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Genome-Wide Meta-Analysis of Sciatica in Finnish Population

Sciatica or the sciatic syndrome is a common and often disabling low back disorder in the working-age population. It has a relatively high heritability but poorly understood molecular mechanisms. The Finnish population is a genetic isolate where small founder population and bottleneck events have le...

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Autores principales: Lemmelä, Susanna, Solovieva, Svetlana, Shiri, Rahman, Benner, Christian, Heliövaara, Markku, Kettunen, Johannes, Anttila, Verneri, Ripatti, Samuli, Perola, Markus, Seppälä, Ilkka, Juonala, Markus, Kähönen, Mika, Salomaa, Veikko, Viikari, Jorma, Raitakari, Olli T., Lehtimäki, Terho, Palotie, Aarno, Viikari-Juntura, Eira, Husgafvel-Pursiainen, Kirsti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072673/
https://www.ncbi.nlm.nih.gov/pubmed/27764105
http://dx.doi.org/10.1371/journal.pone.0163877
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author Lemmelä, Susanna
Solovieva, Svetlana
Shiri, Rahman
Benner, Christian
Heliövaara, Markku
Kettunen, Johannes
Anttila, Verneri
Ripatti, Samuli
Perola, Markus
Seppälä, Ilkka
Juonala, Markus
Kähönen, Mika
Salomaa, Veikko
Viikari, Jorma
Raitakari, Olli T.
Lehtimäki, Terho
Palotie, Aarno
Viikari-Juntura, Eira
Husgafvel-Pursiainen, Kirsti
author_facet Lemmelä, Susanna
Solovieva, Svetlana
Shiri, Rahman
Benner, Christian
Heliövaara, Markku
Kettunen, Johannes
Anttila, Verneri
Ripatti, Samuli
Perola, Markus
Seppälä, Ilkka
Juonala, Markus
Kähönen, Mika
Salomaa, Veikko
Viikari, Jorma
Raitakari, Olli T.
Lehtimäki, Terho
Palotie, Aarno
Viikari-Juntura, Eira
Husgafvel-Pursiainen, Kirsti
author_sort Lemmelä, Susanna
collection PubMed
description Sciatica or the sciatic syndrome is a common and often disabling low back disorder in the working-age population. It has a relatively high heritability but poorly understood molecular mechanisms. The Finnish population is a genetic isolate where small founder population and bottleneck events have led to enrichment of certain rare and low frequency variants. We performed here the first genome-wide association (GWAS) and meta-analysis of sciatica. The meta-analysis was conducted across two GWAS covering 291 Finnish sciatica cases and 3671 controls genotyped and imputed at 7.7 million autosomal variants. The most promising loci (p<1x10(-6)) were replicated in 776 Finnish sciatica patients and 18,489 controls. We identified five intragenic variants, with relatively low frequencies, at two novel loci associated with sciatica at genome-wide significance. These included chr9:14344410:I (rs71321981) at 9p22.3 (NFIB gene; p = 1.30x10(-8), MAF = 0.08) and four variants at 15q21.2: rs145901849, rs80035109, rs190200374 and rs117458827 (MYO5A; p = 1.34x10(-8), MAF = 0.06; p = 2.32x10(-8), MAF = 0.07; p = 3.85x10(-8), MAF = 0.06; p = 4.78x10(-8), MAF = 0.07, respectively). The most significant association in the meta-analysis, a single base insertion rs71321981 within the regulatory region of the transcription factor NFIB, replicated in an independent Finnish population sample (p = 0.04). Despite identifying 15q21.2 as a promising locus, we were not able to replicate it. It was differentiated; the lead variants within 15q21.2 were more frequent in Finland (6–7%) than in other European populations (1–2%). Imputation accuracies of the three significantly associated variants (chr9:14344410:I, rs190200374, and rs80035109) were validated by genotyping. In summary, our results suggest a novel locus, 9p22.3 (NFIB), which may be involved in susceptibility to sciatica. In addition, another locus, 15q21.2, emerged as a promising one, but failed to replicate.
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spelling pubmed-50726732016-10-27 Genome-Wide Meta-Analysis of Sciatica in Finnish Population Lemmelä, Susanna Solovieva, Svetlana Shiri, Rahman Benner, Christian Heliövaara, Markku Kettunen, Johannes Anttila, Verneri Ripatti, Samuli Perola, Markus Seppälä, Ilkka Juonala, Markus Kähönen, Mika Salomaa, Veikko Viikari, Jorma Raitakari, Olli T. Lehtimäki, Terho Palotie, Aarno Viikari-Juntura, Eira Husgafvel-Pursiainen, Kirsti PLoS One Research Article Sciatica or the sciatic syndrome is a common and often disabling low back disorder in the working-age population. It has a relatively high heritability but poorly understood molecular mechanisms. The Finnish population is a genetic isolate where small founder population and bottleneck events have led to enrichment of certain rare and low frequency variants. We performed here the first genome-wide association (GWAS) and meta-analysis of sciatica. The meta-analysis was conducted across two GWAS covering 291 Finnish sciatica cases and 3671 controls genotyped and imputed at 7.7 million autosomal variants. The most promising loci (p<1x10(-6)) were replicated in 776 Finnish sciatica patients and 18,489 controls. We identified five intragenic variants, with relatively low frequencies, at two novel loci associated with sciatica at genome-wide significance. These included chr9:14344410:I (rs71321981) at 9p22.3 (NFIB gene; p = 1.30x10(-8), MAF = 0.08) and four variants at 15q21.2: rs145901849, rs80035109, rs190200374 and rs117458827 (MYO5A; p = 1.34x10(-8), MAF = 0.06; p = 2.32x10(-8), MAF = 0.07; p = 3.85x10(-8), MAF = 0.06; p = 4.78x10(-8), MAF = 0.07, respectively). The most significant association in the meta-analysis, a single base insertion rs71321981 within the regulatory region of the transcription factor NFIB, replicated in an independent Finnish population sample (p = 0.04). Despite identifying 15q21.2 as a promising locus, we were not able to replicate it. It was differentiated; the lead variants within 15q21.2 were more frequent in Finland (6–7%) than in other European populations (1–2%). Imputation accuracies of the three significantly associated variants (chr9:14344410:I, rs190200374, and rs80035109) were validated by genotyping. In summary, our results suggest a novel locus, 9p22.3 (NFIB), which may be involved in susceptibility to sciatica. In addition, another locus, 15q21.2, emerged as a promising one, but failed to replicate. Public Library of Science 2016-10-20 /pmc/articles/PMC5072673/ /pubmed/27764105 http://dx.doi.org/10.1371/journal.pone.0163877 Text en © 2016 Lemmelä et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lemmelä, Susanna
Solovieva, Svetlana
Shiri, Rahman
Benner, Christian
Heliövaara, Markku
Kettunen, Johannes
Anttila, Verneri
Ripatti, Samuli
Perola, Markus
Seppälä, Ilkka
Juonala, Markus
Kähönen, Mika
Salomaa, Veikko
Viikari, Jorma
Raitakari, Olli T.
Lehtimäki, Terho
Palotie, Aarno
Viikari-Juntura, Eira
Husgafvel-Pursiainen, Kirsti
Genome-Wide Meta-Analysis of Sciatica in Finnish Population
title Genome-Wide Meta-Analysis of Sciatica in Finnish Population
title_full Genome-Wide Meta-Analysis of Sciatica in Finnish Population
title_fullStr Genome-Wide Meta-Analysis of Sciatica in Finnish Population
title_full_unstemmed Genome-Wide Meta-Analysis of Sciatica in Finnish Population
title_short Genome-Wide Meta-Analysis of Sciatica in Finnish Population
title_sort genome-wide meta-analysis of sciatica in finnish population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072673/
https://www.ncbi.nlm.nih.gov/pubmed/27764105
http://dx.doi.org/10.1371/journal.pone.0163877
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