Cargando…
Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos
Currently, chlorpyrifos (CP) and carbofuran are often applied together to control major agricultural pests in many developing countries, in most cases, they are simultaneously detected in agricultural soils. Some cost‐effective techniques are required for the remediation of combined pollution caused...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072195/ https://www.ncbi.nlm.nih.gov/pubmed/27418102 http://dx.doi.org/10.1111/1751-7915.12381 |
_version_ | 1782461364429651968 |
---|---|
author | Gong, Ting Liu, Ruihua Che, You Xu, Xiaoqing Zhao, Fengjie Yu, Huilei Song, Cunjiang Liu, Yanping Yang, Chao |
author_facet | Gong, Ting Liu, Ruihua Che, You Xu, Xiaoqing Zhao, Fengjie Yu, Huilei Song, Cunjiang Liu, Yanping Yang, Chao |
author_sort | Gong, Ting |
collection | PubMed |
description | Currently, chlorpyrifos (CP) and carbofuran are often applied together to control major agricultural pests in many developing countries, in most cases, they are simultaneously detected in agricultural soils. Some cost‐effective techniques are required for the remediation of combined pollution caused by multiple pesticides. In this work, we aim at constructing a detectable recombinant microorganism with the capacity to simultaneously degrade CP and carbofuran. To achieve this purpose, CP/carbofuran hydrolase genes and gfp were integrated into the chromosome of a biosafety strain Pseudomonas putida KT2440 using a chromosomal scarless modification strategy with upp as a counter‐selectable marker. The toxicity of the hydrolysis products was significantly lower compared with the parent compounds. The recombinant strain could utilize CP or carbofuran as the sole source of carbon for growth. The inoculation of the recombinant strain to soils treated with carbofuran and CP resulted in a higher degradation rate than in noninoculated soils. Introduced green fluorescent protein can be employed as a biomarker to track the recombinant strain during bioremediation. Therefore, the recombinant strain has potential to be applied for in situ bioremediation of soil co‐contaminated with carbofuran and CP. |
format | Online Article Text |
id | pubmed-5072195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50721952016-10-26 Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos Gong, Ting Liu, Ruihua Che, You Xu, Xiaoqing Zhao, Fengjie Yu, Huilei Song, Cunjiang Liu, Yanping Yang, Chao Microb Biotechnol Research Articles Currently, chlorpyrifos (CP) and carbofuran are often applied together to control major agricultural pests in many developing countries, in most cases, they are simultaneously detected in agricultural soils. Some cost‐effective techniques are required for the remediation of combined pollution caused by multiple pesticides. In this work, we aim at constructing a detectable recombinant microorganism with the capacity to simultaneously degrade CP and carbofuran. To achieve this purpose, CP/carbofuran hydrolase genes and gfp were integrated into the chromosome of a biosafety strain Pseudomonas putida KT2440 using a chromosomal scarless modification strategy with upp as a counter‐selectable marker. The toxicity of the hydrolysis products was significantly lower compared with the parent compounds. The recombinant strain could utilize CP or carbofuran as the sole source of carbon for growth. The inoculation of the recombinant strain to soils treated with carbofuran and CP resulted in a higher degradation rate than in noninoculated soils. Introduced green fluorescent protein can be employed as a biomarker to track the recombinant strain during bioremediation. Therefore, the recombinant strain has potential to be applied for in situ bioremediation of soil co‐contaminated with carbofuran and CP. John Wiley and Sons Inc. 2016-07-15 /pmc/articles/PMC5072195/ /pubmed/27418102 http://dx.doi.org/10.1111/1751-7915.12381 Text en © 2016 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Gong, Ting Liu, Ruihua Che, You Xu, Xiaoqing Zhao, Fengjie Yu, Huilei Song, Cunjiang Liu, Yanping Yang, Chao Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos |
title | Engineering Pseudomonas putida
KT2440 for simultaneous degradation of carbofuran and chlorpyrifos |
title_full | Engineering Pseudomonas putida
KT2440 for simultaneous degradation of carbofuran and chlorpyrifos |
title_fullStr | Engineering Pseudomonas putida
KT2440 for simultaneous degradation of carbofuran and chlorpyrifos |
title_full_unstemmed | Engineering Pseudomonas putida
KT2440 for simultaneous degradation of carbofuran and chlorpyrifos |
title_short | Engineering Pseudomonas putida
KT2440 for simultaneous degradation of carbofuran and chlorpyrifos |
title_sort | engineering pseudomonas putida
kt2440 for simultaneous degradation of carbofuran and chlorpyrifos |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072195/ https://www.ncbi.nlm.nih.gov/pubmed/27418102 http://dx.doi.org/10.1111/1751-7915.12381 |
work_keys_str_mv | AT gongting engineeringpseudomonasputidakt2440forsimultaneousdegradationofcarbofuranandchlorpyrifos AT liuruihua engineeringpseudomonasputidakt2440forsimultaneousdegradationofcarbofuranandchlorpyrifos AT cheyou engineeringpseudomonasputidakt2440forsimultaneousdegradationofcarbofuranandchlorpyrifos AT xuxiaoqing engineeringpseudomonasputidakt2440forsimultaneousdegradationofcarbofuranandchlorpyrifos AT zhaofengjie engineeringpseudomonasputidakt2440forsimultaneousdegradationofcarbofuranandchlorpyrifos AT yuhuilei engineeringpseudomonasputidakt2440forsimultaneousdegradationofcarbofuranandchlorpyrifos AT songcunjiang engineeringpseudomonasputidakt2440forsimultaneousdegradationofcarbofuranandchlorpyrifos AT liuyanping engineeringpseudomonasputidakt2440forsimultaneousdegradationofcarbofuranandchlorpyrifos AT yangchao engineeringpseudomonasputidakt2440forsimultaneousdegradationofcarbofuranandchlorpyrifos |