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Gas transport during in vitro and in vivo preclinical testing of inert gas therapies

New gas therapies using inert gases such as xenon and argon are being studied, which require in vitro and in vivo preclinical experiments. Examples of the kinetics of gas transport during such experiments are analyzed in this paper. Using analytical and numerical models, we analyze an in vitro exper...

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Autores principales: Katz, Ira, Palgen, Marc, Murdock, Jacqueline, Martin, Andrew R., Farjot, Géraldine, Caillibotte, Georges
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075678/
https://www.ncbi.nlm.nih.gov/pubmed/27826419
http://dx.doi.org/10.4103/2045-9912.179342
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author Katz, Ira
Palgen, Marc
Murdock, Jacqueline
Martin, Andrew R.
Farjot, Géraldine
Caillibotte, Georges
author_facet Katz, Ira
Palgen, Marc
Murdock, Jacqueline
Martin, Andrew R.
Farjot, Géraldine
Caillibotte, Georges
author_sort Katz, Ira
collection PubMed
description New gas therapies using inert gases such as xenon and argon are being studied, which require in vitro and in vivo preclinical experiments. Examples of the kinetics of gas transport during such experiments are analyzed in this paper. Using analytical and numerical models, we analyze an in vitro experiment for gas transport to a 96 cell well plate and an in vivo delivery to a small animal chamber, where the key processes considered are the wash-in of test gas into an apparatus dead volume, the diffusion of test gas through the liquid media in a well of a cell test plate, and the pharmacokinetics in a rat. In the case of small animals in a chamber, the key variable controlling the kinetics is the chamber wash-in time constant that is a function of the chamber volume and the gas flow rate. For cells covered by a liquid media the diffusion of gas through the liquid media is the dominant mechanism, such that liquid depth and the gas diffusion constant are the key parameters. The key message from these analyses is that the transport of gas during preclinical experiments can be important in determining the true dose as experienced at the site of action in an animal or to a cell.
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spelling pubmed-50756782016-11-08 Gas transport during in vitro and in vivo preclinical testing of inert gas therapies Katz, Ira Palgen, Marc Murdock, Jacqueline Martin, Andrew R. Farjot, Géraldine Caillibotte, Georges Med Gas Res Research Article New gas therapies using inert gases such as xenon and argon are being studied, which require in vitro and in vivo preclinical experiments. Examples of the kinetics of gas transport during such experiments are analyzed in this paper. Using analytical and numerical models, we analyze an in vitro experiment for gas transport to a 96 cell well plate and an in vivo delivery to a small animal chamber, where the key processes considered are the wash-in of test gas into an apparatus dead volume, the diffusion of test gas through the liquid media in a well of a cell test plate, and the pharmacokinetics in a rat. In the case of small animals in a chamber, the key variable controlling the kinetics is the chamber wash-in time constant that is a function of the chamber volume and the gas flow rate. For cells covered by a liquid media the diffusion of gas through the liquid media is the dominant mechanism, such that liquid depth and the gas diffusion constant are the key parameters. The key message from these analyses is that the transport of gas during preclinical experiments can be important in determining the true dose as experienced at the site of action in an animal or to a cell. Medknow Publications & Media Pvt Ltd 2016-04-04 /pmc/articles/PMC5075678/ /pubmed/27826419 http://dx.doi.org/10.4103/2045-9912.179342 Text en Copyright: © 2016 Medical Gas Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Katz, Ira
Palgen, Marc
Murdock, Jacqueline
Martin, Andrew R.
Farjot, Géraldine
Caillibotte, Georges
Gas transport during in vitro and in vivo preclinical testing of inert gas therapies
title Gas transport during in vitro and in vivo preclinical testing of inert gas therapies
title_full Gas transport during in vitro and in vivo preclinical testing of inert gas therapies
title_fullStr Gas transport during in vitro and in vivo preclinical testing of inert gas therapies
title_full_unstemmed Gas transport during in vitro and in vivo preclinical testing of inert gas therapies
title_short Gas transport during in vitro and in vivo preclinical testing of inert gas therapies
title_sort gas transport during in vitro and in vivo preclinical testing of inert gas therapies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075678/
https://www.ncbi.nlm.nih.gov/pubmed/27826419
http://dx.doi.org/10.4103/2045-9912.179342
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