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Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages

Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny a...

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Autores principales: Park, Kun Taek, ElNaggar, Mahmoud M., Abdellrazeq, Gaber S., Bannantine, John P., Mack, Victoria, Fry, Lindsay M., Davis, William C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072659/
https://www.ncbi.nlm.nih.gov/pubmed/27764236
http://dx.doi.org/10.1371/journal.pone.0165247
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author Park, Kun Taek
ElNaggar, Mahmoud M.
Abdellrazeq, Gaber S.
Bannantine, John P.
Mack, Victoria
Fry, Lindsay M.
Davis, William C.
author_facet Park, Kun Taek
ElNaggar, Mahmoud M.
Abdellrazeq, Gaber S.
Bannantine, John P.
Mack, Victoria
Fry, Lindsay M.
Davis, William C.
author_sort Park, Kun Taek
collection PubMed
description Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny and function: conventional DC (cDC1 and cDC2), plasmacytoid DC (pDC), and monocyte derived DC (MoDC). DC of Artiodactyla (pigs and ruminants) can also be sub-classified using this system, allowing direct functional and phenotypic comparison of MoDC and other DC subsets trafficking in blood (bDC). Because of the high volume of blood collections required to study DC, cattle offer the best opportunity to further our understanding of bDC and MoDC function in an outbred large animal species. As reported here, phenotyping DC using a monoclonal antibody (mAb) to CD209 revealed CD209 is expressed on the major myeloid population of DC present in blood and MoDC, providing a phenotypic link between these two subsets. Additionally, the present study demonstrates that CD209 is also expressed on monocyte derived macrophages (MoΦ). Functional analysis revealed each of these populations can take up and process antigens (Ags), present them to CD4 and CD8 T cells, and elicit a T-cell recall response. Thus, bDC, MoDC, and MoΦ pulsed with pathogens or candidate vaccine antigens can be used to study factors that modulate DC-driven T-cell priming and differentiation ex vivo.
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spelling pubmed-50726592016-10-27 Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages Park, Kun Taek ElNaggar, Mahmoud M. Abdellrazeq, Gaber S. Bannantine, John P. Mack, Victoria Fry, Lindsay M. Davis, William C. PLoS One Research Article Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny and function: conventional DC (cDC1 and cDC2), plasmacytoid DC (pDC), and monocyte derived DC (MoDC). DC of Artiodactyla (pigs and ruminants) can also be sub-classified using this system, allowing direct functional and phenotypic comparison of MoDC and other DC subsets trafficking in blood (bDC). Because of the high volume of blood collections required to study DC, cattle offer the best opportunity to further our understanding of bDC and MoDC function in an outbred large animal species. As reported here, phenotyping DC using a monoclonal antibody (mAb) to CD209 revealed CD209 is expressed on the major myeloid population of DC present in blood and MoDC, providing a phenotypic link between these two subsets. Additionally, the present study demonstrates that CD209 is also expressed on monocyte derived macrophages (MoΦ). Functional analysis revealed each of these populations can take up and process antigens (Ags), present them to CD4 and CD8 T cells, and elicit a T-cell recall response. Thus, bDC, MoDC, and MoΦ pulsed with pathogens or candidate vaccine antigens can be used to study factors that modulate DC-driven T-cell priming and differentiation ex vivo. Public Library of Science 2016-10-20 /pmc/articles/PMC5072659/ /pubmed/27764236 http://dx.doi.org/10.1371/journal.pone.0165247 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Park, Kun Taek
ElNaggar, Mahmoud M.
Abdellrazeq, Gaber S.
Bannantine, John P.
Mack, Victoria
Fry, Lindsay M.
Davis, William C.
Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages
title Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages
title_full Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages
title_fullStr Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages
title_full_unstemmed Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages
title_short Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages
title_sort phenotype and function of cd209(+) bovine blood dendritic cells, monocyte-derived-dendritic cells and monocyte-derived macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072659/
https://www.ncbi.nlm.nih.gov/pubmed/27764236
http://dx.doi.org/10.1371/journal.pone.0165247
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