madiklung labelfreegquadruplexbasedmercurydetectionassayemployingtheexonucleaseiiimediatedcleavageofthg2tmismatcheddna

Ejemplares similares