Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach

We determined differences in the protein abundance among two isogenic strains of Mycobacterium tuberculosis (Mtb) with different Isoniazid (INH) susceptibility profiles. The strains were isolated from a pulmonary tuberculosis patient before and after drug treatment. LC‐MS/MS analysis identified 46 M...

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Autores principales: Nieto R, Luisa María, Mehaffy, Carolina, Dobos, Karen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5074239/
https://www.ncbi.nlm.nih.gov/pubmed/26929115
http://dx.doi.org/10.1002/pmic.201500403
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author Nieto R, Luisa María
Mehaffy, Carolina
Dobos, Karen M.
author_facet Nieto R, Luisa María
Mehaffy, Carolina
Dobos, Karen M.
author_sort Nieto R, Luisa María
collection PubMed
description We determined differences in the protein abundance among two isogenic strains of Mycobacterium tuberculosis (Mtb) with different Isoniazid (INH) susceptibility profiles. The strains were isolated from a pulmonary tuberculosis patient before and after drug treatment. LC‐MS/MS analysis identified 46 Mtb proteins with altered abundance after INH resistance acquisition. Protein abundance comparisons were done evaluating the different bacterial cellular fractions (membrane, cytosol, cell wall and secreted proteins). MS data have been deposited to the ProteomeXchange with identifier PXD002986.
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spelling pubmed-50742392016-11-04 Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach Nieto R, Luisa María Mehaffy, Carolina Dobos, Karen M. Proteomics Microbiology We determined differences in the protein abundance among two isogenic strains of Mycobacterium tuberculosis (Mtb) with different Isoniazid (INH) susceptibility profiles. The strains were isolated from a pulmonary tuberculosis patient before and after drug treatment. LC‐MS/MS analysis identified 46 Mtb proteins with altered abundance after INH resistance acquisition. Protein abundance comparisons were done evaluating the different bacterial cellular fractions (membrane, cytosol, cell wall and secreted proteins). MS data have been deposited to the ProteomeXchange with identifier PXD002986. John Wiley and Sons Inc. 2016-04-13 2016-05 /pmc/articles/PMC5074239/ /pubmed/26929115 http://dx.doi.org/10.1002/pmic.201500403 Text en © 2016 The Authors. Proteomics Published by Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Microbiology
Nieto R, Luisa María
Mehaffy, Carolina
Dobos, Karen M.
Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach
title Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach
title_full Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach
title_fullStr Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach
title_full_unstemmed Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach
title_short Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach
title_sort comparing isogenic strains of beijing genotype mycobacterium tuberculosis after acquisition of isoniazid resistance: a proteomics approach
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5074239/
https://www.ncbi.nlm.nih.gov/pubmed/26929115
http://dx.doi.org/10.1002/pmic.201500403
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AT doboskarenm comparingisogenicstrainsofbeijinggenotypemycobacteriumtuberculosisafteracquisitionofisoniazidresistanceaproteomicsapproach

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