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Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells

BACKGROUND: Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter devoid of CpG dinucleotides, CpGfree, in parallel with a similar CpG containing...

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Detalles Bibliográficos
Autores principales:	Ho, Steven C. L., Koh, Esther Y. C., Soo, Benjamin P. C., Mariati, Chao, Sheng-Hao, Yang, Yuansheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5070371/ https://www.ncbi.nlm.nih.gov/pubmed/27756290 http://dx.doi.org/10.1186/s12896-016-0300-y

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