A novel methanol-free Pichia pastoris system for recombinant protein expression

BACKGROUND: As one of the most popular expression systems, recombinant protein expression in Pichia pastoris relies on the AOX1 promoter (P(AOX1)) which is strongly induced by methanol. However, the toxic and inflammatory nature of methanol restricts its application, especially in edible and medical...

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Detalles Bibliográficos
Autores principales: Shen, Wei, Xue, Ying, Liu, Yiqi, Kong, Chuixing, Wang, Xiaolong, Huang, Mengmeng, Cai, Menghao, Zhou, Xiangshan, Zhang, Yuanxing, Zhou, Mian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5073731/
https://www.ncbi.nlm.nih.gov/pubmed/27769297
http://dx.doi.org/10.1186/s12934-016-0578-4

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