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Improving the Thermostability of Acidic Pullulanase from Bacillus naganoensis by Rational Design
Pullulanase (EC 3.2.1.41) plays an important role in the specific hydrolysis of branch points in amylopectin. Enhancing its thermostability is required for its industrial application. In this study, rational protein design was used to improve the thermostability of PulB from Bacillus naganoensis (AB...
Autores principales: | Chang, Meihui, Chu, Xiaoyu, Lv, Jinzhi, Li, Qingbin, Tian, Jian, Wu, Ningfeng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072709/ https://www.ncbi.nlm.nih.gov/pubmed/27764201 http://dx.doi.org/10.1371/journal.pone.0165006 |
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