Genomic Disruption of VEGF-A Expression in Human Retinal Pigment Epithelial Cells Using CRISPR-Cas9 Endonuclease

PURPOSE: To employ type II clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 endonuclease to suppress ocular angiogenesis by genomic disruption of VEGF-A in human RPE cells. METHODS: CRISPR sequences targeting exon 1 of human VEGF-A were computationally identified based on pred...

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Detalles Bibliográficos
Autores principales: Yiu, Glenn, Tieu, Eric, Nguyen, Anthony T., Wong, Brittany, Smit-McBride, Zeljka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2016
Materias:
Retinal Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072539/
https://www.ncbi.nlm.nih.gov/pubmed/27768202
http://dx.doi.org/10.1167/iovs.16-20296

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072539/
https://www.ncbi.nlm.nih.gov/pubmed/27768202
http://dx.doi.org/10.1167/iovs.16-20296

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