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Identification of amino acids related to catalytic function of Sulfolobus solfataricus P1 carboxylesterase by site-directed mutagenesis and molecular modeling

The archaeon Sulfolobus solfataricus P1 carboxylesterase is a thermostable enzyme with a molecular mass of 33.5 kDa belonging to the mammalian hormone-sensitive lipase (HSL) family. In our previous study, we purified the enzyme and suggested the expected amino acids related to its catalysis by chemi...

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Detalles Bibliográficos
Autores principales:	Choi, Yun-Ho, Lee, Ye-Na, Park, Young-Jun, Yoon, Sung-Jin, Lee, Hee-Bong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Korean Society for Biochemistry and Molecular Biology 2016
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5070724/ https://www.ncbi.nlm.nih.gov/pubmed/27222124 http://dx.doi.org/10.5483/BMBRep.2016.49.6.077

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