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An in Vitro Selection System for TNA
[Image: see text] (3‘-2‘)-α-l-Threose nucleic acid (TNA) is an unnatural polymer that possesses the rare ability to base-pair with RNA, DNA, and itself. This feature, coupled with its chemical simplicity, makes TNA of interest as a possible progenitor of RNA during the early history of life. To eval...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072288/ https://www.ncbi.nlm.nih.gov/pubmed/15740086 http://dx.doi.org/10.1021/ja045364w |
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author | Ichida, Justin K. Zou, Keyong Horhota, Allen Yu, Biao McLaughlin, Larry W. Szostak, Jack W. |
author_facet | Ichida, Justin K. Zou, Keyong Horhota, Allen Yu, Biao McLaughlin, Larry W. Szostak, Jack W. |
author_sort | Ichida, Justin K. |
collection | PubMed |
description | [Image: see text] (3‘-2‘)-α-l-Threose nucleic acid (TNA) is an unnatural polymer that possesses the rare ability to base-pair with RNA, DNA, and itself. This feature, coupled with its chemical simplicity, makes TNA of interest as a possible progenitor of RNA during the early history of life. To evaluate the functional potential of TNA, we have developed a system for the in vitro selection of TNA. We identified the Therminator DNA polymerase as a remarkably efficient DNA-dependent TNA polymerase capable of polymerizing more than 50 tNTPs. We have also developed a method of covalently linking a DNA template to the TNA strand that it encodes, thus obviating the need for a TNA-dependent DNA polymerase during cycles of selection. |
format | Online Article Text |
id | pubmed-5072288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-50722882016-10-21 An in Vitro Selection System for TNA Ichida, Justin K. Zou, Keyong Horhota, Allen Yu, Biao McLaughlin, Larry W. Szostak, Jack W. J Am Chem Soc [Image: see text] (3‘-2‘)-α-l-Threose nucleic acid (TNA) is an unnatural polymer that possesses the rare ability to base-pair with RNA, DNA, and itself. This feature, coupled with its chemical simplicity, makes TNA of interest as a possible progenitor of RNA during the early history of life. To evaluate the functional potential of TNA, we have developed a system for the in vitro selection of TNA. We identified the Therminator DNA polymerase as a remarkably efficient DNA-dependent TNA polymerase capable of polymerizing more than 50 tNTPs. We have also developed a method of covalently linking a DNA template to the TNA strand that it encodes, thus obviating the need for a TNA-dependent DNA polymerase during cycles of selection. American Chemical Society 2005-02-10 2005-03-09 /pmc/articles/PMC5072288/ /pubmed/15740086 http://dx.doi.org/10.1021/ja045364w Text en Copyright © 2005 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Ichida, Justin K. Zou, Keyong Horhota, Allen Yu, Biao McLaughlin, Larry W. Szostak, Jack W. An in Vitro Selection System for TNA |
title | An in Vitro Selection System for TNA |
title_full | An in Vitro Selection System for TNA |
title_fullStr | An in Vitro Selection System for TNA |
title_full_unstemmed | An in Vitro Selection System for TNA |
title_short | An in Vitro Selection System for TNA |
title_sort | in vitro selection system for tna |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072288/ https://www.ncbi.nlm.nih.gov/pubmed/15740086 http://dx.doi.org/10.1021/ja045364w |
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