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Antibody Production in Response to Staphylococcal MS-1 Phage Cocktail in Patients Undergoing Phage Therapy

In this study, we investigated the humoral immune response (through the release of IgG, IgA, and IgM antiphage antibodies) to a staphylococcal phage cocktail in patients undergoing experimental phage therapy at the Phage Therapy Unit, Medical Center of the Ludwik Hirszfeld Institute of Immunology an...

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Autores principales: Żaczek, Maciej, Łusiak-Szelachowska, Marzanna, Jończyk-Matysiak, Ewa, Weber-Dąbrowska, Beata, Międzybrodzki, Ryszard, Owczarek, Barbara, Kopciuch, Agnieszka, Fortuna, Wojciech, Rogóż, Paweł, Górski, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075762/
https://www.ncbi.nlm.nih.gov/pubmed/27822205
http://dx.doi.org/10.3389/fmicb.2016.01681
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author Żaczek, Maciej
Łusiak-Szelachowska, Marzanna
Jończyk-Matysiak, Ewa
Weber-Dąbrowska, Beata
Międzybrodzki, Ryszard
Owczarek, Barbara
Kopciuch, Agnieszka
Fortuna, Wojciech
Rogóż, Paweł
Górski, Andrzej
author_facet Żaczek, Maciej
Łusiak-Szelachowska, Marzanna
Jończyk-Matysiak, Ewa
Weber-Dąbrowska, Beata
Międzybrodzki, Ryszard
Owczarek, Barbara
Kopciuch, Agnieszka
Fortuna, Wojciech
Rogóż, Paweł
Górski, Andrzej
author_sort Żaczek, Maciej
collection PubMed
description In this study, we investigated the humoral immune response (through the release of IgG, IgA, and IgM antiphage antibodies) to a staphylococcal phage cocktail in patients undergoing experimental phage therapy at the Phage Therapy Unit, Medical Center of the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy in Wrocław, Poland. We also evaluated whether occurring antiphage antibodies had neutralizing properties toward applied phages (K rate). Among 20 examined patients receiving the MS-1 phage cocktail orally and/or locally, the majority did not show a noticeably higher level of antiphage antibodies in their sera during phage administration. Even in those individual cases with an increased immune response, mostly by induction of IgG and IgM, the presence of antiphage antibodies did not translate into unsatisfactory clinical results of phage therapy. On the other hand, a negative outcome of the treatment occurred in some patients who showed relatively weak production of antiphage antibodies before and during treatment. This may imply that possible induction of antiphage antibodies is not an obstacle to the implementation of phage therapy and support our assumption that the outcome of the phage treatment does not primarily depend on the appearance of antiphage antibodies in sera of patients during therapy. These conclusions are in line with our previous findings. The confirmation of this thesis is of great interest as regards the efficacy of phage therapy in humans.
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spelling pubmed-50757622016-11-07 Antibody Production in Response to Staphylococcal MS-1 Phage Cocktail in Patients Undergoing Phage Therapy Żaczek, Maciej Łusiak-Szelachowska, Marzanna Jończyk-Matysiak, Ewa Weber-Dąbrowska, Beata Międzybrodzki, Ryszard Owczarek, Barbara Kopciuch, Agnieszka Fortuna, Wojciech Rogóż, Paweł Górski, Andrzej Front Microbiol Microbiology In this study, we investigated the humoral immune response (through the release of IgG, IgA, and IgM antiphage antibodies) to a staphylococcal phage cocktail in patients undergoing experimental phage therapy at the Phage Therapy Unit, Medical Center of the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy in Wrocław, Poland. We also evaluated whether occurring antiphage antibodies had neutralizing properties toward applied phages (K rate). Among 20 examined patients receiving the MS-1 phage cocktail orally and/or locally, the majority did not show a noticeably higher level of antiphage antibodies in their sera during phage administration. Even in those individual cases with an increased immune response, mostly by induction of IgG and IgM, the presence of antiphage antibodies did not translate into unsatisfactory clinical results of phage therapy. On the other hand, a negative outcome of the treatment occurred in some patients who showed relatively weak production of antiphage antibodies before and during treatment. This may imply that possible induction of antiphage antibodies is not an obstacle to the implementation of phage therapy and support our assumption that the outcome of the phage treatment does not primarily depend on the appearance of antiphage antibodies in sera of patients during therapy. These conclusions are in line with our previous findings. The confirmation of this thesis is of great interest as regards the efficacy of phage therapy in humans. Frontiers Media S.A. 2016-10-24 /pmc/articles/PMC5075762/ /pubmed/27822205 http://dx.doi.org/10.3389/fmicb.2016.01681 Text en Copyright © 2016 Żaczek, Łusiak-Szelachowska, Jończyk-Matysiak, Weber-Dąbrowska, Międzybrodzki, Owczarek, Kopciuch, Fortuna, Rogóż and Górski. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Żaczek, Maciej
Łusiak-Szelachowska, Marzanna
Jończyk-Matysiak, Ewa
Weber-Dąbrowska, Beata
Międzybrodzki, Ryszard
Owczarek, Barbara
Kopciuch, Agnieszka
Fortuna, Wojciech
Rogóż, Paweł
Górski, Andrzej
Antibody Production in Response to Staphylococcal MS-1 Phage Cocktail in Patients Undergoing Phage Therapy
title Antibody Production in Response to Staphylococcal MS-1 Phage Cocktail in Patients Undergoing Phage Therapy
title_full Antibody Production in Response to Staphylococcal MS-1 Phage Cocktail in Patients Undergoing Phage Therapy
title_fullStr Antibody Production in Response to Staphylococcal MS-1 Phage Cocktail in Patients Undergoing Phage Therapy
title_full_unstemmed Antibody Production in Response to Staphylococcal MS-1 Phage Cocktail in Patients Undergoing Phage Therapy
title_short Antibody Production in Response to Staphylococcal MS-1 Phage Cocktail in Patients Undergoing Phage Therapy
title_sort antibody production in response to staphylococcal ms-1 phage cocktail in patients undergoing phage therapy
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075762/
https://www.ncbi.nlm.nih.gov/pubmed/27822205
http://dx.doi.org/10.3389/fmicb.2016.01681
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