Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot

Recently, our transcriptomic analysis has identified some functional genes responsible for oil biosynthesis in developing SASK, yet miRNA-mediated regulation for SASK development and oil accumulation is poorly understood. Here, 3 representative periods of 10, 30 and 60 DAF were selected for sRNA seq...

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Autores principales: Niu, Jun, Wang, Jia, An, Jiyong, Liu, Lili, Lin, Zixin, Wang, Rui, Wang, Libing, Ma, Chao, Shi, Lingling, Lin, Shanzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5071837/
https://www.ncbi.nlm.nih.gov/pubmed/27762296
http://dx.doi.org/10.1038/srep35675
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author Niu, Jun
Wang, Jia
An, Jiyong
Liu, Lili
Lin, Zixin
Wang, Rui
Wang, Libing
Ma, Chao
Shi, Lingling
Lin, Shanzhi
author_facet Niu, Jun
Wang, Jia
An, Jiyong
Liu, Lili
Lin, Zixin
Wang, Rui
Wang, Libing
Ma, Chao
Shi, Lingling
Lin, Shanzhi
author_sort Niu, Jun
collection PubMed
description Recently, our transcriptomic analysis has identified some functional genes responsible for oil biosynthesis in developing SASK, yet miRNA-mediated regulation for SASK development and oil accumulation is poorly understood. Here, 3 representative periods of 10, 30 and 60 DAF were selected for sRNA sequencing based on the dynamic patterns of growth tendency and oil content of developing SASK. By miRNA transcriptomic analysis, we characterized 296 known and 44 novel miRNAs in developing SASK, among which 36 known and 6 novel miRNAs respond specifically to developing SASK. Importantly, we performed an integrated analysis of mRNA and miRNA transcriptome as well as qRT-PCR detection to identify some key miRNAs and their targets (miR156-SPL, miR160-ARF18, miR164-NAC1, miR171h-SCL6, miR172-AP2, miR395-AUX22B, miR530-P2C37, miR393h-TIR1/AFB2 and psi-miRn5-SnRK2A) potentially involved in developing response and hormone signaling of SASK. Our results provide new insights into the important regulatory function of cross-talk between development response and hormone signaling for SASK oil accumulation.
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spelling pubmed-50718372016-10-26 Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot Niu, Jun Wang, Jia An, Jiyong Liu, Lili Lin, Zixin Wang, Rui Wang, Libing Ma, Chao Shi, Lingling Lin, Shanzhi Sci Rep Article Recently, our transcriptomic analysis has identified some functional genes responsible for oil biosynthesis in developing SASK, yet miRNA-mediated regulation for SASK development and oil accumulation is poorly understood. Here, 3 representative periods of 10, 30 and 60 DAF were selected for sRNA sequencing based on the dynamic patterns of growth tendency and oil content of developing SASK. By miRNA transcriptomic analysis, we characterized 296 known and 44 novel miRNAs in developing SASK, among which 36 known and 6 novel miRNAs respond specifically to developing SASK. Importantly, we performed an integrated analysis of mRNA and miRNA transcriptome as well as qRT-PCR detection to identify some key miRNAs and their targets (miR156-SPL, miR160-ARF18, miR164-NAC1, miR171h-SCL6, miR172-AP2, miR395-AUX22B, miR530-P2C37, miR393h-TIR1/AFB2 and psi-miRn5-SnRK2A) potentially involved in developing response and hormone signaling of SASK. Our results provide new insights into the important regulatory function of cross-talk between development response and hormone signaling for SASK oil accumulation. Nature Publishing Group 2016-10-20 /pmc/articles/PMC5071837/ /pubmed/27762296 http://dx.doi.org/10.1038/srep35675 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Niu, Jun
Wang, Jia
An, Jiyong
Liu, Lili
Lin, Zixin
Wang, Rui
Wang, Libing
Ma, Chao
Shi, Lingling
Lin, Shanzhi
Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot
title Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot
title_full Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot
title_fullStr Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot
title_full_unstemmed Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot
title_short Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot
title_sort integrated mrna and mirna transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of siberian apricot
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5071837/
https://www.ncbi.nlm.nih.gov/pubmed/27762296
http://dx.doi.org/10.1038/srep35675
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