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Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes
A whole-organ regeneration approach, using a decellularised xenogeneic liver as a scaffold for the construction of a transplantable liver was recently reported. Deriving suitable scaffolds was the first step towards clinical application; however, effective recellularisation remains to be achieved. T...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5073336/ https://www.ncbi.nlm.nih.gov/pubmed/27767181 http://dx.doi.org/10.1038/srep35887 |
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author | Ogiso, Satoshi Yasuchika, Kentaro Fukumitsu, Ken Ishii, Takamichi Kojima, Hidenobu Miyauchi, Yuya Yamaoka, Ryoya Komori, Junji Katayama, Hokahiro Kawai, Takayuki Yoshitoshi, Elena Yukie Kita, Sadahiko Yasuda, Katsutaro Uemoto, Shinji |
author_facet | Ogiso, Satoshi Yasuchika, Kentaro Fukumitsu, Ken Ishii, Takamichi Kojima, Hidenobu Miyauchi, Yuya Yamaoka, Ryoya Komori, Junji Katayama, Hokahiro Kawai, Takayuki Yoshitoshi, Elena Yukie Kita, Sadahiko Yasuda, Katsutaro Uemoto, Shinji |
author_sort | Ogiso, Satoshi |
collection | PubMed |
description | A whole-organ regeneration approach, using a decellularised xenogeneic liver as a scaffold for the construction of a transplantable liver was recently reported. Deriving suitable scaffolds was the first step towards clinical application; however, effective recellularisation remains to be achieved. This report presents a strategy for the improvement of the recellularisation process, using novel cell-seeding technique and cell source. We evaluated recellularised liver grafts repopulated through the portal vein or the biliary duct with mice adult hepatocytes or E14.5 foetal hepatocytes. More than 80% of the cells seeded through the biliary tree entered the parenchyma beyond the ductule-lining matrix barrier and distributed throughout the liver lobule. In contrast, about 20% of the cells seeded through the portal tree entered the parenchyma. The gene expression levels of foetal hepatocyte albumin, glucose 6-phosphatase, transferrin, cytokeratin 19, and gamma-glutamyl transpeptidase were increased in three-dimensional cultures in the native liver-derived scaffolds, and the activation of liver detoxification enzymes and formation of biliary duct-like structures were supported. The metabolic functions of liver grafts recellularised with different cell types were similar. These results suggest that biliary tree cell-seeding approach is promising, and that liver progenitor cells represent a good cell source candidate. |
format | Online Article Text |
id | pubmed-5073336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50733362016-10-26 Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes Ogiso, Satoshi Yasuchika, Kentaro Fukumitsu, Ken Ishii, Takamichi Kojima, Hidenobu Miyauchi, Yuya Yamaoka, Ryoya Komori, Junji Katayama, Hokahiro Kawai, Takayuki Yoshitoshi, Elena Yukie Kita, Sadahiko Yasuda, Katsutaro Uemoto, Shinji Sci Rep Article A whole-organ regeneration approach, using a decellularised xenogeneic liver as a scaffold for the construction of a transplantable liver was recently reported. Deriving suitable scaffolds was the first step towards clinical application; however, effective recellularisation remains to be achieved. This report presents a strategy for the improvement of the recellularisation process, using novel cell-seeding technique and cell source. We evaluated recellularised liver grafts repopulated through the portal vein or the biliary duct with mice adult hepatocytes or E14.5 foetal hepatocytes. More than 80% of the cells seeded through the biliary tree entered the parenchyma beyond the ductule-lining matrix barrier and distributed throughout the liver lobule. In contrast, about 20% of the cells seeded through the portal tree entered the parenchyma. The gene expression levels of foetal hepatocyte albumin, glucose 6-phosphatase, transferrin, cytokeratin 19, and gamma-glutamyl transpeptidase were increased in three-dimensional cultures in the native liver-derived scaffolds, and the activation of liver detoxification enzymes and formation of biliary duct-like structures were supported. The metabolic functions of liver grafts recellularised with different cell types were similar. These results suggest that biliary tree cell-seeding approach is promising, and that liver progenitor cells represent a good cell source candidate. Nature Publishing Group 2016-10-21 /pmc/articles/PMC5073336/ /pubmed/27767181 http://dx.doi.org/10.1038/srep35887 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ogiso, Satoshi Yasuchika, Kentaro Fukumitsu, Ken Ishii, Takamichi Kojima, Hidenobu Miyauchi, Yuya Yamaoka, Ryoya Komori, Junji Katayama, Hokahiro Kawai, Takayuki Yoshitoshi, Elena Yukie Kita, Sadahiko Yasuda, Katsutaro Uemoto, Shinji Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes |
title | Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes |
title_full | Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes |
title_fullStr | Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes |
title_full_unstemmed | Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes |
title_short | Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes |
title_sort | efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5073336/ https://www.ncbi.nlm.nih.gov/pubmed/27767181 http://dx.doi.org/10.1038/srep35887 |
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