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TRPM7 is down‐regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function

AIMS: The kinase ion channel transient receptor potential melastatin 7 (TRPM7) is considered a modulator of cardiac fibrosis progression; nevertheless, we lack of studies analysing its role in human ischaemic cardiomyopathy (ICM). Our objective was to analyse the expression of genes encoding cardiac...

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Autores principales: Ortega, Ana, Roselló‐Lletí, Esther, Tarazón, Estefanía, Gil‐Cayuela, Carolina, Lago, Francisca, González‐Juanatey, Jose‐Ramón, Martinez‐Dolz, Luis, Portolés, Manuel, Rivera, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5071679/
https://www.ncbi.nlm.nih.gov/pubmed/27818786
http://dx.doi.org/10.1002/ehf2.12085
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author Ortega, Ana
Roselló‐Lletí, Esther
Tarazón, Estefanía
Gil‐Cayuela, Carolina
Lago, Francisca
González‐Juanatey, Jose‐Ramón
Martinez‐Dolz, Luis
Portolés, Manuel
Rivera, Miguel
author_facet Ortega, Ana
Roselló‐Lletí, Esther
Tarazón, Estefanía
Gil‐Cayuela, Carolina
Lago, Francisca
González‐Juanatey, Jose‐Ramón
Martinez‐Dolz, Luis
Portolés, Manuel
Rivera, Miguel
author_sort Ortega, Ana
collection PubMed
description AIMS: The kinase ion channel transient receptor potential melastatin 7 (TRPM7) is considered a modulator of cardiac fibrosis progression; nevertheless, we lack of studies analysing its role in human ischaemic cardiomyopathy (ICM). Our objective was to analyse the expression of genes encoding cardiac ion channels in human ICM, focusing on the alterations in mRNA levels of TRPM7 and its relationship with changes in the ventricular function. METHODS AND RESULTS: RNA‐sequencing was carried out in 13 left ventricular (LV) samples of patients with ICM compared with a control group (n = 10). The analysis revealed a total of 25 ion channel genes differentially expressed. We performed an RTqPCR analysis of the TRPM7 mRNA in LV and left atrial samples and found that it was down‐regulated in both cavities (−1.43‐fold and −1.52‐fold, respectively). Atrial TRPM7 mRNA levels showed an excellent and inverse relationships with the depressed ejection fraction (r = −0.724, P = 0.042) and with the mitral A wave (r = −0.938, P = 0.006). CONCLUSIONS: We report the down‐regulation of TRPM7 in tissue samples from both left atria and left ventricle in patients with ICM. We found an inverse relationship between both cardiac chambers mRNA levels with LV dysfunction, suggesting an important role of TRPM7 in the left atrial and LV functional depression found in this cardiomyopathy.
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spelling pubmed-50716792016-11-02 TRPM7 is down‐regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function Ortega, Ana Roselló‐Lletí, Esther Tarazón, Estefanía Gil‐Cayuela, Carolina Lago, Francisca González‐Juanatey, Jose‐Ramón Martinez‐Dolz, Luis Portolés, Manuel Rivera, Miguel ESC Heart Fail Short Communication AIMS: The kinase ion channel transient receptor potential melastatin 7 (TRPM7) is considered a modulator of cardiac fibrosis progression; nevertheless, we lack of studies analysing its role in human ischaemic cardiomyopathy (ICM). Our objective was to analyse the expression of genes encoding cardiac ion channels in human ICM, focusing on the alterations in mRNA levels of TRPM7 and its relationship with changes in the ventricular function. METHODS AND RESULTS: RNA‐sequencing was carried out in 13 left ventricular (LV) samples of patients with ICM compared with a control group (n = 10). The analysis revealed a total of 25 ion channel genes differentially expressed. We performed an RTqPCR analysis of the TRPM7 mRNA in LV and left atrial samples and found that it was down‐regulated in both cavities (−1.43‐fold and −1.52‐fold, respectively). Atrial TRPM7 mRNA levels showed an excellent and inverse relationships with the depressed ejection fraction (r = −0.724, P = 0.042) and with the mitral A wave (r = −0.938, P = 0.006). CONCLUSIONS: We report the down‐regulation of TRPM7 in tissue samples from both left atria and left ventricle in patients with ICM. We found an inverse relationship between both cardiac chambers mRNA levels with LV dysfunction, suggesting an important role of TRPM7 in the left atrial and LV functional depression found in this cardiomyopathy. John Wiley and Sons Inc. 2016-03-23 /pmc/articles/PMC5071679/ /pubmed/27818786 http://dx.doi.org/10.1002/ehf2.12085 Text en © 2016 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of the European Society of Cardiology. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Short Communication
Ortega, Ana
Roselló‐Lletí, Esther
Tarazón, Estefanía
Gil‐Cayuela, Carolina
Lago, Francisca
González‐Juanatey, Jose‐Ramón
Martinez‐Dolz, Luis
Portolés, Manuel
Rivera, Miguel
TRPM7 is down‐regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function
title TRPM7 is down‐regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function
title_full TRPM7 is down‐regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function
title_fullStr TRPM7 is down‐regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function
title_full_unstemmed TRPM7 is down‐regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function
title_short TRPM7 is down‐regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function
title_sort trpm7 is down‐regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5071679/
https://www.ncbi.nlm.nih.gov/pubmed/27818786
http://dx.doi.org/10.1002/ehf2.12085
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