Cargando…
Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties
BACKGROUND: In infected periapical tissues, Enterococcus faecalis is one of the most common dominant bacteria. Chlorhexidine has been proved to show strong antibacterial ability against E. faecalis but is ineffective in promoting mineralization for tissues around root apex. Mesoporous calcium-silica...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5073856/ https://www.ncbi.nlm.nih.gov/pubmed/27769275 http://dx.doi.org/10.1186/s12951-016-0224-7 |
_version_ | 1782461644372180992 |
---|---|
author | Fan, Wei Li, Yanyun Sun, Qing Ma, Tengjiao Fan, Bing |
author_facet | Fan, Wei Li, Yanyun Sun, Qing Ma, Tengjiao Fan, Bing |
author_sort | Fan, Wei |
collection | PubMed |
description | BACKGROUND: In infected periapical tissues, Enterococcus faecalis is one of the most common dominant bacteria. Chlorhexidine has been proved to show strong antibacterial ability against E. faecalis but is ineffective in promoting mineralization for tissues around root apex. Mesoporous calcium-silicate nanoparticles are newly synthesized biomaterials with excellent ability to promote mineralization and carry-release bioactive molecules in a controlled manner. In this study, mesoporous calcium-silicate nanoparticles were functionalized with chlorhexidine and their releasing profile, antibacterial ability, effect on cell proliferation and in vitro mineralization property were evaluated. RESULTS: The chlorhexidine was successfully incorporated into mesoporous calcium-silicate nanoparticles by a mixing-coupling method. The new material could release chlorhexidine as well as Ca(2+) and SiO(3) (2−) in a sustained manner with an alkaline pH value under different conditions. The antimicrobial ability against planktonic E. faecalis was dramatically improved after chlorhexidine incorporation. The nanoparticles with chlorhexidine showed no negative effect on cell proliferation with low concentrations. On dentin slices, the new synthesized material demonstrated a similar inhibitory effect on E. faecalis as the chlorhexidine. After being immersed in SBF for 9 days, numerous apatite crystals could be observed on surfaces of the material tablets. CONCLUSIONS: Mesoporous calcium-silicate nanoparticles loaded with chlorhexidine exhibited release of ions and chlorhexidine, low cytotoxicity, excellent antibacterial ability and in vitro mineralization. This material could be developed into a new effective intra-canal medication in dentistry or a new bone defect filling material for infected bone defects. |
format | Online Article Text |
id | pubmed-5073856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-50738562016-10-26 Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties Fan, Wei Li, Yanyun Sun, Qing Ma, Tengjiao Fan, Bing J Nanobiotechnology Research BACKGROUND: In infected periapical tissues, Enterococcus faecalis is one of the most common dominant bacteria. Chlorhexidine has been proved to show strong antibacterial ability against E. faecalis but is ineffective in promoting mineralization for tissues around root apex. Mesoporous calcium-silicate nanoparticles are newly synthesized biomaterials with excellent ability to promote mineralization and carry-release bioactive molecules in a controlled manner. In this study, mesoporous calcium-silicate nanoparticles were functionalized with chlorhexidine and their releasing profile, antibacterial ability, effect on cell proliferation and in vitro mineralization property were evaluated. RESULTS: The chlorhexidine was successfully incorporated into mesoporous calcium-silicate nanoparticles by a mixing-coupling method. The new material could release chlorhexidine as well as Ca(2+) and SiO(3) (2−) in a sustained manner with an alkaline pH value under different conditions. The antimicrobial ability against planktonic E. faecalis was dramatically improved after chlorhexidine incorporation. The nanoparticles with chlorhexidine showed no negative effect on cell proliferation with low concentrations. On dentin slices, the new synthesized material demonstrated a similar inhibitory effect on E. faecalis as the chlorhexidine. After being immersed in SBF for 9 days, numerous apatite crystals could be observed on surfaces of the material tablets. CONCLUSIONS: Mesoporous calcium-silicate nanoparticles loaded with chlorhexidine exhibited release of ions and chlorhexidine, low cytotoxicity, excellent antibacterial ability and in vitro mineralization. This material could be developed into a new effective intra-canal medication in dentistry or a new bone defect filling material for infected bone defects. BioMed Central 2016-10-21 /pmc/articles/PMC5073856/ /pubmed/27769275 http://dx.doi.org/10.1186/s12951-016-0224-7 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Fan, Wei Li, Yanyun Sun, Qing Ma, Tengjiao Fan, Bing Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties |
title | Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties |
title_full | Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties |
title_fullStr | Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties |
title_full_unstemmed | Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties |
title_short | Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties |
title_sort | calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- enterococcus faecalis and mineralization properties |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5073856/ https://www.ncbi.nlm.nih.gov/pubmed/27769275 http://dx.doi.org/10.1186/s12951-016-0224-7 |
work_keys_str_mv | AT fanwei calciumsilicatemesoporousnanoparticlesloadedwithchlorhexidineforbothantienterococcusfaecalisandmineralizationproperties AT liyanyun calciumsilicatemesoporousnanoparticlesloadedwithchlorhexidineforbothantienterococcusfaecalisandmineralizationproperties AT sunqing calciumsilicatemesoporousnanoparticlesloadedwithchlorhexidineforbothantienterococcusfaecalisandmineralizationproperties AT matengjiao calciumsilicatemesoporousnanoparticlesloadedwithchlorhexidineforbothantienterococcusfaecalisandmineralizationproperties AT fanbing calciumsilicatemesoporousnanoparticlesloadedwithchlorhexidineforbothantienterococcusfaecalisandmineralizationproperties |