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Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model
What strategies do bacteria employ for adaptation to their hosts and are these strategies different for varied hosts? To date, many studies on the interaction of the bacterium and its host have been published. However, global changes in the bacterial cell in the process of invasion and persistence,...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075909/ https://www.ncbi.nlm.nih.gov/pubmed/27775027 http://dx.doi.org/10.1038/srep35959 |
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author | Matyushkina, Daria Pobeguts, Olga Butenko, Ivan Vanyushkina, Anna Anikanov, Nicolay Bukato, Olga Evsyutina, Daria Bogomazova, Alexandra Lagarkova, Maria Semashko, Tatiana Garanina, Irina Babenko, Vladislav Vakhitova, Maria Ladygina, Valentina Fisunov, Gleb Govorun, Vadim |
author_facet | Matyushkina, Daria Pobeguts, Olga Butenko, Ivan Vanyushkina, Anna Anikanov, Nicolay Bukato, Olga Evsyutina, Daria Bogomazova, Alexandra Lagarkova, Maria Semashko, Tatiana Garanina, Irina Babenko, Vladislav Vakhitova, Maria Ladygina, Valentina Fisunov, Gleb Govorun, Vadim |
author_sort | Matyushkina, Daria |
collection | PubMed |
description | What strategies do bacteria employ for adaptation to their hosts and are these strategies different for varied hosts? To date, many studies on the interaction of the bacterium and its host have been published. However, global changes in the bacterial cell in the process of invasion and persistence, remain poorly understood. In this study, we demonstrated phase transition of the avian pathogen Mycoplasma gallisepticum upon invasion of the various types of eukaryotic cells (human, chicken, and mouse) which was stable during several passages after isolation of intracellular clones and recultivation in a culture medium. It was shown that this phase transition is manifested in changes at the proteomic, genomic and metabolomic levels. Eukaryotic cells induced similar proteome reorganization of M. gallisepticum during infection, despite different origins of the host cell lines. Proteomic changes affected a broad range of processes including metabolism, translation and oxidative stress response. We determined that the activation of glycerol utilization, overproduction of hydrogen peroxide and the upregulation of the SpxA regulatory protein occurred during intracellular infection. We propose SpxA as an important regulator for the adaptation of M. gallisepticum to an intracellular environment. |
format | Online Article Text |
id | pubmed-5075909 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50759092016-10-28 Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model Matyushkina, Daria Pobeguts, Olga Butenko, Ivan Vanyushkina, Anna Anikanov, Nicolay Bukato, Olga Evsyutina, Daria Bogomazova, Alexandra Lagarkova, Maria Semashko, Tatiana Garanina, Irina Babenko, Vladislav Vakhitova, Maria Ladygina, Valentina Fisunov, Gleb Govorun, Vadim Sci Rep Article What strategies do bacteria employ for adaptation to their hosts and are these strategies different for varied hosts? To date, many studies on the interaction of the bacterium and its host have been published. However, global changes in the bacterial cell in the process of invasion and persistence, remain poorly understood. In this study, we demonstrated phase transition of the avian pathogen Mycoplasma gallisepticum upon invasion of the various types of eukaryotic cells (human, chicken, and mouse) which was stable during several passages after isolation of intracellular clones and recultivation in a culture medium. It was shown that this phase transition is manifested in changes at the proteomic, genomic and metabolomic levels. Eukaryotic cells induced similar proteome reorganization of M. gallisepticum during infection, despite different origins of the host cell lines. Proteomic changes affected a broad range of processes including metabolism, translation and oxidative stress response. We determined that the activation of glycerol utilization, overproduction of hydrogen peroxide and the upregulation of the SpxA regulatory protein occurred during intracellular infection. We propose SpxA as an important regulator for the adaptation of M. gallisepticum to an intracellular environment. Nature Publishing Group 2016-10-24 /pmc/articles/PMC5075909/ /pubmed/27775027 http://dx.doi.org/10.1038/srep35959 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Matyushkina, Daria Pobeguts, Olga Butenko, Ivan Vanyushkina, Anna Anikanov, Nicolay Bukato, Olga Evsyutina, Daria Bogomazova, Alexandra Lagarkova, Maria Semashko, Tatiana Garanina, Irina Babenko, Vladislav Vakhitova, Maria Ladygina, Valentina Fisunov, Gleb Govorun, Vadim Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model |
title | Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model |
title_full | Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model |
title_fullStr | Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model |
title_full_unstemmed | Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model |
title_short | Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model |
title_sort | phase transition of the bacterium upon invasion of a host cell as a mechanism of adaptation: a mycoplasma gallisepticum model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075909/ https://www.ncbi.nlm.nih.gov/pubmed/27775027 http://dx.doi.org/10.1038/srep35959 |
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