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Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos

Currently, chlorpyrifos (CP) and carbofuran are often applied together to control major agricultural pests in many developing countries, in most cases, they are simultaneously detected in agricultural soils. Some cost‐effective techniques are required for the remediation of combined pollution caused...

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Autores principales: Gong, Ting, Liu, Ruihua, Che, You, Xu, Xiaoqing, Zhao, Fengjie, Yu, Huilei, Song, Cunjiang, Liu, Yanping, Yang, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072195/
https://www.ncbi.nlm.nih.gov/pubmed/27418102
http://dx.doi.org/10.1111/1751-7915.12381
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author Gong, Ting
Liu, Ruihua
Che, You
Xu, Xiaoqing
Zhao, Fengjie
Yu, Huilei
Song, Cunjiang
Liu, Yanping
Yang, Chao
author_facet Gong, Ting
Liu, Ruihua
Che, You
Xu, Xiaoqing
Zhao, Fengjie
Yu, Huilei
Song, Cunjiang
Liu, Yanping
Yang, Chao
author_sort Gong, Ting
collection PubMed
description Currently, chlorpyrifos (CP) and carbofuran are often applied together to control major agricultural pests in many developing countries, in most cases, they are simultaneously detected in agricultural soils. Some cost‐effective techniques are required for the remediation of combined pollution caused by multiple pesticides. In this work, we aim at constructing a detectable recombinant microorganism with the capacity to simultaneously degrade CP and carbofuran. To achieve this purpose, CP/carbofuran hydrolase genes and gfp were integrated into the chromosome of a biosafety strain Pseudomonas putida KT2440 using a chromosomal scarless modification strategy with upp as a counter‐selectable marker. The toxicity of the hydrolysis products was significantly lower compared with the parent compounds. The recombinant strain could utilize CP or carbofuran as the sole source of carbon for growth. The inoculation of the recombinant strain to soils treated with carbofuran and CP resulted in a higher degradation rate than in noninoculated soils. Introduced green fluorescent protein can be employed as a biomarker to track the recombinant strain during bioremediation. Therefore, the recombinant strain has potential to be applied for in situ bioremediation of soil co‐contaminated with carbofuran and CP.
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spelling pubmed-50721952016-10-26 Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos Gong, Ting Liu, Ruihua Che, You Xu, Xiaoqing Zhao, Fengjie Yu, Huilei Song, Cunjiang Liu, Yanping Yang, Chao Microb Biotechnol Research Articles Currently, chlorpyrifos (CP) and carbofuran are often applied together to control major agricultural pests in many developing countries, in most cases, they are simultaneously detected in agricultural soils. Some cost‐effective techniques are required for the remediation of combined pollution caused by multiple pesticides. In this work, we aim at constructing a detectable recombinant microorganism with the capacity to simultaneously degrade CP and carbofuran. To achieve this purpose, CP/carbofuran hydrolase genes and gfp were integrated into the chromosome of a biosafety strain Pseudomonas putida KT2440 using a chromosomal scarless modification strategy with upp as a counter‐selectable marker. The toxicity of the hydrolysis products was significantly lower compared with the parent compounds. The recombinant strain could utilize CP or carbofuran as the sole source of carbon for growth. The inoculation of the recombinant strain to soils treated with carbofuran and CP resulted in a higher degradation rate than in noninoculated soils. Introduced green fluorescent protein can be employed as a biomarker to track the recombinant strain during bioremediation. Therefore, the recombinant strain has potential to be applied for in situ bioremediation of soil co‐contaminated with carbofuran and CP. John Wiley and Sons Inc. 2016-07-15 /pmc/articles/PMC5072195/ /pubmed/27418102 http://dx.doi.org/10.1111/1751-7915.12381 Text en © 2016 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Gong, Ting
Liu, Ruihua
Che, You
Xu, Xiaoqing
Zhao, Fengjie
Yu, Huilei
Song, Cunjiang
Liu, Yanping
Yang, Chao
Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos
title Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos
title_full Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos
title_fullStr Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos
title_full_unstemmed Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos
title_short Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos
title_sort engineering pseudomonas putida kt2440 for simultaneous degradation of carbofuran and chlorpyrifos
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072195/
https://www.ncbi.nlm.nih.gov/pubmed/27418102
http://dx.doi.org/10.1111/1751-7915.12381
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