Cargando…
A Microplate-Based Nonradioactive Protein Synthesis Assay: Application to TRAIL Sensitization by Protein Synthesis Inhibitors
Non-radioactive assays based on incorporation of puromycin into newly synthesized proteins and subsequent detection using anti-puromycin antibodies have been previously reported and well-validated. To develop a moderate- to high-throughput assay, an adaptation is here described wherein cells are pur...
Autor principal: | Henrich, Curtis J. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5074477/ https://www.ncbi.nlm.nih.gov/pubmed/27768779 http://dx.doi.org/10.1371/journal.pone.0165192 |
Ejemplares similares
-
Methods in nonradioactive detection
Publicado: (1993) -
Higher Sensitivity and Earlier Identification of Celiac Disease Autoimmunity by a Nonradioactive Assay for Transglutaminase Autoantibodies
por: Zhao, Zhiyuan, et al.
Publicado: (2016) -
Glucose Metabolic Trapping in Mouse Arteries: Nonradioactive Assay of Atherosclerotic Plaque Inflammation Applicable to Drug Discovery
por: Conway, Richard G., et al.
Publicado: (2012) -
A Nonradioactive Fluorimetric SPE-Based Ceramide Kinase Assay Using NBD-C(6)-Ceramide
por: Van Overloop, Helena, et al.
Publicado: (2012) -
Detection of thermal shift in cellular Keap1 by protein-protein interaction inhibitors using immunoblot- and fluorescence microplate-based assays
por: Dayalan Naidu, Sharadha, et al.
Publicado: (2022)