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Diagnostic moléculaire du Cytomégalovirus (CMV), de l’herpès virus humain de type 6 (HHV6) et d’Epstein-Barr virus (EBV) par PCR en temps réel chez les femmes enceintes VIH séropositives et séronégatives à Ouagadougou, Burkina Faso
INTRODUCTION: Herpesvirus EBV, CMV and HHV-6 are viruses that evolve based on pandemic modeling and are responsible for congenital infections causing severe sequelae in infants. This study aims to determine the prevalence of CMV, EBV and HHV-6 among HIV (+) and HIV (-) pregnant women in Ouagadougou....
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The African Field Epidemiology Network
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075482/ https://www.ncbi.nlm.nih.gov/pubmed/27800078 http://dx.doi.org/10.11604/pamj.2016.24.223.9406 |
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author | Ouedraogo, Alice Rogomenoma Kabre, Madeleine Bisseye, Cyrille Zohoncon, Théodora Mahoukèdè Asshi, Maleki Soubeiga, Serge Théophile Diarra, Birama Traore, Lassina Djigma, Florencia Wendkuuni Ouermi, Djénéba Pietra, Virginio Barro, Nicolas Simpore, Jacques |
author_facet | Ouedraogo, Alice Rogomenoma Kabre, Madeleine Bisseye, Cyrille Zohoncon, Théodora Mahoukèdè Asshi, Maleki Soubeiga, Serge Théophile Diarra, Birama Traore, Lassina Djigma, Florencia Wendkuuni Ouermi, Djénéba Pietra, Virginio Barro, Nicolas Simpore, Jacques |
author_sort | Ouedraogo, Alice Rogomenoma |
collection | PubMed |
description | INTRODUCTION: Herpesvirus EBV, CMV and HHV-6 are viruses that evolve based on pandemic modeling and are responsible for congenital infections causing severe sequelae in infants. This study aims to determine the prevalence of CMV, EBV and HHV-6 among HIV (+) and HIV (-) pregnant women in Ouagadougou. METHODS: In this study 200 blood plasma samples taken from pregnant women, of whom 100 with HIV(+) and 100 with HIV(-), were analyzed using multiplex real-time PCR which detected three infections (EBV, CMV and HHV-6). RESULTS: Out of the 200 samples tested, 18(9.0%) were positive for at least one of the three viruses, 12(6.0%) were positive for EBV, 13(6.5%) were positive for CMV and 12(6.0%) were positive for HHV-6. Among the 18 cases with infections, 10 cases (55.6%) had co-infections of whom 90.0% (9/10) with multiple EBV/CMV/HHV6 infection and 10.0% with EBV/HHV6 co-infection. HHVs infection rate was higher among HIV (-) pregnant women than among HIV (+) pregnant women (12.0% versus 6.0%). Among HIV (+) pregnant women, PCR showed 7.1% (6/85) of HHVs infection in patients who were not treated with ARV against 0% in those treated with ARVs. CONCLUSION: Herpes virus infections are a common condition in pregnant women in Burkina Faso. They may represent a real threat to pregnant women because of complications and risks of infection in infants. |
format | Online Article Text |
id | pubmed-5075482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The African Field Epidemiology Network |
record_format | MEDLINE/PubMed |
spelling | pubmed-50754822016-10-31 Diagnostic moléculaire du Cytomégalovirus (CMV), de l’herpès virus humain de type 6 (HHV6) et d’Epstein-Barr virus (EBV) par PCR en temps réel chez les femmes enceintes VIH séropositives et séronégatives à Ouagadougou, Burkina Faso Ouedraogo, Alice Rogomenoma Kabre, Madeleine Bisseye, Cyrille Zohoncon, Théodora Mahoukèdè Asshi, Maleki Soubeiga, Serge Théophile Diarra, Birama Traore, Lassina Djigma, Florencia Wendkuuni Ouermi, Djénéba Pietra, Virginio Barro, Nicolas Simpore, Jacques Pan Afr Med J Research INTRODUCTION: Herpesvirus EBV, CMV and HHV-6 are viruses that evolve based on pandemic modeling and are responsible for congenital infections causing severe sequelae in infants. This study aims to determine the prevalence of CMV, EBV and HHV-6 among HIV (+) and HIV (-) pregnant women in Ouagadougou. METHODS: In this study 200 blood plasma samples taken from pregnant women, of whom 100 with HIV(+) and 100 with HIV(-), were analyzed using multiplex real-time PCR which detected three infections (EBV, CMV and HHV-6). RESULTS: Out of the 200 samples tested, 18(9.0%) were positive for at least one of the three viruses, 12(6.0%) were positive for EBV, 13(6.5%) were positive for CMV and 12(6.0%) were positive for HHV-6. Among the 18 cases with infections, 10 cases (55.6%) had co-infections of whom 90.0% (9/10) with multiple EBV/CMV/HHV6 infection and 10.0% with EBV/HHV6 co-infection. HHVs infection rate was higher among HIV (-) pregnant women than among HIV (+) pregnant women (12.0% versus 6.0%). Among HIV (+) pregnant women, PCR showed 7.1% (6/85) of HHVs infection in patients who were not treated with ARV against 0% in those treated with ARVs. CONCLUSION: Herpes virus infections are a common condition in pregnant women in Burkina Faso. They may represent a real threat to pregnant women because of complications and risks of infection in infants. The African Field Epidemiology Network 2016-07-12 /pmc/articles/PMC5075482/ /pubmed/27800078 http://dx.doi.org/10.11604/pamj.2016.24.223.9406 Text en © Alice Rogomenoma Ouedraogo et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Ouedraogo, Alice Rogomenoma Kabre, Madeleine Bisseye, Cyrille Zohoncon, Théodora Mahoukèdè Asshi, Maleki Soubeiga, Serge Théophile Diarra, Birama Traore, Lassina Djigma, Florencia Wendkuuni Ouermi, Djénéba Pietra, Virginio Barro, Nicolas Simpore, Jacques Diagnostic moléculaire du Cytomégalovirus (CMV), de l’herpès virus humain de type 6 (HHV6) et d’Epstein-Barr virus (EBV) par PCR en temps réel chez les femmes enceintes VIH séropositives et séronégatives à Ouagadougou, Burkina Faso |
title | Diagnostic moléculaire du Cytomégalovirus (CMV), de l’herpès virus humain de type 6 (HHV6) et d’Epstein-Barr virus (EBV) par PCR en temps réel chez les femmes enceintes VIH séropositives et séronégatives à Ouagadougou, Burkina Faso |
title_full | Diagnostic moléculaire du Cytomégalovirus (CMV), de l’herpès virus humain de type 6 (HHV6) et d’Epstein-Barr virus (EBV) par PCR en temps réel chez les femmes enceintes VIH séropositives et séronégatives à Ouagadougou, Burkina Faso |
title_fullStr | Diagnostic moléculaire du Cytomégalovirus (CMV), de l’herpès virus humain de type 6 (HHV6) et d’Epstein-Barr virus (EBV) par PCR en temps réel chez les femmes enceintes VIH séropositives et séronégatives à Ouagadougou, Burkina Faso |
title_full_unstemmed | Diagnostic moléculaire du Cytomégalovirus (CMV), de l’herpès virus humain de type 6 (HHV6) et d’Epstein-Barr virus (EBV) par PCR en temps réel chez les femmes enceintes VIH séropositives et séronégatives à Ouagadougou, Burkina Faso |
title_short | Diagnostic moléculaire du Cytomégalovirus (CMV), de l’herpès virus humain de type 6 (HHV6) et d’Epstein-Barr virus (EBV) par PCR en temps réel chez les femmes enceintes VIH séropositives et séronégatives à Ouagadougou, Burkina Faso |
title_sort | diagnostic moléculaire du cytomégalovirus (cmv), de l’herpès virus humain de type 6 (hhv6) et d’epstein-barr virus (ebv) par pcr en temps réel chez les femmes enceintes vih séropositives et séronégatives à ouagadougou, burkina faso |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075482/ https://www.ncbi.nlm.nih.gov/pubmed/27800078 http://dx.doi.org/10.11604/pamj.2016.24.223.9406 |
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