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RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress
Plants respond to stress through metabolic and morphological changes that increase their ability to survive and grow. To this end, several transcription factor families are responsible for transmitting the signals that are required for these changes. Here, we studied the transcription factor superfa...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072549/ https://www.ncbi.nlm.nih.gov/pubmed/27764197 http://dx.doi.org/10.1371/journal.pone.0165030 |
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author | Figueroa-Yañez, Luis Pereira-Santana, Alejandro Arroyo-Herrera, Ana Rodriguez-Corona, Ulises Sanchez-Teyer, Felipe Espadas-Alcocer, Jorge Espadas-Gil, Francisco Barredo-Pool, Felipe Castaño, Enrique Rodriguez-Zapata, Luis Carlos |
author_facet | Figueroa-Yañez, Luis Pereira-Santana, Alejandro Arroyo-Herrera, Ana Rodriguez-Corona, Ulises Sanchez-Teyer, Felipe Espadas-Alcocer, Jorge Espadas-Gil, Francisco Barredo-Pool, Felipe Castaño, Enrique Rodriguez-Zapata, Luis Carlos |
author_sort | Figueroa-Yañez, Luis |
collection | PubMed |
description | Plants respond to stress through metabolic and morphological changes that increase their ability to survive and grow. To this end, several transcription factor families are responsible for transmitting the signals that are required for these changes. Here, we studied the transcription factor superfamily AP2/ERF, particularly, RAP2.4 from Carica papaya cv. Maradol. We isolated four genes (CpRap2.4a, CpRAap2.4b, CpRap2.1 and CpRap2.10), and an in silico analysis showed that the four genes encode proteins that contain a conserved APETALA2 (AP2) domain located within group I and II transcription factors of the AP2/ERF superfamily. Semiquantitative PCR experiments indicated that each CpRap2 gene is differentially expressed under stress conditions, such as extreme temperatures. Moreover, genetic transformants of tobacco plants overexpressing CpRap2.4a and CpRap2.4b genes show a high level of tolerance to cold and heat stress compared to non-transformed plants. Confocal microscopy analysis of tobacco transgenic plants showed that CpRAP2.4a and CpRAP2.4b proteins were mainly localized to the nuclei of cells from the leaves and roots and also in the sieve elements. Moreover, the movement of CpRap2.4a RNA in tobacco grafting was analyzed. Our results indicate that CpRap2.4a and CpRap2.4b RNA in the papaya tree have a functional role in the response to stress conditions such as exposure to extreme temperatures via direct translation outside the parental RNA cell. |
format | Online Article Text |
id | pubmed-5072549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50725492016-10-27 RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress Figueroa-Yañez, Luis Pereira-Santana, Alejandro Arroyo-Herrera, Ana Rodriguez-Corona, Ulises Sanchez-Teyer, Felipe Espadas-Alcocer, Jorge Espadas-Gil, Francisco Barredo-Pool, Felipe Castaño, Enrique Rodriguez-Zapata, Luis Carlos PLoS One Research Article Plants respond to stress through metabolic and morphological changes that increase their ability to survive and grow. To this end, several transcription factor families are responsible for transmitting the signals that are required for these changes. Here, we studied the transcription factor superfamily AP2/ERF, particularly, RAP2.4 from Carica papaya cv. Maradol. We isolated four genes (CpRap2.4a, CpRAap2.4b, CpRap2.1 and CpRap2.10), and an in silico analysis showed that the four genes encode proteins that contain a conserved APETALA2 (AP2) domain located within group I and II transcription factors of the AP2/ERF superfamily. Semiquantitative PCR experiments indicated that each CpRap2 gene is differentially expressed under stress conditions, such as extreme temperatures. Moreover, genetic transformants of tobacco plants overexpressing CpRap2.4a and CpRap2.4b genes show a high level of tolerance to cold and heat stress compared to non-transformed plants. Confocal microscopy analysis of tobacco transgenic plants showed that CpRAP2.4a and CpRAP2.4b proteins were mainly localized to the nuclei of cells from the leaves and roots and also in the sieve elements. Moreover, the movement of CpRap2.4a RNA in tobacco grafting was analyzed. Our results indicate that CpRap2.4a and CpRap2.4b RNA in the papaya tree have a functional role in the response to stress conditions such as exposure to extreme temperatures via direct translation outside the parental RNA cell. Public Library of Science 2016-10-20 /pmc/articles/PMC5072549/ /pubmed/27764197 http://dx.doi.org/10.1371/journal.pone.0165030 Text en © 2016 Figueroa-Yañez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Figueroa-Yañez, Luis Pereira-Santana, Alejandro Arroyo-Herrera, Ana Rodriguez-Corona, Ulises Sanchez-Teyer, Felipe Espadas-Alcocer, Jorge Espadas-Gil, Francisco Barredo-Pool, Felipe Castaño, Enrique Rodriguez-Zapata, Luis Carlos RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress |
title | RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress |
title_full | RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress |
title_fullStr | RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress |
title_full_unstemmed | RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress |
title_short | RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress |
title_sort | rap2.4a is transported through the phloem to regulate cold and heat tolerance in papaya tree (carica papaya cv. maradol): implications for protection against abiotic stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072549/ https://www.ncbi.nlm.nih.gov/pubmed/27764197 http://dx.doi.org/10.1371/journal.pone.0165030 |
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