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Aberration-free volumetric high-speed imaging of in vivo retina

Certain topics in research and advancements in medical diagnostics may benefit from improved temporal and spatial resolution during non-invasive optical imaging of living tissue. However, so far no imaging technique can generate entirely diffraction-limited tomographic volumes with a single data acq...

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Autores principales: Hillmann, Dierck, Spahr, Hendrik, Hain, Carola, Sudkamp, Helge, Franke, Gesa, Pfäffle, Clara, Winter, Christian, Hüttmann, Gereon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5071870/
https://www.ncbi.nlm.nih.gov/pubmed/27762314
http://dx.doi.org/10.1038/srep35209
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author Hillmann, Dierck
Spahr, Hendrik
Hain, Carola
Sudkamp, Helge
Franke, Gesa
Pfäffle, Clara
Winter, Christian
Hüttmann, Gereon
author_facet Hillmann, Dierck
Spahr, Hendrik
Hain, Carola
Sudkamp, Helge
Franke, Gesa
Pfäffle, Clara
Winter, Christian
Hüttmann, Gereon
author_sort Hillmann, Dierck
collection PubMed
description Certain topics in research and advancements in medical diagnostics may benefit from improved temporal and spatial resolution during non-invasive optical imaging of living tissue. However, so far no imaging technique can generate entirely diffraction-limited tomographic volumes with a single data acquisition, if the target moves or changes rapidly, such as the human retina. Additionally, the presence of aberrations may represent further difficulties. We show that a simple interferometric setup–based on parallelized optical coherence tomography–acquires volumetric data with 10 billion voxels per second, exceeding previous imaging speeds by an order of magnitude. This allows us to computationally obtain and correct defocus and aberrations resulting in entirely diffraction-limited volumes. As demonstration, we imaged living human retina with clearly visible nerve fiber layer, small capillary networks, and photoreceptor cells. Furthermore, the technique can also obtain phase-sensitive volumes of other scattering structures at unprecedented acquisition speeds.
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spelling pubmed-50718702016-10-26 Aberration-free volumetric high-speed imaging of in vivo retina Hillmann, Dierck Spahr, Hendrik Hain, Carola Sudkamp, Helge Franke, Gesa Pfäffle, Clara Winter, Christian Hüttmann, Gereon Sci Rep Article Certain topics in research and advancements in medical diagnostics may benefit from improved temporal and spatial resolution during non-invasive optical imaging of living tissue. However, so far no imaging technique can generate entirely diffraction-limited tomographic volumes with a single data acquisition, if the target moves or changes rapidly, such as the human retina. Additionally, the presence of aberrations may represent further difficulties. We show that a simple interferometric setup–based on parallelized optical coherence tomography–acquires volumetric data with 10 billion voxels per second, exceeding previous imaging speeds by an order of magnitude. This allows us to computationally obtain and correct defocus and aberrations resulting in entirely diffraction-limited volumes. As demonstration, we imaged living human retina with clearly visible nerve fiber layer, small capillary networks, and photoreceptor cells. Furthermore, the technique can also obtain phase-sensitive volumes of other scattering structures at unprecedented acquisition speeds. Nature Publishing Group 2016-10-20 /pmc/articles/PMC5071870/ /pubmed/27762314 http://dx.doi.org/10.1038/srep35209 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hillmann, Dierck
Spahr, Hendrik
Hain, Carola
Sudkamp, Helge
Franke, Gesa
Pfäffle, Clara
Winter, Christian
Hüttmann, Gereon
Aberration-free volumetric high-speed imaging of in vivo retina
title Aberration-free volumetric high-speed imaging of in vivo retina
title_full Aberration-free volumetric high-speed imaging of in vivo retina
title_fullStr Aberration-free volumetric high-speed imaging of in vivo retina
title_full_unstemmed Aberration-free volumetric high-speed imaging of in vivo retina
title_short Aberration-free volumetric high-speed imaging of in vivo retina
title_sort aberration-free volumetric high-speed imaging of in vivo retina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5071870/
https://www.ncbi.nlm.nih.gov/pubmed/27762314
http://dx.doi.org/10.1038/srep35209
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