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Consolidated Bioprocessing for Butyric Acid Production from Rice Straw with Undefined Mixed Culture
Lignocellulosic biomass is a renewable source with great potential for biofuels and bioproducts. However, the cost of cellulolytic enzymes limits the utilization of the low-cost bioresource. This study aimed to develop a consolidated bioprocessing without the need of supplementary cellulase for buty...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5076434/ https://www.ncbi.nlm.nih.gov/pubmed/27822203 http://dx.doi.org/10.3389/fmicb.2016.01648 |
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author | Ai, Binling Chi, Xue Meng, Jia Sheng, Zhanwu Zheng, Lili Zheng, Xiaoyan Li, Jianzheng |
author_facet | Ai, Binling Chi, Xue Meng, Jia Sheng, Zhanwu Zheng, Lili Zheng, Xiaoyan Li, Jianzheng |
author_sort | Ai, Binling |
collection | PubMed |
description | Lignocellulosic biomass is a renewable source with great potential for biofuels and bioproducts. However, the cost of cellulolytic enzymes limits the utilization of the low-cost bioresource. This study aimed to develop a consolidated bioprocessing without the need of supplementary cellulase for butyric acid production from lignocellulosic biomass. A stirred-tank reactor with a working volume of 21 L was constructed and operated in batch and semi-continuous fermentation modes with a cellulolytic butyrate-producing microbial community. The semi-continuous fermentation with intermittent discharging of the culture broth and replenishment with fresh medium achieved the highest butyric acid productivity of 2.69 g/(L· d). In semi-continuous operation mode, the butyric acid and total carboxylic acid concentrations of 16.2 and 28.9 g/L, respectively, were achieved. Over the 21-day fermentation period, their cumulative yields reached 1189 and 2048 g, respectively, corresponding to 41 and 74% of the maximum theoretical yields based on the amount of NaOH pretreated rice straw fed in. This study demonstrated that an undefined mixed culture-based consolidated bioprocessing for butyric acid production can completely eliminate the cost of supplementary cellulolytic enzymes. |
format | Online Article Text |
id | pubmed-5076434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50764342016-11-07 Consolidated Bioprocessing for Butyric Acid Production from Rice Straw with Undefined Mixed Culture Ai, Binling Chi, Xue Meng, Jia Sheng, Zhanwu Zheng, Lili Zheng, Xiaoyan Li, Jianzheng Front Microbiol Microbiology Lignocellulosic biomass is a renewable source with great potential for biofuels and bioproducts. However, the cost of cellulolytic enzymes limits the utilization of the low-cost bioresource. This study aimed to develop a consolidated bioprocessing without the need of supplementary cellulase for butyric acid production from lignocellulosic biomass. A stirred-tank reactor with a working volume of 21 L was constructed and operated in batch and semi-continuous fermentation modes with a cellulolytic butyrate-producing microbial community. The semi-continuous fermentation with intermittent discharging of the culture broth and replenishment with fresh medium achieved the highest butyric acid productivity of 2.69 g/(L· d). In semi-continuous operation mode, the butyric acid and total carboxylic acid concentrations of 16.2 and 28.9 g/L, respectively, were achieved. Over the 21-day fermentation period, their cumulative yields reached 1189 and 2048 g, respectively, corresponding to 41 and 74% of the maximum theoretical yields based on the amount of NaOH pretreated rice straw fed in. This study demonstrated that an undefined mixed culture-based consolidated bioprocessing for butyric acid production can completely eliminate the cost of supplementary cellulolytic enzymes. Frontiers Media S.A. 2016-10-24 /pmc/articles/PMC5076434/ /pubmed/27822203 http://dx.doi.org/10.3389/fmicb.2016.01648 Text en Copyright © 2016 Ai, Chi, Meng, Sheng, Zheng, Zheng and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Ai, Binling Chi, Xue Meng, Jia Sheng, Zhanwu Zheng, Lili Zheng, Xiaoyan Li, Jianzheng Consolidated Bioprocessing for Butyric Acid Production from Rice Straw with Undefined Mixed Culture |
title | Consolidated Bioprocessing for Butyric Acid Production from Rice Straw with Undefined Mixed Culture |
title_full | Consolidated Bioprocessing for Butyric Acid Production from Rice Straw with Undefined Mixed Culture |
title_fullStr | Consolidated Bioprocessing for Butyric Acid Production from Rice Straw with Undefined Mixed Culture |
title_full_unstemmed | Consolidated Bioprocessing for Butyric Acid Production from Rice Straw with Undefined Mixed Culture |
title_short | Consolidated Bioprocessing for Butyric Acid Production from Rice Straw with Undefined Mixed Culture |
title_sort | consolidated bioprocessing for butyric acid production from rice straw with undefined mixed culture |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5076434/ https://www.ncbi.nlm.nih.gov/pubmed/27822203 http://dx.doi.org/10.3389/fmicb.2016.01648 |
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